CAZyme Information: P170DRAFT_356813-t37_1-p1

Basic Information

• Species: Aspergillus steynii
• Lineage: Ascomycota; Eurotiomycetes; ; Aspergillaceae; Aspergillus; Aspergillus steynii
• CAZyme ID: P170DRAFT_356813-t37_1-p1
• CAZy Family: AA7
• CAZyme Description: 1,3-beta-glucanosyltransferase gel3

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
536	MSFO01000004|CGC17	57195.39	4.6363

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AsteyniiIBT23096	13430	1392250	235	13195

• Gene Location: location=MSFO01000004:221566-223360(+)

Full Sequence      

MKLSLAVGATLLGAALADIDPIVIKGSKFFYSGNNTQFYIRGIAYQQEYSSNGTTDGSNT
YKDPLADATACERDVPIMQELNTNTIRVYAIDPTADHKKCMQLLSDAGIYVISDLSSPSQ
SIDRNDPRWEVSLYNRYTSVIDEMAQYNNTLGFFAGNEVSNNVKTTDASAFVKAAVRDMK
AYIKRKNYRSMGVGYATNDDSSIRVHMANYFNCESDEDSIDFWGYNIYSWCGDSTYQESG
YKDRTDEFRNYSVPVFFAEYGCNSVSPRKFTEVKALFGDKMNDVWSGGIVYMYFQEANDY
GLVSVVDSTSVSKLKDFTYYSSQIASATPSGTNKASYTPTNSALQSCPAVSTAWKATQTP
LPPTPNEELCNCMDASAGCVVKDSLSSSKYSDLFGVVCGYTDCDGLTANATTGKYGAYGM
CNSKQQLNFALNKYYAEQSSAASACSFGGSATTTSAASKTGSCKSLMSQAGTAGTGTVTS
QPTATAGSGSSEASASASGSTGAAPTTAGTAVAVGSFQLGLYAVLAVLTAATMVLL

Enzyme Prediction     

EC	2.4.1.-:3	2.4.1.-:33

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH72	17	326	4.8e-140	0.9871794871794872
CBM43	379	464	1.2e-20	0.9759036144578314

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
397351	Glyco_hydro_72	1.65e-161	11	327	1	314	Glucanosyltransferase. This is a family of glycosylphosphatidylinositol-anchored beta(1-3)glucanosyltransferases. The active site residues in the Aspergillus fumigatus example are the two glutamate residues at 160 and 261.
400371	X8	2.84e-30	379	452	3	76	X8 domain. The X8 domain domain contains at least 6 conserved cysteine residues that presumably form three disulphide bridges. The domain is found in an Olive pollen allergen as well as at the C-terminus of several families of glycosyl hydrolases. This domain may be involved in carbohydrate binding. This domain is characteristic of GPI-anchored domains.
197867	X8	3.04e-20	379	465	3	85	Possibly involved in carbohydrate binding. The X8 domain, which may be involved in carbohydrate binding, is found in an Olive pollen antigen as well as at the C terminus of family 17 glycosyl hydrolases. It contains 6 conserved cysteine residues which presumably form three disulfide bridges.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BAE66482.1|CBM43|GH72	1.06e-288	1	536	1	531
QMW48313.1|CBM43|GH72	8.66e-288	1	536	1	531
QRD92751.1|CBM43|GH72	1.23e-287	1	536	1	531
QMW36259.1|CBM43|GH72	1.23e-287	1	536	1	531
UDD65514.1|CBM43|GH72	1.23e-287	1	536	1	531

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2W62_A	8.79e-126	13	435	27	456	Saccharomyces cerevisiae Gas2p in complex with laminaripentaose [Saccharomyces cerevisiae],2W63_A Saccharomyces Cerevisiae Gas2p In Complex With Laminaritriose And Laminaritetraose [Saccharomyces cerevisiae],5O9O_A Crystal structure of ScGas2 in complex with compound 7. [Saccharomyces cerevisiae S288C],5O9P_A Crystal structure of Gas2 in complex with compound 10 [Saccharomyces cerevisiae S288C],5O9Q_A Crystal structure of ScGas2 in complex with compound 6 [Saccharomyces cerevisiae S288C],5O9R_A Crystal structure of ScGas2 in complex with compound 9 [Saccharomyces cerevisiae S288C],5O9Y_A Crystal structure of ScGas2 in complex with compound 11 [Saccharomyces cerevisiae S288C],5OA2_A Crystal structure of ScGas2 in complex with compound 8 [Saccharomyces cerevisiae S288C],5OA2_B Crystal structure of ScGas2 in complex with compound 8 [Saccharomyces cerevisiae S288C],5OA2_C Crystal structure of ScGas2 in complex with compound 8 [Saccharomyces cerevisiae S288C],5OA6_A Crystal structure of ScGas2 in complex with compound 12 [Saccharomyces cerevisiae S288C]
2W61_A	2.48e-125	13	435	27	456	Saccharomyces cerevisiae Gas2p apostructure (E176Q mutant) [Saccharomyces cerevisiae]
5FIH_A	2.48e-125	13	435	27	456	SACCHAROMYCES CEREVISIAE GAS2P (E176Q MUTANT) IN COMPLEX WITH LAMINARITETRAOSE AND LAMINARIPENTAOSE [Saccharomyces cerevisiae]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|D4ATC3|GAS1_ARTBC	2.98e-208	1	535	1	544	1,3-beta-glucanosyltransferase ARB_07487 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_07487 PE=1 SV=1
sp|P0C955|GEL3_ASPFU	3.34e-192	1	483	1	481	1,3-beta-glucanosyltransferase gel3 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=gel3 PE=3 SV=1
sp|B0XT09|GEL3_ASPFC	3.70e-192	1	483	1	481	1,3-beta-glucanosyltransferase gel3 OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=gel3 PE=1 SV=1
sp|P0C956|GEL4_ASPFU	3.81e-187	6	473	7	489	1,3-beta-glucanosyltransferase gel4 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=gel4 PE=3 SV=1
sp|B0XVI5|GEL4_ASPFC	3.81e-187	6	473	7	489	1,3-beta-glucanosyltransferase gel4 OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=gel4 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000253	0.999720	CS pos: 17-18. Pr: 0.9784

TMHMM  Annotations     

There is no transmembrane helices in P170DRAFT_356813-t37_1-p1.