CAZyme Information: OJD18611.1

Basic Information

• Species: Emergomyces pasteurianus
• Lineage: Ascomycota; Eurotiomycetes; ; Ajellomycetaceae; Emergomyces; Emergomyces pasteurianus
• CAZyme ID: OJD18611.1
• CAZy Family: GT24
• CAZyme Description: Dolichyl-phosphate-mannose--protein mannosyltransferase [Source:UniProtKB/TrEMBL;Acc:A0A1J9QEI7]

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
789	LGRN01000030|CGC1	89362.81	8.8815

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_EpasteurianusEp9510	9078	1447872	128	8950

• Gene Location: location=LGRN01000030:68223-70843(-)

Full Sequence      

MSSPPPSLRQRGGKKIAVVKEPQADSPSSKLKQAAPKPVAKPAAQWDYRVALVIVTLLAF
VTRFANISYPDEVVFDEVHFGKFASHYLQRAYFFDVHPPFGKLLFAFMGWLVGFNGEFLF
ENIGDSYIENKVPYVALRAMPAIQGALTIPVIFLIMWESGYSLPACILSAGLVLFDNAHV
AEDRLILLDATLVLTMALSILCYIRFSKFRHEPFGRKWWKWLLLTGVSLSCVISTKYVGA
FTFFTIGAAVMIDLWDLLNVNRPEGAISISQFTKHFAARAVALIIVPFFFYLFWFQVHFA
ILNRSGTGDDFMSPAFQETLSDNAMTSNSVEIMYNDQLTIRHKETKTYLHSHPNYYPLRY
EDGRVSSQGQQVTGYPFNDTNNHWMILPAAPFPADDALGHPVKNGDIVQLRHIQTDTFLL
THDVASPLYPTNQEFTTVSRELADGERHNDTLFEIKIENGNADQEFRTMSSLFKLVHVPT
RVAMWTHTTPLPDWAFKQAEINGNKMVQQASNIWFVEDIPSLDPESERLKKEPRVPKQLP
FLVKYIELQRAMFFHNNALTSSHPYASEPFQWPFLLRGVSFWTKNDTREQIYFLGNPIGW
WIASGLLAVFAGIIGADQLSLRRGIDALEEIWGPGTRARLYNSTGFFFIGWAAHYFPFYL
MGRQRFLHHYLPAHLASALVAGALIEFIFNIDPIHANGINHRIANAPGSNVMANSTGFTN
PITGRRYKKPRSQLGRQSHLATWAAVVAILGATIYGFYFFAPLTYGRPSLDVPGVLARKW
LSYDLHFAK

Enzyme Prediction     

EC	2.4.1.109:8

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT39	55	299	3.4e-71	0.9910313901345291

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
224839	PMT1	0.0	47	789	22	699	Dolichyl-phosphate-mannose--protein O-mannosyl transferase [Posttranslational modification, protein turnover, chaperones].
396786	PMT	4.59e-78	53	303	1	245	Dolichyl-phosphate-mannose-protein mannosyltransferase. This is a family of Dolichyl-phosphate-mannose-protein mannosyltransferase proteins EC:2.4.1.109. These proteins are responsible for O-linked glycosylation of proteins, they catalyze the reaction:- Dolichyl phosphate D-mannose + protein <=> dolichyl phosphate + O-D-mannosyl-protein. Also in this family is Drosophila rotated abdomen protein which is a putative mannosyltransferase. This family appears to be distantly related to pfam02516 (A Bateman pers. obs.). This family also contains sequences from ArnTs (4-amino-4-deoxy-L-arabinose lipid A transferase). They catalyze the addition of 4-amino-4-deoxy-l-arabinose (l-Ara4N) to the lipid A moiety of the lipopolysaccharide. This is a critical modification enabling bacteria (e.g. Escherichia coli and Salmonella typhimurium) to resist killing by antimicrobial peptides such as polymyxins. Members such as undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase are predicted to have 12 trans-membrane regions. The N-terminal portion of these proteins is hypothesized to have a conserved glycosylation activity which is shared between distantly related oligosaccharyltransferases ArnT and PglB families.
406576	PMT_4TMC	7.34e-78	544	782	2	198	C-terminal four TMM region of protein-O-mannosyltransferase. PMT_4TMC is the C-terminal four membrane-pass region of protein-O-mannosyltransferases and similar enzymes.
397103	MIR	9.13e-19	350	497	13	164	MIR domain. The MIR (protein mannosyltransferase, IP3R and RyR) domain is a domain that may have a ligand transferase function.
197746	MIR	1.12e-08	334	389	6	57	Domain in ryanodine and inositol trisphosphate receptors and protein O-mannosyltransferases.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QSS55406.1|GT39	0.0	1	789	1	776
QSS72813.1|GT39	0.0	1	789	1	776
QSS65660.1|GT39	0.0	1	789	1	776
BCS03793.1|GT39	0.0	2	789	3	775
BCS15407.1|GT39	0.0	2	789	3	775

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6P25_A	5.97e-102	61	768	62	712	Structure of S. cerevisiae protein O-mannosyltransferase Pmt1-Pmt2 complex bound to the sugar donor and a peptide acceptor [Saccharomyces cerevisiae W303],6P2R_A Structure of S. cerevisiae protein O-mannosyltransferase Pmt1-Pmt2 complex bound to the sugar donor [Saccharomyces cerevisiae W303]
6P25_B	1.50e-92	7	673	22	681	Structure of S. cerevisiae protein O-mannosyltransferase Pmt1-Pmt2 complex bound to the sugar donor and a peptide acceptor [Saccharomyces cerevisiae W303],6P2R_B Structure of S. cerevisiae protein O-mannosyltransferase Pmt1-Pmt2 complex bound to the sugar donor [Saccharomyces cerevisiae W303]
1T9F_A	1.40e-06	349	441	24	108	Structural genomics of Caenorhabditis elegans: Structure of a protein with unknown function [Caenorhabditis elegans]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q59X23|PMT4_CANAL	2.33e-250	7	789	4	755	Dolichyl-phosphate-mannose--protein mannosyltransferase 4 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=PMT4 PE=2 SV=2
sp|O42933|PMT4_SCHPO	9.05e-243	48	782	58	768	Dolichyl-phosphate-mannose--protein mannosyltransferase 4 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=ogm4 PE=3 SV=1
sp|P46971|PMT4_YEAST	3.29e-236	25	789	20	762	Dolichyl-phosphate-mannose--protein mannosyltransferase 4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=PMT4 PE=1 SV=1
sp|Q8R2R1|POMT1_MOUSE	1.31e-118	47	789	36	746	Protein O-mannosyl-transferase 1 OS=Mus musculus OX=10090 GN=Pomt1 PE=1 SV=1
sp|Q99PR0|POMT1_RAT	3.96e-117	47	789	36	746	Protein O-mannosyl-transferase 1 OS=Rattus norvegicus OX=10116 GN=Pomt1 PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.999945	0.000059

TMHMM  Annotations     

Start	End
50	67
92	114
135	157
185	207
228	250
280	302
592	614
638	660
667	689
739	761