CAZyme Information: OJD09798.1

Basic Information

• Species: Blastomyces percursus
• Lineage: Ascomycota; Eurotiomycetes; ; Ajellomycetaceae; Blastomyces; Blastomyces percursus
• CAZyme ID: OJD09798.1
• CAZy Family: AA11
• CAZyme Description: Chitinase [Source:UniProtKB/TrEMBL;Acc:A0A1J9P0Q7]

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
373		41779.68	4.7203

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_BpercursusEI222	10384	N/A	99	10285

• Gene Location: location=LGTZ01003342:269-1572(+)

Full Sequence      

MDIPGNYITHILYSFANVKPDSGEVYLTDTWADLEKRYPSDSWEEPGENVYGCIKQLYLL
KKHYRHLKTLLSIGGWTYSSNFPAPASTETGRQTFARTAVQLLADLGFDGIDIDWEYPED
GAQAKDFVLLLQTTREALDNYSAQHAQGRRLLLTVAVPCGEKNYKKLLMSEMDEYLDFWN
LMSYDFAGSWDQKAGHMANMFPSGNVPSSTPFNADQAITAYVAGGVHPKKIVFGLPLYGR
AFEQTDGPGHPFQGVGEGSWEPGVWDYKDLPRPGSEEVNDDDLMASWSYDRNAKKMISYD
TPAIAAEKADYIRQRGMGGGMWWELSGDVPVGSERSLIATTVNGLGGVRNLDHSENLLDY
PASRYENLRKGFR

Enzyme Prediction     

EC	3.2.1.14:25

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH18	4	330	1.8e-73	0.847972972972973

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
119365	GH18_chitinase	8.11e-122	2	328	20	322	The GH18 (glycosyl hydrolases, family 18) type II chitinases hydrolyze chitin, an abundant polymer of N-acetylglucosamine and have been identified in bacteria, fungi, insects, plants, viruses, and protozoan parasites. The structure of this domain is an eight-stranded alpha/beta barrel with a pronounced active-site cleft at the C-terminal end of the beta-barrel.
214753	Glyco_18	8.12e-115	2	328	20	334	Glyco_18 domain.
395573	Glyco_hydro_18	2.09e-86	3	328	18	307	Glycosyl hydrolases family 18.
225862	ChiA	1.09e-79	2	347	59	438	Chitinase, GH18 family [Carbohydrate transport and metabolism].
119351	GH18_chitolectin_chitotriosidase	9.92e-72	2	325	22	338	This conserved domain family includes a large number of catalytically inactive chitinase-like lectins (chitolectins) including YKL-39, YKL-40 (HCGP39), YM1, oviductin, and AMCase (acidic mammalian chitinase), as well as catalytically active chitotriosidases. The conserved domain is an eight-stranded alpha/beta barrel fold belonging to the family 18 glycosyl hydrolases. The fold has a pronounced active-site cleft at the C-terminal end of the beta-barrel. The chitolectins lack a key active site glutamate (the proton donor required for hydrolytic activity) but retain highly conserved residues involved in oligosaccharide binding. Chitotriosidase is a chitinolytic enzyme expressed in maturing macrophages, which suggests that it plays a part in antimicrobial defense. Chitotriosidase hydrolyzes chitotriose, as well as colloidal chitin to yield chitobiose and is therefore considered an exochitinase. Chitotriosidase occurs in two major forms, the large form being converted to the small form by either RNA or post-translational processing. Although the small form, containing the chitinase domain alone, is sufficient for the chitinolytic activity, the additional C-terminal chitin-binding domain of the large form plays a role in processing colloidal chitin. The chitotriosidase gene is nonessential in humans, as about 35% of the population are heterozygous and 6% homozygous for an inactivated form of the gene. HCGP39 is a 39-kDa human cartilage glycoprotein thought to play a role in connective tissue remodeling and defense against pathogens.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QSS59041.1|CBM50|GH18	3.71e-236	1	373	117	489
QSS54072.1|CBM50|GH18	8.68e-235	1	373	117	489
QSS75284.1|CBM50|GH18	1.75e-234	1	373	117	489
AAP80452.1|CBM50|GH18	1.23e-197	2	372	112	481
BCS01115.1|CBM50|GH18	8.77e-182	2	371	146	514

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1D2K_A	3.01e-175	2	371	23	389	Chain A, CHITINASE 1 [Coccidioides immitis],1LL4_A Chain A, Chitinase 1 [Coccidioides immitis],1LL4_B Chain B, Chitinase 1 [Coccidioides immitis],1LL4_C Chain C, Chitinase 1 [Coccidioides immitis],1LL4_D Chain D, Chitinase 1 [Coccidioides immitis]
1LL7_A	8.59e-175	2	371	23	389	Chain A, CHITINASE 1 [Coccidioides immitis],1LL7_B Chain B, CHITINASE 1 [Coccidioides immitis]
1LL6_A	1.73e-174	2	371	23	389	Chain A, CHITINASE 1 [Coccidioides immitis],1LL6_B Chain B, CHITINASE 1 [Coccidioides immitis],1LL6_C Chain C, CHITINASE 1 [Coccidioides immitis],1LL6_D Chain D, CHITINASE 1 [Coccidioides immitis]
6IGY_A	3.67e-166	2	372	36	403	Crystal structure of Aspergillus niger chitinase B [Aspergillus niger]
1WNO_A	3.89e-162	2	373	25	394	Crystal structure of a native chitinase from Aspergillus fumigatus YJ-407 [Aspergillus fumigatus],1WNO_B Crystal structure of a native chitinase from Aspergillus fumigatus YJ-407 [Aspergillus fumigatus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q1E3R8|CHI1_COCIM	5.31e-174	2	371	58	424	Endochitinase 1 OS=Coccidioides immitis (strain RS) OX=246410 GN=CTS1 PE=3 SV=1
sp|P0CB51|CHI1_COCPS	5.31e-174	2	371	58	424	Endochitinase 1 OS=Coccidioides posadasii (strain RMSCC 757 / Silveira) OX=443226 GN=CTS1 PE=1 SV=1
sp|C5P230|CHI1_COCP7	2.15e-173	2	371	58	424	Endochitinase 1 OS=Coccidioides posadasii (strain C735) OX=222929 GN=CTS1 PE=3 SV=1
sp|Q873X9|CHIB1_ASPFM	7.47e-161	2	373	63	432	Endochitinase B1 OS=Neosartorya fumigata OX=746128 GN=chiB1 PE=1 SV=1
sp|E9QRF2|CHIB1_ASPFU	7.47e-161	2	373	63	432	Endochitinase B1 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=chiB1 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000051	0.000001

TMHMM  Annotations     

There is no transmembrane helices in OJD09798.1.