CAZyme Information: OTA39271.1

Basic Information

• Species: Hortaea werneckii
• Lineage: Ascomycota; Dothideomycetes; ; Teratosphaeriaceae; Hortaea; Hortaea werneckii
• CAZyme ID: OTA39271.1
• CAZy Family: GT4
• CAZyme Description: Alpha,alpha-trehalase [Source:UniProtKB/TrEMBL;Acc:A0A1Z5TTM7]

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2222	MUNK01000004|CGC2	246529.67	4.3856

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_HwerneckiiEXF-2000	15649	1157616	29	15620

• Gene Location: location=MUNK01000004:369291-376970(-)

Full Sequence      

MSSISGGQDEGGNNEDVDADETTRTAESRTIEQGNSSSEDSDEDEEHYESLPEDSIVGDS
EAFLDETTVNAESEDGNIELDKQDDGERLDAADDPVDERGLDVQADGEPIGSQGQSPFLS
AADSGSIPDDLPSVQGSRVSSPGPLSLRGPRRTPQRTTSGALQPFEKRFESRFSTSPSPS
SRAGSPAFLTPHSRQISISSQFSQLSSQASSQGEGGSEAPIAPWEVIRWTKLRKLSGQAF
SESGKRNFGRPTTLAVSALIAVGTSKGLVLGFDYHQTLKVIIGQGTKATECGSVTALAIA
ADYSTIAAGHANGQIFTWDISKPARPFLQIPPLEKSVIQQSQHPDGHVSGQAILHIGFLG
TRHTALVSADAAGMAFSHLATRGLGPVTRTVKTTRLLGRYPTFDARVERGRKSSSVLAFS
PLPLGNVEQATDGMGLTALLTPYLLVIVSTTPIAQTQHKSPRPKEVMPHSTLSGCLAWFP
AVKLKSSNATAEKGNSDTKLVYCWSNVLTVLDVKVVENDDSNKPPSLEFHARSRWRADEA
IVAVQWLGRSVLGVITVSQRLLILEDPSLQVTDSLDLIQRHVYHQDLFSHHLQSVVDRLD
SDDPSLHGVVADAFYMSFKAYKGRTFLLGFNDLLVGVLSNWADRLMALMENGDHIGAIRL
GSEYYVRGGNKVTVGLPENDDARHEVVRERLLEMITASLNYSFSQQDDERDTRIRELADV
CFDACISMRETEYLFGRVFEIFEEAEEEDVFVLTLEPYVLDGDVSRLPPEVVKSAVAHFI
SENQAPRLEDLLCRLDPFSFDLDEITMLCRQHSLYDALIYVWGQGIGDFVTPLIDMMSLI
KILQAGDDDGDLSDNPFFESAIKVYPYLAYALTGRLYPRGDLLLDDDASKAKADLYGYVF
AGTPTAWPPGSQRYLYTVENPAEEPAFPYLVMLLEFDAAKFMTMLNEAFEDPFLNEADDD
TVPSNASEMNGVAGKMGYKMTRQHIISIMLDVMKQHEFDPDQVIYLDMFIARSLAKYPGQ
IVLSGALLDQVLQHLCHPPVEEVREDCQLSVEYLLSAYRPSDVSWLVDTLRQARFFHMLK
YVYRIERLYVELLETYFEDEVDQEGVFDAIGYCLRPNAGATAKQLEGVKRTMMQHIQELA
NISITSTARTFASSTADLLPSCIQALRDSYQQFMLLRCLLEPQLIRESHPPRSSGPSTID
LQTSYGELYVQLLCQHDPTHVAEYINVLPTSNLTLNNVLPAMEEAGVVDAAVVLLARDGM
VRDAMERLVVHLQTLQQALISMVEASAQAPDASSAETAALELVEDIEKYSKVGIWLCQGQ
SATAQRRPRPRMNVAWDVKEDDLDLDEYLWLNLVDAAVQVTKTTTGAVEHHEALPEDTGS
QAEAGDLAKIITALRSNTQQTFTALLAATATPSRPPSNDPRQPPPKQDNLSFLRVLRAFL
TRAATSAPSLADLRAVLSDIFSAYTFEQDVLSLANELLGSNVFTDIEEANSLRQRGWRPR
TQVCESCKRRVWGQGIGETVWDEWVLREQKRESEKARKLVERGGGEEARRLERGKAKAAV
DIVAERDDNQSEESRLLSFKSASALYTNGSVIAPCSSPLYCQGEILQQIELAQPYEDSKT
FVDLPTTRPLEEVIEAFNNLTKPIQNNTEIQTFLQDYFGEAGSELEPVPNESLSTNPTFL
NNVSSQVLRNFTDQVINIWPDLTRSYVGPPRCESASGGNASGCVSSFIPLNRTFVVAGGR
FREPYYWDSYWIIEGLLRTEGSFTEIALNIIENFLDLVDRFGFVPNGARQYYLNRSQPPL
LSQMVRAYVEFTNDTSILERALPTLEKEYMFWMENRTVSVRDGMNQTYTLNHYAVLNNQP
RPESFREDYITATNESYYAPTTGIIYPGQDLNETEQAELYANLASGAESGWDYSSRWLKN
PSDAANDNYFPLRSLNTKNIVPVDLNSILYANEITLANFYETVGGDDSESMVEEYQQRAA
NRSEAMAALMWDEEHFAYFDWNLTSNSRHIYEPLDSDATSSQIDDAPAGQQLLFSPAQYY
PFWTGAAPNWLKNNPYAVSRAYDRVAAQLEVAPGAIPATNLVTGEQWDEPNVWPPLQYIL
IQGLLNTPATFGTDDPWYRSTQNLAVDLAQRYVTSAFCTWRSTGGSTPQLPQLQGADRED
VGTIFEKYNQTSINAAGGGGEYEVVEGFGWSNGVLIWAADKFGRELKTPMCGNITAADIA
DE

