CAZyme Information: ORE04271.1

Basic Information

• Species: Rhizopus microsporus
• Lineage: Mucoromycota; Mucoromycetes; ; Rhizopodaceae; Rhizopus; Rhizopus microsporus
• CAZyme ID: ORE04271.1
• CAZy Family: GH18
• CAZyme Description: glycosyl transferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
884	KV921976|CGC1	100627.79	6.4432

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_RmicrosporusATCC52814	11554	N/A	58	11496

• Gene Location: location=KV921976:48028-50682(+)

Full Sequence      

MSTRPTAERRLSMPKLTRRLTNVVIHVDEDQNGNVEPVASESVPSHKSRHIKLEGDALKE
WKKYMTSKENDPESIKKDIVHHAVETLCRGPSNLDKLAIYQATALAVRDRILEEWNLTEE
IMREQNPKRCYYLSMEFLIGRALDNALLCLKAKDAYKKSVQDLGFSLEELLDQERDAALG
NGGLGRLAACYMDAAATLDYPTWGYGLRYQYGIFKQLINKEGYQTEMPDYWLDPTVNPWE
FPRNDVSYEVQFYGYVATKVNDKGESRMSWEGGQRVKAMAYDVPIPGFGTKGCGNIRLWS
SKSLNAFDFASFNEGDYDKSVADQKSAENLTSVLYPNDNHLVGKELRLKQEYFFVSASLQ
DIIHRFKRTNSAWKDFPGKVAVQLNDTHPTLAIPELQRILVDIEGLDWDAAWDIVTRVFA
FTNHTVLPEALERWSVPMMEHILPRHMQIIYDINLFFLQKVEKMHYGDRELLNRVSIIEE
SNPQQIRMAYLAVAGSHKVNGVAALHSDLVKSQLFPDFIRYYGPEKFINITNGITPRRWL
YQANPGLRDLITETLGSEKWVTHLDELKGLKKFANDEEFQKKWMQVKHDNKVRLAQWIKS
NLGINVNPNALFDIQVKRIHEYKRQFMNILGVIYRYKNIKLLSDEEKEQLTPRVVIFGGK
SAPGYYIAKMVIKLINTVAEVVNNDSTINDLLKVVYIPDYNVSLAEIITPASDISQHIST
AGTEASGTSNMKFVLNGGLILGTVDGANIEIRDEIGEDNIFMFGTLADKVADIRHHQKYH
GVLIDPNLQIVLQSIQSGEFGGPETASVFGPLINTLTYGGDYYLISADFEMYLDAQDHVD
AVYKDKKSWAKKSILCTAGMGFFSADRAVKEYADKIWNLKPVSI

Enzyme Prediction     

EC	2.4.1.1:158

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT35	160	879	8.1e-275	0.9955489614243324

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
223136	GlgP	0.0	91	881	25	750	Glucan phosphorylase [Carbohydrate transport and metabolism].
237881	PRK14985	0.0	132	880	63	797	maltodextrin phosphorylase; Provisional
273967	P_ylase	0.0	79	879	1	794	glycogen/starch/alpha-glucan phosphorylases. This family consists of phosphorylases. Members use phosphate to break alpha 1,4 linkages between pairs of glucose residues at the end of long glucose polymers, releasing alpha-D-glucose 1-phosphate. The nomenclature convention is to preface the name according to the natural substrate, as in glycogen phosphorylase, starch phosphorylase, maltodextrin phosphorylase, etc. Name differences among these substrates reflect differences in patterns of branching with alpha 1,6 linkages. Members include allosterically regulated and unregulated forms. A related family, TIGR02094, contains examples known to act well on particularly small alpha 1,4 glucans, as may be found after import from exogenous sources. [Energy metabolism, Biosynthesis and degradation of polysaccharides]
340853	GT35_Glycogen_Phosphorylase	0.0	76	879	1	795	glycogen phosphorylase and similar proteins. This is a family of oligosaccharide phosphorylases. It includes yeast and mammalian glycogen phosphorylases, plant starch/glucan phosphorylase, as well as the maltodextrin phosphorylases of bacteria. The members of this family catalyze the breakdown of oligosaccharides into glucose-1-phosphate units. They are important allosteric enzymes in carbohydrate metabolism. The allosteric control mechanisms of yeast and mammalian members of this family are different from that of bacterial members. The members of this family belong to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility.
395272	Phosphorylase	0.0	160	879	1	661	Carbohydrate phosphorylase. The members of this family catalyze the formation of glucose 1-phosphate from one of the following polyglucoses; glycogen, starch, glucan or maltodextrin.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
EGX46445.1|GT35	0.0	61	884	55	873
APA13713.1|GT35	0.0	61	883	60	877
BCS26059.1|GT35	0.0	61	884	59	877
QNQ01362.1|GT35	0.0	46	881	56	883
ATZ57832.1|GT35	0.0	61	883	60	877

