CAZyme Information: OTA33771.1

Basic Information

• Species: Hortaea werneckii
• Lineage: Ascomycota; Dothideomycetes; ; Teratosphaeriaceae; Hortaea; Hortaea werneckii
• CAZyme ID: OTA33771.1
• CAZy Family: GH45
• CAZyme Description: Alpha,alpha-trehalase [Source:UniProtKB/TrEMBL;Acc:A0A1Z5TD24]

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2181		241448.84	4.4001

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_HwerneckiiEXF-2000	15649	1157616	29	15620

• Gene Location: location=MUNK01000070:3380-11039(-)

Full Sequence      

MSSISGGQDEGGNNEDVDADETIRTAEARAIDQGGSSNEDSDEDEEHYESLPEDSIVGDS
EAFLDETTVNGESEDRNIEFDKQGDSERLDATDDPIDERGLDVEADGEPIGSQGQSPFLS
AADSGSIPDDLPSVQGSRVSSPGPLSLRGPRRTPQRTTSGALQPFDKRFESRFSTSPSPS
SRAGSPAFLTPHSRQISISSQFSQLSSQASSQGEGGNEAPIAPWEVIRWTKLRKLSGQAF
SESGKRNFGRPTTLAVSALIAVGTSKGLVLGFDYHQTLKVIIGQATKATECGSVTALAIA
ADYSTIAAGHANGQIFTWDISKPARPFLQIPPLEKSIVQQSQHPDGHVSGQAILHIGFLG
TRHTALVSADAAGMAFSHLATRGLGPVTRTVKTTRLLGRYPTFDARVERSRKSSSVLAFS
PLPLGNVEQATDGMGLTALLTPYLLVIVSTTPIAQTQHKSPRPKEVMPHSTLSGCLAWFP
AVKLKSSNATAEKGNSDTKLVYCWSNVLTVLDVKVVGNDDSNKPPSLEFHARSRWRADEA
IVAVQWLGRSVLGVITVSQRLLILEDPSLQVTDSLDLIQRHVYHQDLFSHQLQSVVDRLD
SDDPSLHGVVADAFYMSFKAYKGRTFLLGFNDLIVGVLSNWADRLMALMENGDHIGAIRL
GSEYYARGGNKVTVGLPENDDARHEVVQERLLEMITASLNYSFSQQDDERDTRIRELADV
CFDACISMHQTDYLFGRVFEIFEESEEEAVFVLTLEPYVLDGDVSRLPPEVVKSVVAHFI
SENQAPRLEDLLCRLDPLSFDLDEITMLCRQHSLYDALIYVWGQGIGDFVTPLIDMMSLI
KILQAGDDNGDLSDNPFFDSAIKVYPYLAYALTGRLYPRGDLLADDDASKAKADLYGYVF
AGTPTAWPPGSQRYLYTVENPAEEPAFPYLVMLLEFDAAKFMTMLNEAFEDPFLNEADED
TVPSNASEVNGVAGKVGYKMTRQHIISIMLDVMKQHEFDPDQIIYLDMFIARSLPKYPGQ
IVLSGALLDQVLQHLCHPPVEEVREDCQLSVEYLLSAYRPSDVSWLVNALRQARFFHMLK
YIYRTERLFVELLETYFEDEDDQEGVFDAIGYCLRPTAGATPKQLEGVKRTMMQHIQELA
NISIASTARTLVSFTADLLPSCIQALRDSYQQFMLLRCLLEPQLIRDGNPPRSSGPSTID
LQTSHGELYVQLLCQHDPTHVAEYINVLPTSDLTLNNVLSAMEEAGVVDAAVVLLARDGM
VRDAMERLVVHLQTLQQALISIVEASAQAPDASSAETAALELVGDIEKYTKVGIWLCQGQ
SATAQRRPRPRMNVAWDVKEDDLDLDEYLWLNLVDAAVQVTKNTTGAVEHHEEHPEDTGS
QTEAGDLAKIITALRSNTQQTFTALLAATATPSRPPSNDPRQPPPKQDNLSFLRVLRAFL
TRAATSAPSLADLRAVLSDIFSAYTFEQDVLSLASELLGSNVFADIAEATSLRQRGWRPR
TQVCETCKRRAWGQGIGETVWDEWVLREQKRDSEKARKLVERGGGEEARRLERGKAKANV
DTVAERDDTQSEESTHSKTFVDLPTTRPLEEVIAAFNNLTKPIQNNTELQTFLEDYFGEA
GSELEPVPNDSLSTNPTFLNNVCSQALRNFTDQVINIWPDLTRSYVGPPRCESASGGNAS
GCVSSFIPLNRTFVVAGGRFREPYYWDSYWIIEGLLRTEGSFTEIALNIIENFLDLVDRF
GFVPNGARQYYLNRSQPPLLSQMVRAYVEYTNDTSILERALPTLEKEYMFWMENRTVSVR
DGMNKTYTLNHYAVLNNQPRPESFREDYVTATNESYYAPSTGIIYPGQDLNETEQAELYA
NLASGAESGWDYSSRWLKNPSDAANDNYFPLRSLNTKNIVPVDLNSILYANEITLANFYE
TVGGDDSESMVEEYQQRAANRSEAMAALMWDEEQFAYFDWNLTSNSRHIYEPLDSDATSS
QIDDAPAGQQVLFSPAQYYPFWTGAAPTWLKNNPYAVSRAYDRVAAQLEVAPGAIPATNL
VTGEQWDEPNVWPPLQYILIQGLLNTPATFGTDDPSYQSIQNLAVELAQRYVTSAFCTWR
STGGSTPQLPQLQGADPEDVGTIFEKYNQTSINAAGGGGEYEVVEGFGWSNGVLIWAADK
FGRELKTPMCGNITAADIADE

