CAZyme Information: ORE05353.1

Basic Information

• Species: Rhizopus microsporus
• Lineage: Mucoromycota; Mucoromycetes; ; Rhizopodaceae; Rhizopus; Rhizopus microsporus
• CAZyme ID: ORE05353.1
• CAZy Family: GH3
• CAZyme Description: chitin synthase 1

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
839	KV921947|CGC1	95060.16	7.8414

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_RmicrosporusATCC52814	11554	N/A	58	11496

• Gene Location: location=KV921947:77990-80821(+)

Full Sequence      

SEETSTFGDWLSSWFNISKEQESSTNLLQQQQQQGTNYARNQQTLSSLRSQKQQAEPSNK
QRENNRHKLTRSDTEKSYLDAPKSQQAGNGRKITRRLPLTNNHLVVDCPIPDRLLGALNF
SERDEFSQLRYTAATCEPDDFETEGYTLRQKIYNRETELFIVMTMYNEDEVLFTRTMHGV
MKNIAHLCSLKRSPMWGPDGWKKVVVCIVADGRKVVNKRVLNVLASMGVYQAGIAKNVVD
DKPVKAHIYEYTTQISIDPSMNIKGSDKGIVPVQVIFCLKEKNAKKINSHRWFFNAFGPI
LQPNVCILLDVGTRPGNSSIYQLWKVFNNNPLVGGACGEIRAMLGTACCQLLNPLVAAQN
FEYKMSNILDKPLESVFGYISVLPGAFSAYRYAALKNDANGHGPLEKYFLGEDLLHKDQS
GKQAGLFEANMYLAEDRILCFELVAKKNERWLLQYVNSAFGETDVPNQLPEFISQRRRWL
NGSFFAGVYGLIHFPKIWNSGHGFNRTLLLLIEGIYNVISLIFSWFAVGNFYIAFYFITK
SLADPSVDPFGNGWGERIFDFCRYVYASLLFIVFICSMGNRPQGSKHLFMACLVGFACIM
VYMLFCASWLIYKGIQLAAERHQWTLDTTTNFQIAMNDPGLRNMLISLSSTYGIYFVSSC
LHGQPFHMFTSFLPYLLLLPGYINILNIYAFCNTHDVSWGTKGDNTIAKDLGIVKVSDEK
EKGVKTAEVELPADQYDINCQYDAALFALDDRPNKMEENKSTLTKDDYQRAFRTHLVLAW
IACNAVLVIFITAPSYESIFVQSAGTTYMSVLLWVNCGLGIFRFIGSCIFLLLNIFTSG

Enzyme Prediction     

EC	2.4.1.16:26	2.4.1.16:26

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT2	165	327	3.3e-79	0.9938650306748467

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
396286	Chitin_synth_1	5.68e-117	165	327	1	163	Chitin synthase. This region is found commonly in chitin synthases classes I, II and III. Chitin a linear homopolymer of GlcNAc residues, it is an important component of the cell wall of fungi and is synthesized on the cytoplasmic surface of the cell membrane by membrane bound chitin synthases.
133033	Chitin_synth_C	9.25e-95	161	486	1	244	C-terminal domain of Chitin Synthase catalyzes the incorporation of GlcNAc from substrate UDP-GlcNAc into chitin. Chitin synthase, also called UDP-N-acetyl-D-glucosamine:chitin 4-beta-N-acetylglucosaminyltransferase, catalyzes the incorporation of GlcNAc from substrate UDP-GlcNAc into chitin, which is a linear homopolymer of GlcNAc residues formed by covalent beta-1,4 linkages. Chitin is an important component of the cell wall of fungi and bacteria and it is synthesized on the cytoplasmic surface of the cell membrane by membrane bound chitin synthases. Studies with fungi have revealed that most of them contain more than one chitin synthase gene. At least five subclasses of chitin synthases have been identified.
400626	Chitin_synth_1N	5.23e-35	96	164	1	70	Chitin synthase N-terminal. This is the N-terminal domain of Chitin synthase (pfam01644).
224136	BcsA	7.13e-29	263	619	102	413	Glycosyltransferase, catalytic subunit of cellulose synthase and poly-beta-1,6-N-acetylglucosamine synthase [Cell motility].
133045	CESA_like	5.37e-16	161	413	1	180	CESA_like is the cellulose synthase superfamily. The cellulose synthase (CESA) superfamily includes a wide variety of glycosyltransferase family 2 enzymes that share the common characteristic of catalyzing the elongation of polysaccharide chains. The members include cellulose synthase catalytic subunit, chitin synthase, glucan biosynthesis protein and other families of CESA-like proteins. Cellulose synthase catalyzes the polymerization reaction of cellulose, an aggregate of unbranched polymers of beta-1,4-linked glucose residues in plants, most algae, some bacteria and fungi, and even some animals. In bacteria, algae and lower eukaryotes, there is a second unrelated type of cellulose synthase (Type II), which produces acylated cellulose, a derivative of cellulose. Chitin synthase catalyzes the incorporation of GlcNAc from substrate UDP-GlcNAc into chitin, which is a linear homopolymer of beta-(1,4)-linked GlcNAc residues and Glucan Biosynthesis protein catalyzes the elongation of beta-1,2 polyglucose chains of Glucan.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CAA67797.1|GT2|2.4.1.16	0.0	2	839	7	852
CDS09401.1|GT2	0.0	91	838	63	804
CDS10052.1|GT2	6.31e-318	92	836	154	904
CDS03137.1|GT2	6.52e-316	78	838	82	833
CDS12882.1|GT2	3.35e-314	92	838	88	826

PDB Hits     

ORE05353.1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q12632|CHS1_MUCCL	0.0	2	839	7	852	Chitin synthase 1 OS=Mucor circinelloides f. lusitanicus OX=29924 GN=CHS1 PE=3 SV=1
sp|P87073|CHS1_PHYB8	1.08e-311	78	838	89	841	Chitin synthase 1 OS=Phycomyces blakesleeanus (strain ATCC 8743b / DSM 1359 / FGSC 10004 / NBRC 33097 / NRRL 1555) OX=763407 GN=chs1 PE=3 SV=1
sp|P30594|CHS1_RHIOL	8.43e-308	92	838	116	858	Chitin synthase 1 OS=Rhizopus oligosporus OX=4847 GN=CHS1 PE=3 SV=1
sp|P30595|CHS2_RHIOL	1.94e-306	78	838	106	858	Chitin synthase 2 OS=Rhizopus oligosporus OX=4847 GN=CHS2 PE=3 SV=1
sp|P30584|CHSA_EMENI	4.76e-273	78	827	206	939	Chitin synthase A OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=chsA PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000069	0.000000

TMHMM  Annotations     

Start	End
482	504
517	539
554	576
589	611
669	691
774	796
811	833