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1YGP_A	0.0	14	883	1	879	Phosphorylated Form Of Yeast Glycogen Phosphorylase With Phosphate Bound In The Active Site. [Saccharomyces cerevisiae],1YGP_B Phosphorylated Form Of Yeast Glycogen Phosphorylase With Phosphate Bound In The Active Site. [Saccharomyces cerevisiae]
4BQE_A	2.06e-281	70	884	58	873	Arabidopsis thaliana Cytosolic Alpha-1,4-glucan Phosphorylase (PHS2) [Arabidopsis thaliana],4BQE_B Arabidopsis thaliana Cytosolic Alpha-1,4-glucan Phosphorylase (PHS2) [Arabidopsis thaliana],4BQF_A Arabidopsis thaliana cytosolic alpha-1,4-glucan phosphorylase (PHS2) in complex with acarbose [Arabidopsis thaliana],4BQF_B Arabidopsis thaliana cytosolic alpha-1,4-glucan phosphorylase (PHS2) in complex with acarbose [Arabidopsis thaliana],4BQI_A ARABIDOPSIS THALIANA cytosolic alpha-1,4-glucan phosphorylase (PHS2) in complex with maltotriose [Arabidopsis thaliana],4BQI_B ARABIDOPSIS THALIANA cytosolic alpha-1,4-glucan phosphorylase (PHS2) in complex with maltotriose [Arabidopsis thaliana]
5LR8_A	1.11e-262	71	882	28	923	Structure of plastidial phosphorylase Pho1 from Barley [Hordeum vulgare subsp. vulgare],5LR8_B Structure of plastidial phosphorylase Pho1 from Barley [Hordeum vulgare subsp. vulgare],5LRA_A Plastidial phosphorylase PhoI from barley in complex with maltotetraose [Hordeum vulgare subsp. vulgare],5LRA_B Plastidial phosphorylase PhoI from barley in complex with maltotetraose [Hordeum vulgare subsp. vulgare],5LRB_A Plastidial phosphorylase from Barley in complex with acarbose [Hordeum vulgare subsp. vulgare],5LRB_B Plastidial phosphorylase from Barley in complex with acarbose [Hordeum vulgare subsp. vulgare]
3CEH_A	3.89e-254	75	881	5	807	Human liver glycogen phosphorylase (tense state) in complex with the allosteric inhibitor AVE5688 [Homo sapiens],3CEH_B Human liver glycogen phosphorylase (tense state) in complex with the allosteric inhibitor AVE5688 [Homo sapiens],3CEJ_A Human glycogen phosphorylase (tense state) in complex with the allosteric inhibitor AVE2865 [Homo sapiens],3CEJ_B Human glycogen phosphorylase (tense state) in complex with the allosteric inhibitor AVE2865 [Homo sapiens],3CEM_A Human glycogen phosphorylase (tense state) in complex with the allosteric inhibitor AVE9423 [Homo sapiens],3CEM_B Human glycogen phosphorylase (tense state) in complex with the allosteric inhibitor AVE9423 [Homo sapiens]
1FA9_A	1.35e-253	75	881	27	829	HUMAN LIVER GLYCOGEN PHOSPHORYLASE A COMPLEXED WITH AMP [Homo sapiens]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q00766|PHS1_DICDI	0.0	69	884	38	845	Glycogen phosphorylase 1 OS=Dictyostelium discoideum OX=44689 GN=glpV PE=1 SV=3
sp|P06738|PHSG_YEAST	0.0	13	883	23	902	Glycogen phosphorylase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=GPH1 PE=1 SV=4
sp|P34114|PHS2_DICDI	1.18e-299	44	880	79	908	Glycogen phosphorylase 2 OS=Dictyostelium discoideum OX=44689 GN=glpD PE=1 SV=2
sp|Q9SD76|PHS2_ARATH	3.45e-281	70	884	25	840	Alpha-glucan phosphorylase 2, cytosolic OS=Arabidopsis thaliana OX=3702 GN=PHS2 PE=1 SV=1
sp|P53537|PHSH_VICFA	7.15e-281	72	884	30	841	Alpha-glucan phosphorylase, H isozyme OS=Vicia faba OX=3906 PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000052	0.000000

TMHMM  Annotations     

There is no transmembrane helices in ORE04271.1.