CAZyme Information: P170DRAFT_349878-t37_1-p1

Basic Information

• Species: Aspergillus steynii
• Lineage: Ascomycota; Eurotiomycetes; ; Aspergillaceae; Aspergillus; Aspergillus steynii
• CAZyme ID: P170DRAFT_349878-t37_1-p1
• CAZy Family: AA4
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
557	MSFO01000002|CGC7	62727.87	6.0361

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AsteyniiIBT23096	13430	1392250	235	13195

• Gene Location: location=MSFO01000002:399274-401105(-)

Full Sequence      

MHISALSALGLFGAVAQAKPFLQRINDTAHVIGNDLWNVTIGRQYGVKLFYKDTDLVGDA
WGHYVSYNGAQSDLNWTSASIHRRGADYLDIKFTAREGDFHWVIFDQLAGAYQYFVNHDL
PTLGEFRSLWRLDNTSFPNGRTNIKDAPLPDLAEYPASEKVQDETWQRADGSFITKYDFS
AWVRELDFYGVYGERFGSWYINPGKDYYNGNHLKQELTVHRESATGDAVQLNMIHGTHFQ
VSAVDEFPDGKMWGPWLWYLNDGSKPDAEARAKKEAQAWPYKWFKDEAYHTRGSISGRLV
LSDGRPAAGASVFLGDNNPSKTALDMGSDYYYTGQTDSKGWFTFRNVRAGDYGLQAWSDG
GKLRDVMTTFSLNDVTVKKDKQTQLHKLEWKVSPRKKIFQVGDFDRKSLGFKHGGAPYQH
GLVAECPEDLVYTVGESETSDWCFGQSAKGTWSIKFPVDKKQANASAVLTVSLAGYSSGT
KSRILLNGDADIPIGNLTTLPNDPGLYRSATTAGEWRLFEFDVPEGKLREGWNTVDFEVT
ESTKWRGFMWDSIVLEA

Enzyme Prediction     

No EC number prediction in P170DRAFT_349878-t37_1-p1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
PL4	31	543	6.4e-246	0.9942196531791907

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
199907	RGL4_N	4.93e-59	21	268	1	264	N-terminal catalytic domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold; the middle and C-terminal domains are both putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11.
405384	CBM-like	5.67e-53	398	555	2	157	Polysaccharide lyase family 4, domain III. CBM-like is domain III of rhamnogalacturonan lyase (RG-lyase). The full-length protein specifically recognizes and cleaves alpha-1,4 glycosidic bonds between l-rhamnose and d-galacturonic acids in the backbone of rhamnogalacturonan-I, a major component of the plant cell wall polysaccharide, pectin. This domain possesses a jelly roll beta-sandwich fold structurally homologous to carbohydrate binding modules (CBMs), and it carries two sulfate ions and a hexa-coordinated calcium ion.
199905	RGL4_C	6.30e-38	399	556	1	161	C-terminal domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold. Both the middle and the C-terminal domain are putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11.
199904	RGL4_M	1.64e-20	292	388	2	92	Middle domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold. Both the middle domain represented by this model and the C-terminal domain are putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11.
404503	CarboxypepD_reg	1.30e-11	294	385	1	77	Carboxypeptidase regulatory-like domain.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AEO65538.1|PL4_5	1.49e-264	8	556	9	573
BCS19874.1|PL4_5	7.36e-255	20	556	29	571
UQC83548.1|PL4_5	4.24e-248	12	553	135	703
CBY02118.1|PL4_5	3.73e-245	19	556	30	569
CAE6996798.1|PL4_5	9.58e-237	19	556	26	563

PDB Hits     

P170DRAFT_349878-t37_1-p1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q8RJP2|RHIE_DICD3	2.08e-59	88	555	113	573	Rhamnogalacturonate lyase OS=Dickeya dadantii (strain 3937) OX=198628 GN=rhiE PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000284	0.999672	CS pos: 18-19. Pr: 0.9527

TMHMM  Annotations     

There is no transmembrane helices in P170DRAFT_349878-t37_1-p1.