dbCAN-seq: a database of CAZyme sequence and annotation

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CAZyme Information: OTA34069.1

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Basic Information help

Species

Hortaea werneckii

Lineage

Ascomycota; Dothideomycetes; ; Teratosphaeriaceae; Hortaea; Hortaea werneckii

CAZyme ID

OTA34069.1

CAZy Family

GH5

CAZyme Description

NmrA domain-containing protein [Source:UniProtKB/TrEMBL;Acc:A0A1Z5TDG5]

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
660	MUNK01000065\|CGC2	71845.73	4.6837

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_HwerneckiiEXF-2000	15649	1157616	29	15620

Gene Location

Full Sequence Download help

Enzyme Prediction help

No EC number prediction in OTA34069.1.

CAZyme Signature Domains help

Family	Start	End	Evalue	family coverage
GH28	320	649	1.2e-56	0.803076923076923

CDD Domains download full data without filtering help

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
187561	NmrA_like_SDR_a	4.73e-51	5	275	1	230	NmrA (a transcriptional regulator) and HSCARG (an NADPH sensor) like proteins, atypical (a) SDRs. NmrA and HSCARG like proteins. NmrA is a negative transcriptional regulator of various fungi, involved in the post-translational modulation of the GATA-type transcription factor AreA. NmrA lacks the canonical GXXGXXG NAD-binding motif and has altered residues at the catalytic triad, including a Met instead of the critical Tyr residue. NmrA may bind nucleotides but appears to lack any dehydrogenase activity. HSCARG has been identified as a putative NADP-sensing molecule, and redistributes and restructures in response to NADPH/NADP ratios. Like NmrA, it lacks most of the active site residues of the SDR family, but has an NAD(P)-binding motif similar to the extended SDR family, GXXGXXG. SDRs are a functionally diverse family of oxidoreductases that have a single domain with a structurally conserved Rossmann fold, an NAD(P)(H)-binding region, and a structurally diverse C-terminal region. Sequence identity between different SDR enzymes is typically in the 15-30% range; they catalyze a wide range of activities including the metabolism of steroids, cofactors, carbohydrates, lipids, aromatic compounds, and amino acids, and act in redox sensing. Atypical SDRs are distinct from classical SDRs. Classical SDRs have an TGXXX[AG]XG cofactor binding motif and a YXXXK active site motif, with the Tyr residue of the active site motif serving as a critical catalytic residue (Tyr-151, human 15-hydroxyprostaglandin dehydrogenase numbering). In addition to the Tyr and Lys, there is often an upstream Ser and/or an Asn, contributing to the active site; while substrate binding is in the C-terminal region, which determines specificity. The standard reaction mechanism is a 4-pro-S hydride transfer and proton relay involving the conserved Tyr and Lys, a water molecule stabilized by Asn, and nicotinamide. In addition to the Rossmann fold core region typical of all SDRs, extended SDRs have a less conserved C-terminal extension of approximately 100 amino acids, and typically have a TGXXGXXG cofactor binding motif. Complex (multidomain) SDRs such as ketoreductase domains of fatty acid synthase have a GGXGXXG NAD(P)-binding motif and an altered active site motif (YXXXN). Fungal type ketoacyl reductases have a TGXXXGX(1-2)G NAD(P)-binding motif.
398829	NmrA	5.69e-37	5	274	1	235	NmrA-like family. NmrA is a negative transcriptional regulator involved in the post-translational modification of the transcription factor AreA. NmrA is part of a system controlling nitrogen metabolite repression in fungi. This family only contains a few sequences as iteration results in significant matches to other Rossmann fold families.
395231	Glyco_hydro_28	2.52e-31	343	647	47	321	Glycosyl hydrolases family 28. Glycosyl hydrolase family 28 includes polygalacturonase EC:3.2.1.15 as well as rhamnogalacturonase A(RGase A), EC:3.2.1.-. These enzymes are important in cell wall metabolism.
177865	PLN02218	1.84e-27	347	626	156	401	polygalacturonase ADPG
215426	PLN02793	1.49e-24	347	643	143	404	Probable polygalacturonase

CAZyme Hits help

Hit ID	E-Value	Query Start	Query End	Hit Start
5.40e-173	319	614	94	389
5.72e-138	319	658	89	420
5.72e-138	319	658	89	420
2.28e-137	319	658	89	420
2.28e-137	319	658	89	420

PDB Hits download full data without filtering help

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
4.97e-14	344	617	92	329	Endo-Polygalacturonase Ii From Aspergillus Niger [Aspergillus niger],1CZF_B Endo-Polygalacturonase Ii From Aspergillus Niger [Aspergillus niger]
1.90e-13	355	623	109	351	Crystal structure of endo-xylogalacturonan hydrolase from Aspergillus tubingensis [Aspergillus tubingensis]
9.05e-13	3	286	5	256	Chain A, NmrA-like family domain-containing protein 1 [Homo sapiens],3E5M_B Chain B, NmrA-like family domain-containing protein 1 [Homo sapiens]
3.94e-12	3	286	6	257	Crystal structure of NmrA-like family domain containing protein 1 in complex with niflumic acid [Homo sapiens],2WMD_A Crystal structure of NmrA-like family domain containing protein 1 in complex with NADP and 2-(4-chloro-phenylamino)-nicotinic acid [Homo sapiens]
4.24e-12	3	286	12	263	Crystal structure of HSCARG from Homo sapiens in complex with NADP [Homo sapiens],2EXX_B Crystal structure of HSCARG from Homo sapiens in complex with NADP [Homo sapiens]

Swiss-Prot Hits download full data without filtering help

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
4.06e-138	319	658	89	420	Putative galacturan 1,4-alpha-galacturonidase C OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=rgxC PE=2 SV=1
1.44e-120	319	659	91	421	Putative galacturan 1,4-alpha-galacturonidase C OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=rgxC PE=3 SV=1
3.00e-49	319	660	92	435	Putative galacturan 1,4-alpha-galacturonidase A OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=rgxA PE=3 SV=2
3.00e-49	319	660	92	435	Putative galacturan 1,4-alpha-galacturonidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=rgxA PE=3 SV=1
2.77e-47	319	660	93	436	Putative galacturan 1,4-alpha-galacturonidase A OS=Aspergillus niger OX=5061 GN=rgxA PE=2 SV=1

SignalP and Lipop Annotations help

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000043	0.000000

TMHMM Annotations help

There is no transmembrane helices in OTA34069.1.