CAZyme Information: P170DRAFT_389858-t37_1-p1

Basic Information

• Species: Aspergillus steynii
• Lineage: Ascomycota; Eurotiomycetes; ; Aspergillaceae; Aspergillus; Aspergillus steynii
• CAZyme ID: P170DRAFT_389858-t37_1-p1
• CAZy Family: GH114
• CAZyme Description: putative UDP-glucose:glyco protein glucosyltransferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1495		167244.16	4.7417

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AsteyniiIBT23096	13430	1392250	235	13195

• Gene Location: location=MSFO01000007:1257129-1261848(+)

Full Sequence      

MIFNSNSVASWRLTAIVAVVLACLVRASPSVNVALQASFDAAPYLIELLETAAEENSTSY
FPLLDRVADGAFDDAVSDKQIYDRFLQIVDEDGHLKTPESLSSFKLSLAIRSASPRITAH
YQYYNASVQHSLMVAQDAVCPVWVHSEGKQYCSSTLERAQQDVLGEMDSRELPFDRVLGD
LSLPPAILYADVATPMFKDFHHTLSALAKEGQISYRVRYRPPQHWSPRPLFVSGYGVELA
LKRTDYIVIDDRAADQQGSNNAEPSDSEGDAPDDLRPLSSSEVARLGVNAVSYVMNSDDP
LDTLVKLSQDFPKYSARVASRNASTELLEDIRSSRLQMLPAGMNVMWINGVQIDSRQIDA
FSLLDHLRRERRLIEKFRDIGVSAQEAVSLLSHEFLGEALAQDAPQRYNYLDDIEGGRVL
IWLNDLEKDSRYQSWPQELEAFLQRTYPGQLPAVRRNANNIVVPVDLSSLEDLNFVVTTM
SAFVKKGIPARFAIVPIASSVGQKSQVRVAHYLQETYGLSSLLQYLEESLDKNKAASADK
GCFETATKERKVRSDRKELSFEEVLKSDDLENVVSQAVHYQYRLGITADASQSFINGAPV
ARKSNWMQEVSIRISRDLQQIQQGIADGEIEEATSLPGVFLSKAFPRRSQWVIPEDPKDI
RVVDLTKVSDSQKSALSETPLIASTNGDALDSAYLIVVGDFDSESGFQLLTAALEHREKH
NEVEVLFIHNAEKQTSAKGGSTTLYRSIKTGKQIEPAQILANIGSAAISPPSAEEADEIA
QFWAGQQSLARELGFASGVNGVVINGRAIGPIQHDVTSDDLGQLLVYEQEKRIGPVARAC
KDLGFGSNIPGPLAFAKLTSLTTLSTVSDVPEGIFESTSDIRMNLFNKWNDSRSVLTVSN
SEDPAITILASIDPSSETAQRWLPILKVLSKLASVRVRLFLNPREQIQELPTKRFYRYVL
DSEPSFNDNGSISRPTASFRGVPVEALLTLGMDVPSSWLVAPQDSIHDLDNIKLSSLNSD
SDVETVYALEHILIEGHSRDLTTKSPPRGVQLNIGTDNNPDYADTIIMANLGYFQFKTQP
GLWKINLKPGSSERIFNLDSVGGLGYSPQPGDDNNEVALLSFQGKTLFPRLSRKKGHEME
DVLETGPKAGTAMDYVSKGFSFASGVLSSVGVGSKGSLSQKQADINIFSVASGHLYERML
NIMMVSVMKNTKHTVKFWFIEQFLSPSFKSFLPHLAAEYGFQYEMVTFKWPHWLRAQREK
QREIWGYKILFLDVLFPLNLDKVIFVDADQVVRTDMHDLVTLDLEGAPYAFTPMCDSREE
IEGFRFWKQGYWKSFLRGQPYHISALYVVDLNQFRALAAGDRLRGQYQMLSADPNSLSNL
DQDLPNHMQHHIPIKSLPQEWLWCETWCSDESLSQARTIDLCNNPQTKEPKLDRARRQVP
EWTEYDDEIAALSNRVDVERQREEELRAESRQEEEQEEDDSDDVDEDPAWIKDEL