Enzyme Prediction     

EC	3.2.1.28:1

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH37	1615	2200	1.8e-156	0.9918533604887984

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
215307	PLN02567	7.75e-138	1599	2203	12	545	alpha,alpha-trehalase
395961	Trehalase	3.14e-135	1615	2203	3	509	Trehalase. Trehalase (EC:3.2.1.28) is known to recycle trehalose to glucose. Trehalose is a physiological hallmark of heat-shock response in yeast and protects of proteins and membranes against a variety of stresses. This family is found in conjunction with pfam07492 in fungi.
224541	TreA	7.16e-87	1597	2207	53	557	Neutral trehalase [Carbohydrate transport and metabolism].
403885	Vps8	2.42e-82	750	949	1	194	Golgi CORVET complex core vacuolar protein 8. Vps8 is one of the Golgi complex components necessary for vacuolar sorting. Eukaryotic cells contain a highly dynamic endo-membrane system, in which individual organelles keep their identity despite continuous vesicle generation and fusion. Vesicles that bud from a donor membrane are targeted and delivered to each individual organelle, where they release their cargo after fusion with the acceptor membrane. Vps8 is the core component of the endosomal tethering complex CORVET (class C core vacuole/endosome tethering). Vps8 co-operates with Vps21-GTP to mediate endosomal clustering in a reaction that is dependent on Vps3. Vps8 is the only CORVET subunit that is enriched on late endosomes, suggesting that it is a marker for the maturation of late endosomes. Late endosomes form intralumenal vesicles, and the resulting multivesicular bodies fuse with the vacuole to release their cargoes.
237326	treA	6.81e-80	1576	2194	24	528	alpha,alpha-trehalase TreA.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
UKZ95675.1|GH37	4.34e-284	1584	2216	21	642
AEO70020.1|GH37	6.46e-284	1581	2217	18	643
UKZ71247.1|GH37	7.83e-282	1576	2216	12	642
ADX41482.1|GH37	7.92e-282	1582	2219	18	646
QRD06839.1|GH37	6.45e-279	1584	2218	21	646

