CAZyme Information: P168DRAFT_238591-t37_1-p1

Basic Information

• Species: Aspergillus campestris
• Lineage: Ascomycota; Eurotiomycetes; ; Aspergillaceae; Aspergillus; Aspergillus campestris
• CAZyme ID: P168DRAFT_238591-t37_1-p1
• CAZy Family: AA7
• CAZyme Description: beta-mannosidase mndA

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
929		104104.85	5.1145

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AcampestrisIBT28561	9929	1392248	173	9756

• Gene Location: location=MSFM01000008:511688-514711(-)

Full Sequence      

MRLGSGATQTILASSLSAGLGQHVTDLSDQIWTVSSPALNRTVPGYLPSHVHLDLLKAGV
IGIYNGLNDFHLRWIADANWTYTSDPIKGLYDDHASTWLVFDGLDTFATISFCGQPIAST
DNQFRQYSFDVSTVLKGCKDDPVLSINFGSAPRIVDAMAEDPSLEKWPDGVQLTHEYPNR
WWMRKEQSDFGWDWGPAFAPAGPWKPARIVQLDKPNSIYVLNTDLDIYRKGQINYLPPDQ
TQPWVVNASIDFVGNLPKNPSMAIEIQDGESGSVFTTQNLHNIVISGNSLTGVTVLDKVQ
PKLWWPSGLGDQNLYHITITVTGGHEEPVARVTKRTGFRTIFLNQRNITDAQLAQGVAPG
ANWHFEVNGREFYAKGSNIIPPDAFWPRVTEEKMARLFDAVIVGNQNMLRVWSSGAYLHD
FIYDLADERGILLWSEFEFSDALYPVHDAFLENVAAEVVYNVRRVNHHPSLALWAGGNEI
ESLMLPIVHGVDPKGYAKYVGEYEKLFISLILPLVYQNTRSISYTPSSTTEGYLNIDLSA
PVPMAERYDNKTAGSYYGDTDHYNYDTSVSFNYHTYPVGRFANEFGFHSMPSVQTWQQAV
APEDLHFNSSVVVLRNHHYVVGSLDTDNYANSSRGMGEMTMGVERYYPIPSNPDPIANFS
AWCHATQIFQADMYKSQIQFYRRGSGMPERQLGSLYWQLEDIWQAPSWAGIEYGGRWKVL
HYAARDIYQPVIVSPFWNYTTGDLSVYVTSDLWEAATGTVELKWLDLSGRRLASDVGTPS
SISFTVGALNTTKIYSTNIKDLSLPDQKNSILLLSLNGKGQLPNSEDIVSLDHSNHFTPV
FPKDLALVDPKVKVSFDAKTKTFTVEAQSGISLYTWLTHPEGVVGYFEENSFVLIPGQKK
TVGFVVQEDRTDGKWAQEVTVQSLWDQKA

Enzyme Prediction     

EC	3.2.1.25:4

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	47	801	2.8e-75	0.6582446808510638

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
225789	LacZ	4.26e-53	24	735	12	636	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
407625	Ig_mannosidase	1.14e-17	849	926	1	78	Ig-fold domain. This Ig-like fold domain is found in mannosidase enzymes.
407658	Mannosidase_ig	3.26e-12	742	844	1	91	Mannosidase Ig/CBM-like domain. This domain corresponds to domain 4 in the structure of Bacteroides thetaiotaomicron beta-mannosidase, BtMan2A. This domain has an Ig-like fold.
236673	ebgA	1.72e-07	101	479	132	449	cryptic beta-D-galactosidase subunit alpha; Reviewed
395572	Glyco_hydro_2	7.50e-07	239	339	13	106	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QMW39414.1|GH2	0.0	11	927	10	927
QMW27343.1|GH2	0.0	11	927	10	927
CAK96951.1|GH2|3.2.1.25	0.0	1	928	1	928
A2QWU9|GH2	0.0	1	928	1	928
BCS16350.1|GH2	0.0	1	928	1	929

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4CVU_A	0.0	24	926	30	938	Structure of Fungal beta-mannosidase from Glycoside Hydrolase Family 2 of Trichoderma harzianum [Trichoderma harzianum],4UOJ_A Chain A, BETA-MANNOSIDASE GH2 [Trichoderma harzianum],4UOJ_B Chain B, BETA-MANNOSIDASE GH2 [Trichoderma harzianum]
6DDT_A	6.87e-112	32	752	18	699	mouse beta-mannosidase (MANBA) [Mus musculus],6DDU_A mouse beta-mannosidase bound to beta-D-mannose (MANBA) [Mus musculus]
2VJX_A	2.04e-89	27	902	10	814	Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]
2JE8_A	2.11e-89	27	902	12	816	Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JE8_B Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
7OP6_A	2.16e-89	27	902	12	816	Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP6_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_A Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q4WMS9|MANBA_ASPFU	0.0	20	928	20	923	Beta-mannosidase A OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=mndA PE=3 SV=1
sp|O74168|MANBA_ASPAC	0.0	17	926	19	932	Beta-mannosidase A OS=Aspergillus aculeatus OX=5053 GN=mndA PE=1 SV=1
sp|Q2UN00|MANBA_ASPOR	0.0	11	927	10	910	Beta-mannosidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=mndA PE=3 SV=1
sp|Q0CI48|MANBA_ASPTN	0.0	1	927	1	928	Beta-mannosidase A OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=mndA PE=3 SV=2
sp|Q5BCI8|MANBA_EMENI	0.0	7	929	7	926	Beta-mannosidase A OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=mndA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.966561	0.033464

TMHMM  Annotations     

There is no transmembrane helices in P168DRAFT_238591-t37_1-p1.