CAZyme Information: P170DRAFT_437975-t37_1-p1

Basic Information

• Species: Aspergillus steynii
• Lineage: Ascomycota; Eurotiomycetes; ; Aspergillaceae; Aspergillus; Aspergillus steynii
• CAZyme ID: P170DRAFT_437975-t37_1-p1
• CAZy Family: GH31
• CAZyme Description: putative mannosyl-oligosaccharide alpha-1,2-mannosidase 1B

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
508		56457.11	5.5528

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AsteyniiIBT23096	13430	1392250	235	13195

• Gene Location: location=MSFO01000005:2529474-2531104(+)

Full Sequence      

MHLPSLLPLTALAFVTPSLAYPQHNHEQPLLKPEKSQSRADTIKEAFVHAWEGYYKHAFP
NDELHPISNGFGNSRNGWGASAVDALSTAIIMGNATIVDQILDHVAKVDYSKTTSMVSLF
ETTIRYLGGMISGYDLLKGPASDLIKDKSKVETLLTQSKNLADILKFAFDTPSGVPYNNV
NITSHDNDHATTNGLAVTGTLVLEWTRLSDLTGNTEYAKLSQKAQSYLLDPQPQEAEPFP
GLVGSHIDIRTGKFSDAQVSWNGGADSFYEYLIKMYVYDPKRFGKYKERWVAAAESSIKH
LASHPEPRPDLTFLASYNNGNLGLSSQHLTCFDGGSFLLGGTVLDRDDFIKFGLDLVNGC
HTTYNETLTGIGPETFSWNPNNVPDSQKALYERAGFYIGSSVYILRPEVIESFYYAWRVT
GEEKYREWVWNAFVAVNKYCRTDSGFAGLKNVNAENGGGKDDNQESFIFAEVLKYVYLAH
SDDEAWQVQKGQGNKFVYNTEAHPVQVA

Enzyme Prediction     

EC	3.2.1.113:12

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH47	46	506	2.1e-138	0.9955156950672646

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
396217	Glyco_hydro_47	0.0	46	504	1	450	Glycosyl hydrolase family 47. Members of this family are alpha-mannosidases that catalyze the hydrolysis of the terminal 1,2-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man(9)(GlcNAc)(2).
240427	PTZ00470	2.08e-125	34	508	67	519	glycoside hydrolase family 47 protein; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BAE57653.1|GH47|3.2.1.113	8.34e-306	1	508	1	509
BAC07247.1|GH47|3.2.1.113	8.34e-306	1	508	1	509
QRD82359.1|GH47	8.34e-306	1	508	1	509
QMW39988.1|GH47	8.34e-306	1	508	1	509
QMW27917.1|GH47	8.34e-306	1	508	1	509

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1KKT_A	2.89e-270	1	508	1	509	Structure of P. citrinum alpha 1,2-mannosidase reveals the basis for differences in specificity of the ER and Golgi Class I enzymes [Penicillium citrinum],1KKT_B Structure of P. citrinum alpha 1,2-mannosidase reveals the basis for differences in specificity of the ER and Golgi Class I enzymes [Penicillium citrinum],1KRE_A Structure Of P. Citrinum Alpha 1,2-Mannosidase Reveals The Basis For Differences In Specificity Of The Er And Golgi Class I Enzymes [Penicillium citrinum],1KRE_B Structure Of P. Citrinum Alpha 1,2-Mannosidase Reveals The Basis For Differences In Specificity Of The Er And Golgi Class I Enzymes [Penicillium citrinum],1KRF_A Structure Of P. Citrinum Alpha 1,2-mannosidase Reveals The Basis For Differences In Specificity Of The Er And Golgi Class I Enzymes [Penicillium citrinum],1KRF_B Structure Of P. Citrinum Alpha 1,2-mannosidase Reveals The Basis For Differences In Specificity Of The Er And Golgi Class I Enzymes [Penicillium citrinum]
2RI8_A	3.26e-265	36	508	2	474	Penicillium citrinum alpha-1,2-mannosidase complex with glycerol [Penicillium citrinum],2RI8_B Penicillium citrinum alpha-1,2-mannosidase complex with glycerol [Penicillium citrinum],2RI9_A Penicillium citrinum alpha-1,2-mannosidase in complex with a substrate analog [Penicillium citrinum],2RI9_B Penicillium citrinum alpha-1,2-mannosidase in complex with a substrate analog [Penicillium citrinum]
1HCU_A	1.16e-160	38	507	14	492	Chain A, ALPHA-1,2-MANNOSIDASE [Trichoderma reesei],1HCU_B Chain B, ALPHA-1,2-MANNOSIDASE [Trichoderma reesei],1HCU_C Chain C, ALPHA-1,2-MANNOSIDASE [Trichoderma reesei],1HCU_D Chain D, ALPHA-1,2-MANNOSIDASE [Trichoderma reesei]
5KKB_A	3.50e-68	43	507	26	467	Structure of mouse Golgi alpha-1,2-mannosidase IA and Man9GlcNAc2-PA complex [Mus musculus],5KKB_B Structure of mouse Golgi alpha-1,2-mannosidase IA and Man9GlcNAc2-PA complex [Mus musculus]
1NXC_A	4.37e-68	43	507	24	465	Structure of mouse Golgi alpha-1,2-mannosidase IA reveals the molecular basis for substrate specificity among Class I enzymes (family 47 glycosidases) [Mus musculus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q2ULB2|MNS1B_ASPOR	1.48e-306	1	508	1	509	Mannosyl-oligosaccharide alpha-1,2-mannosidase 1B OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=mns1B PE=1 SV=1
sp|B8N417|MNS1B_ASPFN	1.48e-306	1	508	1	509	Probable mannosyl-oligosaccharide alpha-1,2-mannosidase 1B OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=mns1B PE=3 SV=2
sp|Q0D076|MNS1B_ASPTN	9.23e-305	1	507	1	507	Probable mannosyl-oligosaccharide alpha-1,2-mannosidase 1B OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=mns1B PE=3 SV=1
sp|A2QAS2|MNS1B_ASPNC	8.34e-293	1	508	1	511	Probable mannosyl-oligosaccharide alpha-1,2-mannosidase 1B OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=mns1B PE=3 SV=1
sp|Q12563|MNS1B_ASPPH	3.39e-292	1	508	1	511	Mannosyl-oligosaccharide alpha-1,2-mannosidase 1B OS=Aspergillus phoenicis OX=5063 GN=mns1B PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000229	0.999762	CS pos: 20-21. Pr: 0.9845

TMHMM  Annotations     

There is no transmembrane helices in P170DRAFT_437975-t37_1-p1.