CAZyme Information: OTA35373.1

Basic Information

• Species: Hortaea werneckii
• Lineage: Ascomycota; Dothideomycetes; ; Teratosphaeriaceae; Hortaea; Hortaea werneckii
• CAZyme ID: OTA35373.1
• CAZy Family: GH62
• CAZyme Description: 1,3-beta-glucanosyltransferase [Source:UniProtKB/TrEMBL;Acc:A0A1Z5TH77]

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1035	MUNK01000045|CGC1	113761.70	4.9075

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_HwerneckiiEXF-2000	15649	1157616	29	15620

• Gene Location: location=MUNK01000045:93203-98347(+)

Full Sequence      

MKAATIVAALVGTASANFLHLPKIAARATSSSSASSASGTSSGSLPTVTTKGNAFFAGDS
RFYIRGVDYQPGGSSEATDPLADESICSRDIPYFQKLGLNTTRVYTVDNSANHDKCMGLL
ADAGIYLALDVNSPKYSLNRAEPEVSYNSDYLQSVFATIDAFANYSNTLLFFSGNEVINS
DNSTTAAPYVKAVTRDMRQYIAERSYRSIPVGYSAADVSSNQYAMARYMDCGDDAMRSDF
YAINNYEWCDPSSYEESGWSTLVDQYSNYSLPLFMSEYGCITNTRKWTEAAELYKEEMTK
VFSGGIAYEYSQEGNGYGIYKIDGNSVSPVGNQPEWLGQELNSTSDPQNGGGYSTNGTKQ
DCPSQSDDWDTSLSRALPFPPSPAASRSLRCHRVPPEHPGGPKIILKYAGKDGTEEYDPI
HPPDTLDKFLDKSKHLGEVDMSTVEQEEKEEDPEEAERLERVKRMPILEQCYNLMDFEAV
ARRVMKKTAWAYYSSGADDEITMRENHGAFHKIWFRPRILQDVENIDLSTTMLGSKVSIP
FYVTATALGKLGNPEGEVVLTRGARKHNVIQMIPTLASCSFDEIVDAKEGDQVQWLQLYV
NKNREITERIVKHAESRGCKGLFITVDAPQLGRREKDMRSKFSDVGSNVQNTGGDNVDRS
QGAARAISSFIDPALSWKDIPWFKSITKMPIILKGVQRVEDVIRAVSVGCDGVVLSNHGG
RQLDFARSGVEVLAEVMPELRRLGLDNKIEVFIDGGIRRATDIIKALCLGAKGVGIGRPF
LYAMSAYGLPGVDRAMQLLKDEMEMNMRLIGCNSVDQLGPEFVDTRGLSMHSTEIQKAAQ
SASKFFNGKLDCLVNNAASTSGVGGPHLSDGQSFIDTWHEALETNLTGPMLMSQACLPML
EKFASQEGNSSSIIHISSTRAIQSELNNEPYSTTKAGILGLTQSMAVSLADRRIRVNTIL
PGWINVSNECKEADEKGEKWEDGLSKEDQAWHLTGRVGKVEDMLKAVEYLVHADFATGTE
VVVDGGVTRKMVYPE

Enzyme Prediction     

EC	2.4.1.-:26

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH72	44	327	9.1e-120	0.9358974358974359

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
239238	FCB2_FMN	0.0	476	821	2	344	Flavocytochrome b2 (FCB2) FMN-binding domain. FCB2 (AKA L-lactate:cytochrome c oxidoreductase) is a respiratory enzyme located in the intermembrane space of fungal mitochondria which catalyzes the oxidation of L-lactate to pyruvate. FCB2 also participates in a short electron-transport chain involving cytochrome c and cytochrome oxidase which ultimately directs the reducing equivalents gained from L-lactate oxidation to oxygen, yielding one molecule of ATP for every L-lactate molecule consumed. FCB2 is composed of 2 domains: a C-terminal flavin-binding domain, which includes the active site for lacate oxidation, and an N-terminal b2-cytochrome domain, required for efficient cytochrome c reduction. FCB2 is a homotetramer and contains two noncovalently bound cofactors, FMN and heme per subunit.
397351	Glyco_hydro_72	1.64e-158	45	345	9	315	Glucanosyltransferase. This is a family of glycosylphosphatidylinositol-anchored beta(1-3)glucanosyltransferases. The active site residues in the Aspergillus fumigatus example are the two glutamate residues at 160 and 261.
395850	FMN_dh	1.76e-152	481	825	1	350	FMN-dependent dehydrogenase.
239203	alpha_hydroxyacid_oxid_FMN	7.18e-130	476	820	2	299	Family of homologous FMN-dependent alpha-hydroxyacid oxidizing enzymes. This family occurs in both prokaryotes and eukaryotes. Members of this family include flavocytochrome b2 (FCB2), glycolate oxidase (GOX), lactate monooxygenase (LMO), mandelate dehydrogenase (MDH), and long chain hydroxyacid oxidase (LCHAO). In green plants, glycolate oxidase is one of the key enzymes in photorespiration where it oxidizes glycolate to glyoxylate. LMO catalyzes the oxidation of L-lactate to acetate and carbon dioxide. MDH oxidizes (S)-mandelate to phenylglyoxalate. It is an enzyme in the mandelate pathway that occurs in several strains of Pseudomonas which converts (R)-mandelate to benzoate.
224223	LldD	2.93e-105	474	823	1	348	FMN-dependent dehydrogenase, includes L-lactate dehydrogenase and type II isopentenyl diphosphate isomerase [Energy production and conversion, Lipid transport and metabolism, General function prediction only].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
UJO13392.1|GH72	2.64e-173	24	384	24	385
AQA29355.1|GH72	2.31e-151	14	370	10	358
UJO12414.1|GH72	2.31e-151	14	370	10	358
SMR44284.1|GH72	1.60e-149	41	383	33	374
SMY19438.1|GH72	1.24e-148	41	370	33	361

