CAZyme Information: OTA38689.1

Basic Information

• Species: Hortaea werneckii
• Lineage: Ascomycota; Dothideomycetes; ; Teratosphaeriaceae; Hortaea; Hortaea werneckii
• CAZyme ID: OTA38689.1
• CAZy Family: GT32
• CAZyme Description: Beta-galactosidase [Source:UniProtKB/TrEMBL;Acc:A0A1Z5TRQ8]

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
990		108736.49	4.3463

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_HwerneckiiEXF-2000	15649	1157616	29	15620

• Gene Location: location=MUNK01000008:47919-51008(-)

Full Sequence      

MRIFSILSLLLLGALAALATDNGLTTAVTWDEYSLMVNGERVYIFSGEFAYERVPNPELW
RDILQKYKANGLNGVSIYFFWSYHSPREGVFDFRTPARDIQKLFDIAKEVGLYVIARPGP
YCNAETNGGGFALYTGDGSIGVPRSGDETYYEAWLPWIQEVDAIIAKNQITEGGNVIIIQ
MENELVEVRHDPDNTLVIYMEQLENATREAGVIVPTSHNEKGMRGQSWSTDYQDVGGAVN
IYGLDSYPGGLSCTNENTGFNVVRTYWQWFTNYSYTQPEYSPEFEAGWFSPWGADVFYDE
CSTELSPAFPDVYYKNMVGQQLTLLNLYMAFGGTNWGNLAAPVVYTSYDYSAPLRETREV
RNKFKQTKLLGLFTRVSKDVLKTEMESNGTGNAVSSSDIYSWVLRNGDNGAGFYTVQQQE
TPSRAVVSFDAYLNTSAGMVTAPGVELNGRQSKILTTDYHFGEHTLLYCSADIATYGIFG
DETVLVMYLNVGQKGEFAFKDAPRNMSFKTYGSANVSASAGSKNATGSEFTKYAYTQTAG
STVLRFSNGVSMYLLDQETAWNFWAPPTTLNPDVAPDEQVFALGPHTVRNVSLAGDVVSL
IGDNANKTSLEVFAGHKYSSIRWNGKELATKRTAYGSLKATAPGAEGRSIDLPQLSWVTA
DSLPEAQQDYDDSKWVVCNKNSSLSPETPLSLPVLFAADYKYYPGPYIYRGYFDGTKATS
ANATMQGGLAAGFTAWLNGKYVGGNTGDAEETSVSASLDFSGVSLNERNNVLTIVTDYTG
HDETSTDEGVKNARGLLGVTLAGGNSTLDFTQWKIQGNAGADANIDPVRGSLNEDGIHGT
RVGWHLPGFDPTGDAWSEGSPLDGLNSSGISWYITHFNLDIDEDLDVPLSIEFGAPENTF
ASVNLYVNGYQYGKYIPQIGPQTRFPFPPGVITNQGNNTIAVSLWAQTDAGARLSTASLF
AYEKYQTGFDFSQDWSELQPGWTSERLQYA

Enzyme Prediction     

EC	-	-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH35	35	370	1.4e-82	0.9869706840390879

