CAZyme Information: PADG_05129-t30_1-p1

Basic Information

• Species: Paracoccidioides brasiliensis
• Lineage: Ascomycota; Eurotiomycetes; ; NA; Paracoccidioides; Paracoccidioides brasiliensis
• CAZyme ID: PADG_05129-t30_1-p1
• CAZy Family: GH63
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1494	KN275962|CGC3	167661.47	5.6841

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_PbrasiliensisPb18	8522	502780	132	8390

• Gene Location: location=KN275962:103007-107758(-)

Full Sequence      

MVFKYIFSPWRLGGLALLATTFLFTPIASAGPLVNVALTASFNAAPYLVELLETAADENS
TSYFPLLDHIAEGAFTEITTERDLYERFTQILQDNGHLTDAESISSFKFALSLRSTAPRI
EAHYQYYHTSVEPSLVVAQDAVCTVWVYYDGYQYCSPSLKYAQQSVSGDQHERVLPFDRV
FGDPSLPALVLYADVTSPMFSGFHQELSRKAREGQFSYKIRYRSSGTEASKPIFVSGYGV
ELALKRTDYVVIDDRDAEQSVLKDTDAAQTTLAPVDDAKNEPPTDLKPLSASEVSTLGIN
AASFVMSSDDPLATLLRLSQDFPRHSSAIAGRNATPEFIKELHENQAAQLPVGLNTMWIN
GMQIDPNNIDAFFLLSHLRQERKLINRFREFGLSARQAVGLFSDPAVSKTQAADDSLRYQ
YRDDFEGGGVIIWMNNLEKDHQYDGWTKSLNSLLRPIYPGQLPPIRRDIHNVVIPVDLTS
PKDIEIVVRNMQTLVKRKIPIRFGFVPLLQNPNSLEQARVAHYLLDTYGIEALVTYLQAS
YTANKTASPDQASFTAALKDHKVRDGHNPVTFKDVLTSENYDPILASTKTYLQRLAIEGK
EPTFFVNGVAFVRDENFMQYIITAVSNDMDKIQKAVFEGDLEEDVWVPSYFLQGALQTRN
PLLIPEDPRSIRIVDLNKIYENNKDIFDTLLRIPATSKSEHSMLDWTSIILIADLESESG
VKQLRHLLELHEKHPGVETLLLHNGEASSVSKGLLTRLHSVRHGRDLDPAAVAATLDALV
SGEDTPGSDANLATAYWNSLQELVREIGIGAGEYGMVINSRIIGPLPSSTVFDALDLEYM
FAYERSKRTEVVAQAALDLGLEDKISDPLSLAKLLSLMSLSTASDIPEGILNSVSNVRSN
LYKKWNGTHSAISISNSDDPSIYIVAAVDPATETAQRCIPILKVLSELNGVNLKIFLTPR
EAIKELPIKRFYQYILKSTPSFSEDGSIAKPQATFHGIPGDALLNLGMDVPHSWLVAPKE
SIYDLDNIKLSSLKEGANVDALYELEHILIEGHSWDVTTRTAPRGVQLLLGTEKKPHFAD
TIIMANLGYFQFKAQPGCWKITLKPGQSERIFRLDSVGGKGHRPTPGDENNDVALLSFRG
KTLYPRLSRRPGHEKDDVLDPDPKPNSAKDYLSRGLSFASSVLSSVTGPKEKHADINIFS
VASGHLYERMLNIMMVSVMKHTGHSVKFWFIEQFLSPSFKSFLPHLAAEYGFSYEMVTYK
WPNWLHEQQEKQRIIWGYKILFLDVLFPLSLDKVIFVDADQIVRTDMYELVTLDLEGAPY
GFTPMCDSRTSMEGFRFWKQGYWKKFLKGLPYHISALYVVDLNQFRAIAAGDRLRGQYHT
LAIDPNSLSNLDQDLPNNMQRVLPIKSLPQDWLWCETWCSDEALKTAKTIDLCNNPLTKE
PKLDRARRQVPEWTVYDEEIAAVQRRVMEGKKGKEDGEEGRGSDGKKERRKDEL