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1LTD_A	8.79e-152	393	835	33	485	The 2.6 Angstroms Refined Structure Of The Escherichia Coli Recombinant Saccharomyces Cerevisiae Flavocytochrome B2-Sulphite Complex [Saccharomyces cerevisiae],1LTD_B The 2.6 Angstroms Refined Structure Of The Escherichia Coli Recombinant Saccharomyces Cerevisiae Flavocytochrome B2-Sulphite Complex [Saccharomyces cerevisiae]
1FCB_A	1.04e-151	393	835	38	490	Molecular Structure Of Flavocytochrome B2 At 2.4 Angstroms Resolution [Saccharomyces cerevisiae],1FCB_B Molecular Structure Of Flavocytochrome B2 At 2.4 Angstroms Resolution [Saccharomyces cerevisiae],1KBI_A Crystallographic Study of the Recombinant Flavin-binding Domain of Baker's Yeast Flavocytochrome b2: Comparison with the Intact Wild-type Enzyme [Saccharomyces cerevisiae],1KBI_B Crystallographic Study of the Recombinant Flavin-binding Domain of Baker's Yeast Flavocytochrome b2: Comparison with the Intact Wild-type Enzyme [Saccharomyces cerevisiae]
1LCO_A	4.07e-151	393	835	38	490	X-RAY STRUCTURE OF TWO COMPLEXES OF THE Y143F FLAVOCYTOCHROME B2 MUTANT CRYSTALLIZED IN THE PRESENCE OF LACTATE OR PHENYL-LACTATE [Saccharomyces cerevisiae],1LCO_B X-RAY STRUCTURE OF TWO COMPLEXES OF THE Y143F FLAVOCYTOCHROME B2 MUTANT CRYSTALLIZED IN THE PRESENCE OF LACTATE OR PHENYL-LACTATE [Saccharomyces cerevisiae],1LDC_A X-RAY STRUCTURE OF TWO COMPLEXES OF THE Y143F FLAVOCYTOCHROME B2 MUTANT CRYSTALLIZED IN THE PRESENCE OF LACTATE OR PHENYL-LACTATE [Saccharomyces cerevisiae],1LDC_B X-RAY STRUCTURE OF TWO COMPLEXES OF THE Y143F FLAVOCYTOCHROME B2 MUTANT CRYSTALLIZED IN THE PRESENCE OF LACTATE OR PHENYL-LACTATE [Saccharomyces cerevisiae]
1SZE_A	5.73e-151	393	835	38	490	Chain A, Cytochrome b2, mitochondrial [Saccharomyces cerevisiae],1SZE_B Chain B, Cytochrome b2, mitochondrial [Saccharomyces cerevisiae]
2OZ0_A	1.59e-150	393	835	38	490	Chain A, Cytochrome b2 [Saccharomyces cerevisiae],2OZ0_B Chain B, Cytochrome b2 [Saccharomyces cerevisiae]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P09437|CYB2_WICAO	2.07e-182	396	837	113	551	L-lactate dehydrogenase (cytochrome) OS=Wickerhamomyces anomalus OX=4927 GN=CYB2 PE=1 SV=2
sp|P00175|CYB2_YEAST	7.22e-150	393	835	118	570	L-lactate dehydrogenase (cytochrome) OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=CYB2 PE=1 SV=1
sp|B0XT72|GEL1_ASPFC	6.94e-132	1	384	1	367	1,3-beta-glucanosyltransferase gel1 OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=gel1 PE=1 SV=1
sp|P0C7S9|GEL1_ASPFU	1.05e-130	1	384	1	367	1,3-beta-glucanosyltransferase gel1 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=gel1 PE=3 SV=1
sp|Q08193|GAS5_YEAST	3.06e-112	40	386	22	371	1,3-beta-glucanosyltransferase GAS5 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=GAS5 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000869	0.999091	CS pos: 27-28. Pr: 0.8594

TMHMM  Annotations     

There is no transmembrane helices in OTA35373.1.