CAZyme Information: ODM22860.1

Basic Information

• Species: Aspergillus cristatus
• Lineage: Ascomycota; Eurotiomycetes; ; Aspergillaceae; Aspergillus; Aspergillus cristatus
• CAZyme ID: ODM22860.1
• CAZy Family: GT32
• CAZyme Description: 1,3-beta-glucanosyltransferase gel4

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
538	JXNT01000001|CGC8	57744.25	4.5425

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AcristatusGZAAS20.1005	10344	N/A	0	10344

• Gene Location: location=JXNT01000001:1203120-1204787(+)

Full Sequence      

MKLTNVVAGATLFASTVLADLDPIVIKGSKFFYKSNDTQFYIKGVAYQQEYSGPKSDTDN
FKDPLADADACKRDIPYLEKLGANTIRTYAIDPKADHSECMKLLQDAGIYVISDLSSPSE
SIVRNDPKWDADLYSRYVSVIDEMSQYSNVIGFFAGNEVSNQANNTDASAFVKAAVRDMK
QYIKAKNYRTMGVGYATNDDADIREKMANYFNCDNADDSIDFWGYNIYSWCGDSNYQKSG
YKTRTEEFKDYSVPVFFAEYGCNAVQPRKFTEVEALYGKQMNDVWSGGIVYMYFQEENDY
GLVSVDGDKVSTLDDFNNLSSQLAKVSATGTKKSDYKPTNSALQSCPAVNSAWRAAATPL
PPSPNKDLCTCMMDSLECTVKGKVDGEKVGELFGTVCGYGVCDGVSANATTGDYGAYSVC
TPQEQLAYAMNLYYKQQKSKGSGASACDFNGAATTKTSSSPSGTCSALLKEAGSSGTGTV
TSSPTGNANQLSASGSASASGSEGAAPMVAPGAVRVGVWQFGAYLATAVVAGAGMILL

Enzyme Prediction     

EC	2.4.1.-:3	2.4.1.-:33

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH72	19	326	1.2e-140	0.9903846153846154
CBM43	378	466	7.8e-19	0.9759036144578314

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
397351	Glyco_hydro_72	1.37e-172	19	327	7	315	Glucanosyltransferase. This is a family of glycosylphosphatidylinositol-anchored beta(1-3)glucanosyltransferases. The active site residues in the Aspergillus fumigatus example are the two glutamate residues at 160 and 261.
400371	X8	2.40e-27	376	454	1	76	X8 domain. The X8 domain domain contains at least 6 conserved cysteine residues that presumably form three disulphide bridges. The domain is found in an Olive pollen allergen as well as at the C-terminus of several families of glycosyl hydrolases. This domain may be involved in carbohydrate binding. This domain is characteristic of GPI-anchored domains.
197867	X8	5.84e-19	378	467	3	85	Possibly involved in carbohydrate binding. The X8 domain, which may be involved in carbohydrate binding, is found in an Olive pollen antigen as well as at the C terminus of family 17 glycosyl hydrolases. It contains 6 conserved cysteine residues which presumably form three disulfide bridges.
225344	BglC	0.002	15	246	7	290	Aryl-phospho-beta-D-glucosidase BglC, GH1 family [Carbohydrate transport and metabolism].
395098	Cellulase	0.007	40	287	8	265	Cellulase (glycosyl hydrolase family 5).

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BCR83038.1|CBM43|GH72	0.0	1	538	1	538
EAL89081.1|CBM43|GH72	3.47e-276	1	538	1	541
QRD89178.1|CBM43|GH72	8.89e-276	1	538	1	538
QMW24910.1|CBM43|GH72	8.89e-276	1	538	1	538
QMW36910.1|CBM43|GH72	8.89e-276	1	538	1	538

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2W62_A	2.94e-121	24	434	36	456	Saccharomyces cerevisiae Gas2p in complex with laminaripentaose [Saccharomyces cerevisiae],2W63_A Saccharomyces Cerevisiae Gas2p In Complex With Laminaritriose And Laminaritetraose [Saccharomyces cerevisiae],5O9O_A Crystal structure of ScGas2 in complex with compound 7. [Saccharomyces cerevisiae S288C],5O9P_A Crystal structure of Gas2 in complex with compound 10 [Saccharomyces cerevisiae S288C],5O9Q_A Crystal structure of ScGas2 in complex with compound 6 [Saccharomyces cerevisiae S288C],5O9R_A Crystal structure of ScGas2 in complex with compound 9 [Saccharomyces cerevisiae S288C],5O9Y_A Crystal structure of ScGas2 in complex with compound 11 [Saccharomyces cerevisiae S288C],5OA2_A Crystal structure of ScGas2 in complex with compound 8 [Saccharomyces cerevisiae S288C],5OA2_B Crystal structure of ScGas2 in complex with compound 8 [Saccharomyces cerevisiae S288C],5OA2_C Crystal structure of ScGas2 in complex with compound 8 [Saccharomyces cerevisiae S288C],5OA6_A Crystal structure of ScGas2 in complex with compound 12 [Saccharomyces cerevisiae S288C]
2W61_A	8.26e-121	24	434	36	456	Saccharomyces cerevisiae Gas2p apostructure (E176Q mutant) [Saccharomyces cerevisiae]
5FIH_A	8.26e-121	24	434	36	456	SACCHAROMYCES CEREVISIAE GAS2P (E176Q MUTANT) IN COMPLEX WITH LAMINARITETRAOSE AND LAMINARIPENTAOSE [Saccharomyces cerevisiae]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|D4ATC3|GAS1_ARTBC	2.61e-229	1	538	1	545	1,3-beta-glucanosyltransferase ARB_07487 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_07487 PE=1 SV=1
sp|B0XVI5|GEL4_ASPFC	1.67e-201	1	538	1	548	1,3-beta-glucanosyltransferase gel4 OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=gel4 PE=1 SV=1
sp|P0C956|GEL4_ASPFU	1.67e-201	1	538	1	548	1,3-beta-glucanosyltransferase gel4 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=gel4 PE=3 SV=1
sp|P0C955|GEL3_ASPFU	2.04e-186	5	480	4	476	1,3-beta-glucanosyltransferase gel3 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=gel3 PE=3 SV=1
sp|B0XT09|GEL3_ASPFC	2.26e-186	5	480	4	476	1,3-beta-glucanosyltransferase gel3 OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=gel3 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000314	0.999663	CS pos: 19-20. Pr: 0.9785

TMHMM  Annotations     

There is no transmembrane helices in ODM22860.1.