Enzyme Prediction     

EC	2.4.1.-:29

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT24	1196	1443	1.3e-131	0.9959677419354839

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
408203	Glyco_transf_24	0.0	1196	1463	1	268	Glucosyltransferase 24. This is the catalytic domain found in UDP-glucose:glycoprotein glucosyltransferase (UGGT). This domain belongs to glucosyltransferase 24 family (GT24) A-type domain. The GT domain displays the expected glycosyltransferase type A (GT-A) fold.
133054	GT8_HUGT1_C_like	1.69e-178	1196	1443	1	248	The C-terminal domain of HUGT1-like is highly homologous to the GT 8 family. C-terminal domain of glycoprotein glucosyltransferase (UGT). UGT is a large glycoprotein whose C-terminus contains the catalytic activity. This catalytic C-terminal domain is highly homologous to Glycosyltransferase Family 8 (GT 8) and contains the DXD motif that coordinates donor sugar binding, characteristic for Family 8 glycosyltransferases. GT 8 proteins are retaining enzymes based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. The non-catalytic N-terminal portion of the human UTG1 (HUGT1) has been shown to monitor the protein folding status and activate its glucosyltransferase activity.
408201	Thioredoxin_14	7.82e-96	419	663	1	248	Thioredoxin-like domain. This is the third out of four TRXL(thioredoxin-like) domains found in UDP-glucose:glycoprotein glucosyltransferase (UGGT).
399437	UDP-g_GGTase	9.00e-59	1051	1160	1	109	UDP-glucose:Glycoprotein Glucosyltransferase. UDP-g_GGTase is an important, central component of the QC system in the ER for checking that glycoproteins are folded correctly. This QC prevents incorrectly folded glycoproteins from leaving the ER.
132996	Glyco_transf_8	2.24e-57	1196	1443	1	246	Members of glycosyltransferase family 8 (GT-8) are involved in lipopolysaccharide biosynthesis and glycogen synthesis. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. GT-8 comprises enzymes with a number of known activities: lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, glycogenin glucosyltransferase, and N-acetylglucosaminyltransferase. GT-8 enzymes contains a conserved DXD motif which is essential in the coordination of a catalytic divalent cation, most commonly Mn2+.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QSS69256.1|GT24	0.0	1	1469	1	1468
QSS51253.1|GT24	0.0	1	1472	1	1471
QSS63494.1|GT24	0.0	5	1469	16	1479
QRD91624.1|GT24	0.0	8	1467	9	1459
QMW47293.1|GT24	0.0	8	1467	9	1459

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5MU1_A	0.0	29	1471	6	1455	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5MZO_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TRF_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
5NV4_A	0.0	29	1471	6	1455	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
5Y7O_A	0.0	34	1165	6	1129	Crystal structure of folding sensor region of UGGT from Thermomyces dupontii [Thermomyces dupontii],5Y7O_B Crystal structure of folding sensor region of UGGT from Thermomyces dupontii [Thermomyces dupontii]
6TRT_A	0.0	29	1471	6	1455	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TS2_A	0.0	29	1471	6	1221	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_C Chain C, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_D Chain D, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q09140|UGGG_SCHPO	3.94e-290	23	1463	12	1424	UDP-glucose:glycoprotein glucosyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=gpt1 PE=1 SV=2
sp|Q9NYU2|UGGG1_HUMAN	9.05e-243	10	1471	23	1531	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Homo sapiens OX=9606 GN=UGGT1 PE=1 SV=3
sp|Q6P5E4|UGGG1_MOUSE	1.22e-241	18	1464	31	1524	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Mus musculus OX=10090 GN=Uggt1 PE=1 SV=4
sp|Q9JLA3|UGGG1_RAT	2.39e-241	10	1464	23	1524	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Rattus norvegicus OX=10116 GN=Uggt1 PE=1 SV=2
sp|Q09332|UGGG_DROME	2.82e-237	38	1467	34	1511	UDP-glucose:glycoprotein glucosyltransferase OS=Drosophila melanogaster OX=7227 GN=Uggt PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.027958	0.972021	CS pos: 30-31. Pr: 0.9337

TMHMM  Annotations     

Start	End
12	34