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
396048	Glyco_hydro_35	3.57e-77	34	371	1	315	Glycosyl hydrolases family 35.
402180	BetaGal_dom2	8.83e-64	381	562	2	180	Beta-galactosidase, domain 2. This is the second domain of the five-domain beta-galactosidase enzyme that altogether catalyzes the hydrolysis of beta(1-3) and beta(1-4) galactosyl bonds in oligosaccharides as well as the inverse reaction of enzymatic condensation and trans-glycosylation. This domain is made up of 16 antiparallel beta-strands and an alpha-helix at its C-terminus. The fold of this domain appears to be unique. In addition, the last seven strands of the domain form a subdomain with an immunoglobulin-like (I-type Ig) fold in which the first strand is divided between the two beta-sheets. In penicillin spp this strand is interrupted by a 12-residue insertion which forms an additional edge-strand to the second beta-sheet of the sub-domain. The remainder of the second domain forms a series of beta-hairpins at its N-terminus, four strands of which are contiguous with part of the Ig-like sub-domain, forming in total a seven-stranded antiparallel beta-sheet. This domain is associated with family Glyco_hydro_35, pfam01301, which is N-terminal to it, but itself has no metazoan members.
198097	BetaGal_dom2	7.36e-43	379	563	1	182	Beta-galactosidase, domain 2. This is the second domain of the five-domain beta-galactosidase enzyme that altogether catalyses the hydrolysis of beta(1-3) and beta(1-4) galactosyl bonds in oligosaccharides as well as the inverse reaction of enzymatic condensation and trans-glycosylation. This domain is made up of 16 antiparallel beta-strands and an alpha-helix at its C terminus. The fold of this domain appears to be unique. In addition, the last seven strands of the domain form a subdomain with an immunoglobulin-like (I-type Ig) fold in which the first strand is divided between the two beta-sheets. In penicillin spp this strand is interrupted by a 12-residue insertion which forms an additional edge-strand to the second beta-sheet of the sub-domain. The remainder of the second domain forms a series of beta-hairpins at its N terminus, four strands of which are contiguous with part of the Ig-like sub-domain, forming in total a seven-stranded antiparallel beta-sheet. This domain is associated with family Glyco_hydro_35, which is N-terminal to it, but itself has no metazoan members.
404274	BetaGal_dom4_5	7.03e-30	838	950	1	111	Beta-galactosidase jelly roll domain. This domain is found in beta galactosidase enzymes. It has a jelly roll fold.
166698	PLN03059	3.12e-27	24	408	26	382	beta-galactosidase; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
UPP54608.1|GH35	0.0	14	990	15	1001
APA12177.1|GH35	0.0	1	990	1	984
BBN23886.1|GH35	0.0	1	990	1	982
CAP74292.1|GH35	0.0	1	990	1	982
CAE7174475.1|GH35	0.0	16	990	16	985

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1TG7_A	1.88e-169	28	959	6	943	Native structure of beta-galactosidase from Penicillium sp. [Penicillium sp.],1XC6_A Native Structure Of Beta-Galactosidase from Penicillium sp. in complex with Galactose [Penicillium sp.]
5IFP_A	1.26e-162	28	959	46	979	Structure Of Beta-galactosidase From Aspergillus Niger [Aspergillus niger CBS 513.88]
5IFT_A	3.47e-162	28	959	46	979	STRUCTURE OF E298Q-BETA-GALACTOSIDASE FROM ASPERGILLUS NIGER IN COMPLEX WITH 3-b-Galactopyranosyl glucose [Aspergillus niger CBS 513.88],5IHR_A Structure Of E298q-beta-galactosidase From Aspergillus Niger In Complex With Allolactose [Aspergillus niger CBS 513.88],5JUV_A STRUCTURE OF E298Q-BETA-GALACTOSIDASE FROM ASPERGILLUS NIGER IN COMPLEX WITH 6-b-Galactopyranosyl galactose [Aspergillus niger CBS 513.88],5MGC_A STRUCTURE OF E298Q-BETA-GALACTOSIDASE FROM ASPERGILLUS NIGER IN COMPLEX WITH 4-Galactosyl-lactose [Aspergillus niger CBS 513.88],5MGD_A STRUCTURE OF E298Q-BETA-GALACTOSIDASE FROM ASPERGILLUS NIGER IN COMPLEX WITH 6-Galactosyl-lactose [Aspergillus niger CBS 513.88]
3OG2_A	2.66e-157	24	968	22	984	Chain A, Beta-galactosidase [Trichoderma reesei],3OGR_A Chain A, Beta-galactosidase [Trichoderma reesei],3OGS_A Chain A, Beta-galactosidase [Trichoderma reesei],3OGV_A Chain A, Beta-galactosidase [Trichoderma reesei]
4IUG_A	1.15e-155	28	969	46	987	Crystal structure of beta-galactosidase from Aspergillus oryzae in complex with galactose [Aspergillus oryzae]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|B0Y752|BGALC_ASPFC	0.0	1	990	1	983	Probable beta-galactosidase C OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=lacC PE=3 SV=1
sp|A1DM65|BGALC_NEOFI	0.0	1	990	1	983	Probable beta-galactosidase C OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=lacC PE=3 SV=1
sp|A1CE56|BGALC_ASPCL	0.0	1	990	1	985	Probable beta-galactosidase C OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107) OX=344612 GN=lacC PE=3 SV=1
sp|B8N2I5|BGALC_ASPFN	0.0	1	990	1	984	Probable beta-galactosidase C OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=lacC PE=3 SV=1
sp|Q2UMD5|BGALC_ASPOR	0.0	1	990	1	984	Probable beta-galactosidase C OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=lacC PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000218	0.999786	CS pos: 19-20. Pr: 0.9759

TMHMM  Annotations     

There is no transmembrane helices in OTA38689.1.