Enzyme Prediction     

EC	3.2.1.28:1

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH37	1576	2159	7.4e-156	0.9877800407331976

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
215307	PLN02567	1.28e-135	1577	2162	30	545	alpha,alpha-trehalase
395961	Trehalase	1.01e-134	1577	2162	6	509	Trehalase. Trehalase (EC:3.2.1.28) is known to recycle trehalose to glucose. Trehalose is a physiological hallmark of heat-shock response in yeast and protects of proteins and membranes against a variety of stresses. This family is found in conjunction with pfam07492 in fungi.
224541	TreA	7.84e-84	1658	2166	138	557	Neutral trehalase [Carbohydrate transport and metabolism].
403885	Vps8	1.76e-83	750	949	1	194	Golgi CORVET complex core vacuolar protein 8. Vps8 is one of the Golgi complex components necessary for vacuolar sorting. Eukaryotic cells contain a highly dynamic endo-membrane system, in which individual organelles keep their identity despite continuous vesicle generation and fusion. Vesicles that bud from a donor membrane are targeted and delivered to each individual organelle, where they release their cargo after fusion with the acceptor membrane. Vps8 is the core component of the endosomal tethering complex CORVET (class C core vacuole/endosome tethering). Vps8 co-operates with Vps21-GTP to mediate endosomal clustering in a reaction that is dependent on Vps3. Vps8 is the only CORVET subunit that is enriched on late endosomes, suggesting that it is a marker for the maturation of late endosomes. Late endosomes form intralumenal vesicles, and the resulting multivesicular bodies fuse with the vacuole to release their cargoes.
183936	treA	1.82e-77	1570	2162	58	537	alpha,alpha-trehalase TreA.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AEO70020.1|GH37	1.59e-267	1575	2176	53	643
UKZ95675.1|GH37	5.81e-267	1577	2175	55	642
UKZ71247.1|GH37	2.04e-264	1577	2175	54	642
SMR59387.1|GH37	5.01e-264	1577	2170	50	632
SMY28604.1|GH37	7.03e-264	1577	2170	50	632

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7E9U_A	4.17e-74	1578	2162	42	555	Chain A, Trehalase [Arabidopsis thaliana],7E9U_B Chain B, Trehalase [Arabidopsis thaliana]
7E9X_A	4.72e-73	1578	2162	42	555	Chain A, Trehalase [Arabidopsis thaliana],7E9X_B Chain B, Trehalase [Arabidopsis thaliana],7E9X_C Chain C, Trehalase [Arabidopsis thaliana],7E9X_D Chain D, Trehalase [Arabidopsis thaliana],7EAW_A Chain A, Trehalase [Arabidopsis thaliana],7EAW_B Chain B, Trehalase [Arabidopsis thaliana]
2JG0_A	2.08e-65	1645	2175	83	514	Family 37 trehalase from Escherichia coli in complex with 1- thiatrehazolin [Escherichia coli K-12],2JJB_A Family 37 trehalase from Escherichia coli in complex with casuarine-6- O-alpha-glucopyranose [Escherichia coli K-12],2JJB_B Family 37 trehalase from Escherichia coli in complex with casuarine-6- O-alpha-glucopyranose [Escherichia coli K-12],2JJB_C Family 37 trehalase from Escherichia coli in complex with casuarine-6- O-alpha-glucopyranose [Escherichia coli K-12],2JJB_D Family 37 trehalase from Escherichia coli in complex with casuarine-6- O-alpha-glucopyranose [Escherichia coli K-12],2WYN_A Structure of family 37 trehalase from Escherichia coli in complex with a casuarine-6-O-a-D-glucoside analogue [Escherichia coli K-12],2WYN_B Structure of family 37 trehalase from Escherichia coli in complex with a casuarine-6-O-a-D-glucoside analogue [Escherichia coli K-12],2WYN_C Structure of family 37 trehalase from Escherichia coli in complex with a casuarine-6-O-a-D-glucoside analogue [Escherichia coli K-12],2WYN_D Structure of family 37 trehalase from Escherichia coli in complex with a casuarine-6-O-a-D-glucoside analogue [Escherichia coli K-12]
5Z66_A	1.68e-62	1645	2166	120	544	Structure of periplasmic trehalase from Diamondback moth gut bacteria complexed with validoxylamine [Enterobacter cloacae],5Z6H_A Structure of periplasmic trehalase from Diamondback moth gut bacteria in the apo form [Enterobacter cloacae],5Z6H_B Structure of periplasmic trehalase from Diamondback moth gut bacteria in the apo form [Enterobacter cloacae]
2JF4_A	1.82e-61	1645	2175	83	514	Family 37 trehalase from Escherichia coli in complex with validoxylamine [Escherichia coli K-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q54QZ5|TREA_DICDI	9.01e-107	1577	2170	72	594	Trehalase OS=Dictyostelium discoideum OX=44689 GN=treh PE=3 SV=1
sp|A8J4S9|TREA_APIME	6.63e-96	1577	2165	64	572	Trehalase OS=Apis mellifera OX=7460 PE=1 SV=1
sp|Q9W2M2|TREA_DROME	1.98e-90	1577	2166	76	582	Trehalase OS=Drosophila melanogaster OX=7227 GN=Treh PE=1 SV=1
sp|P19813|TREA_RABIT	7.60e-90	1578	2167	52	555	Trehalase OS=Oryctolagus cuniculus OX=9986 GN=TREH PE=1 SV=1
sp|O43280|TREA_HUMAN	1.35e-88	1578	2167	52	556	Trehalase OS=Homo sapiens OX=9606 GN=TREH PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.999961	0.000062

TMHMM  Annotations     

There is no transmembrane helices in OTA33771.1.