Enzyme Prediction     

EC	2.4.1.-:29

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT24	1185	1432	4.3e-134	0.9959677419354839

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
133054	GT8_HUGT1_C_like	0.0	1185	1432	1	248	The C-terminal domain of HUGT1-like is highly homologous to the GT 8 family. C-terminal domain of glycoprotein glucosyltransferase (UGT). UGT is a large glycoprotein whose C-terminus contains the catalytic activity. This catalytic C-terminal domain is highly homologous to Glycosyltransferase Family 8 (GT 8) and contains the DXD motif that coordinates donor sugar binding, characteristic for Family 8 glycosyltransferases. GT 8 proteins are retaining enzymes based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. The non-catalytic N-terminal portion of the human UTG1 (HUGT1) has been shown to monitor the protein folding status and activate its glucosyltransferase activity.
408203	Glyco_transf_24	0.0	1185	1452	1	268	Glucosyltransferase 24. This is the catalytic domain found in UDP-glucose:glycoprotein glucosyltransferase (UGGT). This domain belongs to glucosyltransferase 24 family (GT24) A-type domain. The GT domain displays the expected glycosyltransferase type A (GT-A) fold.
408201	Thioredoxin_14	7.90e-82	408	652	1	248	Thioredoxin-like domain. This is the third out of four TRXL(thioredoxin-like) domains found in UDP-glucose:glycoprotein glucosyltransferase (UGGT).
408199	Thioredoxin_12	5.80e-63	41	226	1	183	Thioredoxin-like domain. This is one of four TRXL(thioredoxin-like) domains found in UDP-glucose:glycoprotein glucosyltransferase (UGGT).
399437	UDP-g_GGTase	1.06e-58	1035	1144	1	109	UDP-glucose:Glycoprotein Glucosyltransferase. UDP-g_GGTase is an important, central component of the QC system in the ER for checking that glycoproteins are folded correctly. This QC prevents incorrectly folded glycoproteins from leaving the ER.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QRD91624.1|GT24	0.0	1	1495	1	1487
QMW47293.1|GT24	0.0	1	1495	1	1487
QMW35231.1|GT24	0.0	1	1495	1	1487
BAE65008.1|GT24|2.4.1.-	0.0	1	1495	1	1487
UDD64319.1|GT24	0.0	1	1495	1	1487

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5MU1_A	0.0	25	1462	5	1457	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5MZO_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TRF_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
5NV4_A	0.0	25	1462	5	1457	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
5Y7O_A	0.0	30	1150	5	1130	Crystal structure of folding sensor region of UGGT from Thermomyces dupontii [Thermomyces dupontii],5Y7O_B Crystal structure of folding sensor region of UGGT from Thermomyces dupontii [Thermomyces dupontii]
6TRT_A	0.0	25	1462	5	1457	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TS2_A	0.0	25	1462	5	1223	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_C Chain C, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_D Chain D, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q09140|UGGG_SCHPO	1.30e-276	27	1452	18	1424	UDP-glucose:glycoprotein glucosyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=gpt1 PE=1 SV=2
sp|Q09332|UGGG_DROME	2.60e-233	35	1457	34	1512	UDP-glucose:glycoprotein glucosyltransferase OS=Drosophila melanogaster OX=7227 GN=Uggt PE=1 SV=2
sp|Q6P5E4|UGGG1_MOUSE	2.11e-232	28	1452	44	1523	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Mus musculus OX=10090 GN=Uggt1 PE=1 SV=4
sp|Q9NYU2|UGGG1_HUMAN	5.08e-230	28	1461	44	1532	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Homo sapiens OX=9606 GN=UGGT1 PE=1 SV=3
sp|Q9JLA3|UGGG1_RAT	1.86e-228	28	1452	44	1523	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Rattus norvegicus OX=10116 GN=Uggt1 PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000233	0.999748	CS pos: 27-28. Pr: 0.9780

TMHMM  Annotations     

There is no transmembrane helices in P170DRAFT_389858-t37_1-p1.