CAZyme Information: P170DRAFT_382675-t37_1-p1

Basic Information

• Species: Aspergillus steynii
• Lineage: Ascomycota; Eurotiomycetes; ; Aspergillaceae; Aspergillus; Aspergillus steynii
• CAZyme ID: P170DRAFT_382675-t37_1-p1
• CAZy Family: GH10
• CAZyme Description: rhamnogalacturonate lyase B

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
665		74209.26	6.5413

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AsteyniiIBT23096	13430	1392250	235	13195

• Gene Location: location=MSFO01000003:4211540-4213534(+)

Full Sequence      

MRALQFPTLAVVATLASQTHANLQTAQNSTHISVNNDHLSVTLAKSSGHIIDLTLDGQDL
LGPASGNSGKGPYLDCSCVPSGFWTPGGSANAQVQLINGTDSSGTAYAGLIMSDTYAATN
QSLSQYFFLRDGETGLHAFSRLTYFNASLPFLRGLGEMRTLFRPNTNLWTHFSTSDTNHG
PMPSSSAYSSATTVQDATSYLGNTPSDPYVSQYSDYFTKYTLAESWRAHDVHGHFADGSH
SADGSVFGAWLVHNTRESYYGGPLHSDLVVDGIVYNYMVSGHHGAPVPNITHGFDRTWGP
QFYYFNKGEKGTGLEELRKDAARFADPEWNADFYDDIARHVPNLVASKGRTTFEGKVKVP
KGAKRPIVVLSENEQDFQLNVFETESRQYWAEIDESGRFSIPRVVKGTYRVTIYADGVFG
WFIKDNVKVTGRRNWSFTWKEETAGKEIWRIGVPDKSAGEYRHGYAVDESKTLHPEQYRI
YWGQYDFADDFPKGVNFEVGKSKEAEDLNYIHWSYFPAKGNHLRNESVYENVNNWTIAFD
LSKSQLSRAKTATFTVQIAGTKTANGNSKWSIIDHPYSNLPWTVNVNEGSYESTWWIPYF
RSGSCGVRSAVACQNIEHKFVFPTSKLKQGKNEFVLSLPFNASSIETAVLPDALYVQYDA
LRLEL

Enzyme Prediction     

EC	4.2.2.23:2

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
PL4	21	651	0	0.9968354430379747

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
199907	RGL4_N	1.01e-55	28	308	6	259	N-terminal catalytic domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold; the middle and C-terminal domains are both putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11.
199904	RGL4_M	1.06e-31	349	431	1	83	Middle domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold. Both the middle domain represented by this model and the C-terminal domain are putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11.
199905	RGL4_C	1.65e-25	449	664	1	161	C-terminal domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold. Both the middle and the C-terminal domain are putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11.
405387	fn3_3	6.69e-22	360	430	1	68	Polysaccharide lyase family 4, domain II. FnIII-like is domain II of rhamnogalacturonan lyase (RG-lyase). The full-length protein specifically recognizes and cleaves alpha-1,4 glycosidic bonds between l-rhamnose and d-galacturonic acids in the backbone of rhamnogalacturonan-I, a major component of the plant cell wall polysaccharide, pectin. This domain displays an immunoglobulin-like or more specifically Fibronectin-III type fold and shows highest structural similarity to the C-terminal beta-sandwich subdomain of the pro-hormone/propeptide processing enzyme carboxypeptidase gp180 from duck. It serves to assist in producing the deep pocket, with domain III, into which the substrate fits.
405384	CBM-like	1.10e-14	448	663	2	157	Polysaccharide lyase family 4, domain III. CBM-like is domain III of rhamnogalacturonan lyase (RG-lyase). The full-length protein specifically recognizes and cleaves alpha-1,4 glycosidic bonds between l-rhamnose and d-galacturonic acids in the backbone of rhamnogalacturonan-I, a major component of the plant cell wall polysaccharide, pectin. This domain possesses a jelly roll beta-sandwich fold structurally homologous to carbohydrate binding modules (CBMs), and it carries two sulfate ions and a hexa-coordinated calcium ion.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CEI70429.1|PL4_3	0.0	9	664	9	660
CEF87623.1|PL4_3	0.0	9	664	9	660
QKD61475.1|PL4_3	0.0	9	664	9	660
QPC62797.1|PL4_3	0.0	9	664	9	660
CZS85753.1|PL4_3	0.0	9	664	9	660

PDB Hits     

P170DRAFT_382675-t37_1-p1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q2U5P7|RGLC_ASPOR	0.0	9	657	9	654	Probable rhamnogalacturonate lyase C OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=rglC PE=3 SV=1
sp|Q5B5P1|RGLC_EMENI	0.0	15	657	15	650	Probable rhamnogalacturonate lyase C OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=rglC PE=3 SV=1
sp|Q5AZ85|RGLB_EMENI	2.06e-248	12	665	9	659	Rhamnogalacturonate lyase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=rglB PE=2 SV=2
sp|A5ABH4|RGLB_ASPNC	3.30e-241	15	651	13	648	Probable rhamnogalacturonate lyase B OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=rglB PE=3 SV=1
sp|B0XPA2|RGLB_ASPFC	3.60e-241	21	665	19	657	Probable rhamnogalacturonate lyase B OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=rglB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000246	0.999714	CS pos: 21-22. Pr: 0.9801

TMHMM  Annotations     

There is no transmembrane helices in P170DRAFT_382675-t37_1-p1.