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CAZyme Information: OTA30605.1

You are here: Home > Sequence: OTA30605.1

Basic Information | Genomic context | Full Sequence | Enzyme annotations |  CAZy signature domains |  CDD domains | CAZyme hits | PDB hits | Swiss-Prot hits | SignalP and Lipop annotations | TMHMM annotations

Basic Information help

Species Hortaea werneckii
Lineage Ascomycota; Dothideomycetes; ; Teratosphaeriaceae; Hortaea; Hortaea werneckii
CAZyme ID OTA30605.1
CAZy Family GH18
CAZyme Description Alpha-galactosidase [Source:UniProtKB/TrEMBL;Acc:A0A1Z5T3K3]
CAZyme Property
Protein Length CGC Molecular Weight Isoelectric Point
536 57558.29 4.2304
Genome Property
Genome Version/Assembly ID Genes Strain NCBI Taxon ID Non Protein Coding Genes Protein Coding Genes
FungiDB-61_HwerneckiiEXF-2000 15649 1157616 29 15620
Gene Location

Full Sequence      Download help

Enzyme Prediction      help

EC 3.2.1.22:1 3.2.1.88:1 3.2.1.22:1 3.2.1.88:1

CAZyme Signature Domains help

Family Start End Evalue family coverage
GH27 123 348 4e-51 0.8777292576419214

CDD Domains      download full data without filtering help

Cdd ID Domain E-Value qStart qEnd sStart sEnd Domain Description
269893 GH27 1.56e-66 43 284 1 271
glycosyl hydrolase family 27 (GH27). GH27 enzymes occur in eukaryotes, prokaryotes, and archaea with a wide range of hydrolytic activities, including alpha-glucosidase (glucoamylase and sucrase-isomaltase), alpha-N-acetylgalactosaminidase, and 3-alpha-isomalto-dextranase. All GH27 enzymes cleave a terminal carbohydrate moiety from a substrate that varies considerably in size, depending on the enzyme, and may be either a starch or a glycoprotein. GH27 members are retaining enzymes that cleave their substrates via an acid/base-catalyzed, double-displacement mechanism involving a covalent glycosyl-enzyme intermediate. Two aspartic acid residues have been identified as the catalytic nucleophile and the acid/base, respectively.
166449 PLN02808 4.21e-46 40 364 29 376
alpha-galactosidase
177874 PLN02229 2.94e-43 41 364 61 410
alpha-galactosidase
178295 PLN02692 5.37e-43 40 364 53 401
alpha-galactosidase
271147 CBM35_galactosidase-like 2.66e-21 386 526 2 125
Carbohydrate Binding Module family 35 (CBM35); appended mainly to enzymes that bind alpha-D-galactose (CBM35-Gal), including glycoside hydrolase (GH) families GH27 and GH43. This family includes carbohydrate binding module family 35 (CBM35); these are non-catalytic carbohydrate binding domains that are appended mainly to enzymes that bind alpha-D-galactose (CBM35-Gal), including glycoside hydrolase (GH) families GH27 and GH43. Examples of proteins which contain CBM35s belonging to this family includes the CBM35 of an exo-beta-1,3-galactanase from Phanerochaete chrysosporium 9 (Pc1,3Gal43A) which is appended to a GH43 domain, and the CBM35 domain of two bifunctional proteins with beta-L-arabinopyranosidase/alpha-D-galactopyranosidase activities from Fusarium oxysporum 12S, Foap1 and Foap2 (Fo/AP1 and Fo/AP2), that are appended to GH27 domains. CBM35s are unique in that they display conserved specificity through extensive sequence similarity but divergent function through their appended catalytic modules. They are known to bind alpha-D-galactose (Gal), mannan (Man), xylan, glucuronic acid (GlcA), a beta-polymer of mannose, and possibly glucans, forming four subfamilies based on general ligand specificities (galacto, urono, manno, and gluco configurations). Some CBM35s bind their ligands in a calcium-dependent manner. In contrast to most CBMs that are generally rigid proteins, CBM35 undergoes significant conformational change upon ligand binding. GH43 includes beta-xylosidases and beta-xylanases, using aryl-glycosides as substrates, while family GH27 includes alpha-galactosidases, alpha-N-acetylgalactosaminidases, and isomaltodextranases.

CAZyme Hits      help

Hit ID E-Value Query Start Query End Hit Start Hit End
5.75e-182 40 528 26 561
2.49e-178 40 527 26 550
3.52e-178 40 527 26 550
4.03e-177 40 527 26 550
1.15e-176 40 527 26 550

PDB Hits      download full data without filtering help

Hit ID E-Value Query Start Query End Hit Start Hit End Description
7.06e-44 40 364 6 352
Chain A, alpha-galactosidase [Oryza sativa]
3.59e-35 40 346 6 336
Nicotiana benthamiana alpha-galactosidase [Nicotiana benthamiana]
3.61e-35 43 369 9 387
Crystal structure of alpha-galactosidase I from Mortierella vinacea [Umbelopsis vinacea]
1.32e-34 40 369 9 408
Chain A, alpha-galactosidase [Trichoderma reesei],1T0O_A Chain A, alpha-galactosidase [Trichoderma reesei]
9.45e-26 43 361 100 470
Crystal structure of a putative alpha-galactosidase (BF1418) from Bacteroides fragilis NCTC 9343 at 1.57 A resolution [Bacteroides fragilis NCTC 9343]

Swiss-Prot Hits      download full data without filtering help

Hit ID E-Value Query Start Query End Hit Start Hit End Description
3.78e-53 41 475 29 591
Probable alpha-galactosidase D OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=aglD PE=3 SV=2
3.78e-53 41 475 29 591
Probable alpha-galactosidase D OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=aglD PE=3 SV=2
3.21e-50 41 475 29 591
Probable alpha-galactosidase D OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=aglD PE=3 SV=1
2.55e-49 43 373 32 403
Probable alpha-galactosidase D OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=aglD PE=3 SV=2
1.88e-46 41 475 32 598
Probable alpha-galactosidase D OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=aglD PE=3 SV=2

SignalP and Lipop Annotations help

This protein is predicted as SP

Other SP_Sec_SPI CS Position
0.000281 0.999710 CS pos: 26-27. Pr: 0.9753

TMHMM  Annotations      help

There is no transmembrane helices in OTA30605.1.