CAZyme Information: P168DRAFT_275748-t37_1-p1

Basic Information

• Species: Aspergillus campestris
• Lineage: Ascomycota; Eurotiomycetes; ; Aspergillaceae; Aspergillus; Aspergillus campestris
• CAZyme ID: P168DRAFT_275748-t37_1-p1
• CAZy Family: GH17
• CAZyme Description: putative GPI mannosyltransferase 1

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
435	MSFM01000014|CGC4	47947.29	9.8309

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AcampestrisIBT28561	9929	1392248	173	9756

• Gene Location: location=MSFM01000014:245602-247076(-)

Full Sequence      

MASVFHRPSLIYGAAIALRLTLLIYGAWQDAHSAVKYTDIDYLVFTDAARYVARGESPYA
RDTYRYTPLLAWLLVPTASPGPFFFAFGKVLFALSDVLAGWLIARRLVSSYGMEPARALK
YASVWLLNPMVANISTRGSSEGLLCVLIVALLCAALSRRVVLAGVLLGLAVHLKIYPFVY
AASIVWWLDREREVSATATTTSPSGGSGSSGNGVAGKLIAFITPARIRLALAALTTFTAL
NITMYLLYGEPFLQHTYLHHLSRIDHRHNFSPYSSLLYLVAAGDVQTGWRFESLAFVPQL
VLSVVVIPLVLGKRCLAGTMLAQTFAFVTFNKVCTSQYFLWYLIFLPFYLPRSSLLRQPR
RGITVAVLWVVSQALWLQQGYNLEFLGQSSFVPGLFLASLGFFAVNCWILGIIVTDVGCL
SVQSEGRVKAKGRVE

Enzyme Prediction     

EC	2.4.1.-:14

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT50	134	413	1.7e-96	0.9923664122137404

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
178434	PLN02841	5.72e-86	14	414	14	415	GPI mannosyltransferase
252941	Mannosyl_trans	1.36e-74	134	414	1	259	Mannosyltransferase (PIG-M). PIG-M has a DXD motif. The DXD motif is found in many glycosyltransferases that utilize nucleotide sugars. It is thought that the motif is involved in the binding of a manganese ion that is required for association of the enzymes with nucleotide sugar substrates.
399599	PIG-U	2.44e-08	47	262	39	243	GPI transamidase subunit PIG-U. Many eukaryotic proteins are anchored to the cell surface via glycosylphosphatidylinositol (GPI), which is posttranslationally attached to the carboxyl-terminus by GPI transamidase. The mammalian GPI transamidase is a complex of at least four subunits, GPI8, GAA1, PIG-S, and PIG-T. PIG-U is thought to represent a fifth subunit in this complex and may be involved in the recognition of either the GPI attachment signal or the lipid portion of GPI.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
UDD65877.1|GT50	2.85e-215	1	419	1	415
BAE66105.1|GT50	7.06e-215	1	419	26	440
QRD92382.1|GT50	4.69e-214	1	419	1	415
QMW36573.1|GT50	6.05e-214	1	419	8	422
QMW48628.1|GT50	6.05e-214	1	419	8	422

PDB Hits     

P168DRAFT_275748-t37_1-p1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q2TXB8|GPI14_ASPOR	5.06e-216	1	419	1	415	GPI mannosyltransferase 1 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=gpi14 PE=3 SV=2
sp|Q4WAH2|GPI14_ASPFU	2.80e-213	1	418	1	422	GPI mannosyltransferase 1 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=gpi14 PE=3 SV=1
sp|Q5B7W0|GPI14_EMENI	4.32e-205	1	429	1	443	GPI mannosyltransferase 1 OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=gpi14 PE=3 SV=1
sp|Q7S4Z3|GPI14_NEUCR	5.44e-168	1	418	19	486	GPI mannosyltransferase 1 OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=gim-1 PE=3 SV=1
sp|Q4I073|GPI14_GIBZE	1.04e-159	5	424	7	415	GPI mannosyltransferase 1 OS=Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1) OX=229533 GN=GPI14 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000020	0.000028

TMHMM  Annotations     

Start	End
9	28
82	104
140	162
166	188
227	249
295	312
324	346
391	413