CAZyme Information: PGTG_09481-t26_1-p1

Basic Information

• Species: Puccinia graminis
• Lineage: Basidiomycota; Pucciniomycetes; ; Pucciniaceae; Puccinia; Puccinia graminis
• CAZyme ID: PGTG_09481-t26_1-p1
• CAZy Family: GH26
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
598		65264.19	9.1800

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_PgraminisCRL75-36-700-3	16309	418459	509	15800

• Gene Location: location=DS178287:746611-748843(-)

Full Sequence      

MFVITIQYSFYFIHLVIAVICLFLDPVRTGANAQSHNPPEVVGPFGARSRTDAGPPRWSR
IVKGHTGVAAMQITVVSDKYALILDKVEHNPLKINGHRAWAALYNFETDEVTPLDLKSNS
FCAGGSFLGNGTLINFGGNPVGKAPLRTGNFGPTDGLQSIRFYTPCDDGKCSVAEFDSIK
LTSPRWYATATRLADGSIMIAGGSKRGAFRNNAAINNPTIEYFPPRQLNFPTNSGKKQIY
SPFLERTLVANLFPIVITLPEPGLVFLAANRDAIIYNYTSNYEFRLPRIPNGVRVTYPMT
GGGILLPLSPQNGYKPEVLICGGSDLDDTLETRLIKASAPASTQCVRMVLTKSGIKKGWI
VEHMPEGRIMPDMIMMPDGKVLIVNGAKSGVGGYGSLEKMTGNSNADNPSFTPVLYDPDA
PLGQRFSSEGLPTTNIARLYHSVATLTPSGLVMLAGSNPNPDVSTANYRTEYRVEWLSPP
YIKHPNRPKISSLPKLANYKEKIIVKLVGMDLKLAEQKVEAVLLDFGFVTHSTHMNSRLV
KLITSVDSDDNELQVVMPPLPGIYPPGYGWLFVVINGIPSPGKRIMIGSGKLDFEDAR

Enzyme Prediction     

No EC number prediction in PGTG_09481-t26_1-p1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
AA5	62	593	3.7e-187	0.9502664298401421

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
399910	Glyoxal_oxid_N	4.94e-45	99	323	46	243	Glyoxal oxidase N-terminus. This family represents the N-terminus (approximately 300 residues) of a number of plant and fungal glyoxal oxidase enzymes. Glyoxal oxidase catalyzes the oxidation of aldehydes to carboxylic acids, coupled with reduction of dioxygen to hydrogen peroxide. It is an essential component of the extracellular lignin degradation pathways of the wood-rot fungus Phanerochaete chrysosporium.
199882	E_set_GO_C	9.42e-29	485	587	4	103	C-terminal Early set domain associated with the catalytic domain of galactose oxidase. E or "early" set domains are associated with the catalytic domain of galactose oxidase at the C-terminal end. Galactose oxidase is an extracellular monomeric enzyme which catalyzes the stereospecific oxidation of a broad range of primary alcohol substrates and possesses a unique mononuclear copper site essential for catalyzing a two-electron transfer reaction during the oxidation of primary alcohols to corresponding aldehydes. The second redox active center necessary for the reaction was found to be situated at a tyrosine residue. The C-terminal domain of galactose oxidase may be related to the immunoglobulin and/or fibronectin type III superfamilies. These domains are associated with different types of catalytic domains at either the N-terminal or C-terminal end and may be involved in homodimeric/tetrameric/dodecameric interactions. Members of this family include members of the alpha amylase family, sialidase, galactose oxidase, cellulase, cellulose, hyaluronate lyase, chitobiase, and chitinase, among others.
401164	DUF1929	2.46e-26	494	587	3	91	Domain of unknown function (DUF1929). Members of this family adopt a secondary structure consisting of a bundle of seven, mostly antiparallel, beta-strands surrounding a hydrophobic core. The 7 strands are arranged in 2 sheets, in a Greek-key topology. Their precise function, has not, as yet, been defined, though they are mostly found in sugar-utilising enzymes, such as galactose oxidase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QRW11955.1|AA5_1	1.32e-205	58	590	140	663
QRV83232.1|AA5_1	1.32e-205	58	590	140	663
QRV97414.1|AA5_1	2.65e-205	58	590	140	663
ABD61577.1|AA5_1	5.81e-204	58	591	221	749
QRW24733.1|AA5_1	6.49e-199	27	590	173	721

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1T2X_A	5.91e-17	29	587	130	637	Glactose oxidase C383S mutant identified by directed evolution [Fusarium sp.]
1GOF_A	1.03e-16	29	587	130	637	NOVEL THIOETHER BOND REVEALED BY A 1.7 ANGSTROMS CRYSTAL STRUCTURE OF GALACTOSE OXIDASE [Hypomyces rosellus],1GOG_A NOVEL THIOETHER BOND REVEALED BY A 1.7 ANGSTROMS CRYSTAL STRUCTURE OF GALACTOSE OXIDASE [Hypomyces rosellus],1GOH_A NOVEL THIOETHER BOND REVEALED BY A 1.7 ANGSTROMS CRYSTAL STRUCTURE OF GALACTOSE OXIDASE [Hypomyces rosellus],2EIE_A Chain A, Galactose oxidase [Fusarium graminearum],2JKX_A Chain A, GALACTOSE OXIDASE [Fusarium graminearum],2VZ1_A Chain A, GALACTOSE OXIDASE [Fusarium graminearum],2VZ3_A Chain A, Galactose Oxidase [Fusarium graminearum]
2EIB_A	1.03e-16	29	587	130	637	Chain A, Galactose oxidase [Fusarium graminearum]
2EIC_A	1.03e-16	29	587	130	637	Chain A, Galactose oxidase [Fusarium graminearum]
2EID_A	1.03e-16	29	587	130	637	Chain A, Galactose oxidase [Fusarium graminearum]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q01772|GLOX_PHACH	4.75e-131	56	590	24	552	Aldehyde oxidase GLOX OS=Phanerodontia chrysosporium OX=2822231 GN=GLX PE=1 SV=1
sp|Q3HRQ2|GLOX_VITPS	9.50e-70	55	587	14	521	Aldehyde oxidase GLOX OS=Vitis pseudoreticulata OX=231512 GN=GLOX PE=2 SV=1
sp|Q9FYG4|GLOX1_ARATH	1.46e-64	93	587	148	614	Aldehyde oxidase GLOX1 OS=Arabidopsis thaliana OX=3702 GN=GLOX1 PE=2 SV=1
sp|A0A2H4HHY6|GLOX_ARTAN	3.41e-46	65	587	76	593	Putative aldehyde oxidase Art an 7 OS=Artemisia annua OX=35608 PE=1 SV=1
sp|D4AUF1|WSCD1_ARTBC	6.29e-40	114	588	477	904	WSC domain-containing protein ARB_07867 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_07867 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.457244	0.542744	CS pos: 29-30. Pr: 0.5166

TMHMM  Annotations     

Start	End
2	24