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7E9U_A	5.11e-75	1617	2203	40	555	Chain A, Trehalase [Arabidopsis thaliana],7E9U_B Chain B, Trehalase [Arabidopsis thaliana]
7E9X_A	5.79e-74	1617	2203	40	555	Chain A, Trehalase [Arabidopsis thaliana],7E9X_B Chain B, Trehalase [Arabidopsis thaliana],7E9X_C Chain C, Trehalase [Arabidopsis thaliana],7E9X_D Chain D, Trehalase [Arabidopsis thaliana],7EAW_A Chain A, Trehalase [Arabidopsis thaliana],7EAW_B Chain B, Trehalase [Arabidopsis thaliana]
2JG0_A	2.25e-67	1603	2216	15	514	Family 37 trehalase from Escherichia coli in complex with 1- thiatrehazolin [Escherichia coli K-12],2JJB_A Family 37 trehalase from Escherichia coli in complex with casuarine-6- O-alpha-glucopyranose [Escherichia coli K-12],2JJB_B Family 37 trehalase from Escherichia coli in complex with casuarine-6- O-alpha-glucopyranose [Escherichia coli K-12],2JJB_C Family 37 trehalase from Escherichia coli in complex with casuarine-6- O-alpha-glucopyranose [Escherichia coli K-12],2JJB_D Family 37 trehalase from Escherichia coli in complex with casuarine-6- O-alpha-glucopyranose [Escherichia coli K-12],2WYN_A Structure of family 37 trehalase from Escherichia coli in complex with a casuarine-6-O-a-D-glucoside analogue [Escherichia coli K-12],2WYN_B Structure of family 37 trehalase from Escherichia coli in complex with a casuarine-6-O-a-D-glucoside analogue [Escherichia coli K-12],2WYN_C Structure of family 37 trehalase from Escherichia coli in complex with a casuarine-6-O-a-D-glucoside analogue [Escherichia coli K-12],2WYN_D Structure of family 37 trehalase from Escherichia coli in complex with a casuarine-6-O-a-D-glucoside analogue [Escherichia coli K-12]
5Z66_A	2.69e-66	1596	2207	45	544	Structure of periplasmic trehalase from Diamondback moth gut bacteria complexed with validoxylamine [Enterobacter cloacae],5Z6H_A Structure of periplasmic trehalase from Diamondback moth gut bacteria in the apo form [Enterobacter cloacae],5Z6H_B Structure of periplasmic trehalase from Diamondback moth gut bacteria in the apo form [Enterobacter cloacae]
2JF4_A	2.00e-63	1603	2216	15	514	Family 37 trehalase from Escherichia coli in complex with validoxylamine [Escherichia coli K-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q54QZ5|TREA_DICDI	3.80e-117	1585	2211	39	594	Trehalase OS=Dictyostelium discoideum OX=44689 GN=treh PE=3 SV=1
sp|A8J4S9|TREA_APIME	9.37e-109	1586	2206	32	572	Trehalase OS=Apis mellifera OX=7460 PE=1 SV=1
sp|Q9JLT2|TREA_MOUSE	1.82e-102	1594	2208	24	553	Trehalase OS=Mus musculus OX=10090 GN=Treh PE=1 SV=1
sp|P19813|TREA_RABIT	8.91e-102	1594	2208	27	555	Trehalase OS=Oryctolagus cuniculus OX=9986 GN=TREH PE=1 SV=1
sp|O43280|TREA_HUMAN	8.75e-101	1594	2208	27	556	Trehalase OS=Homo sapiens OX=9606 GN=TREH PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000019	0.000048

TMHMM  Annotations     

There is no transmembrane helices in OTA39271.1.