CAZyme Information: OAX84549.1

Basic Information

• Species: Emergomyces africanus
• Lineage: Ascomycota; Eurotiomycetes; ; Ajellomycetaceae; Emergomyces; Emergomyces africanus
• CAZyme ID: OAX84549.1
• CAZy Family: GT4
• CAZyme Description: Beta-mannosidase A [Source:UniProtKB/TrEMBL;Acc:A0A1B7P6E1]

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
883		101159.31	5.7659

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_EafricanusCBS136260	8860	N/A	91	8769

• Gene Location: location=LGUA01000068:35684-38480(+)

Full Sequence      

MAERSVIPLKNGWVFKQADDEKAEFLPVSQFPTNVHLDLMHHGLIPDPFIGKNELDVQWV
GEKSWVYKTSFDAPALRDGDKAVLAFDGLDTHATVLLNDTKILETENMFIPERVDVTELL
RESPGSNVPNVLKITFHSTYLIGKKIKEQYPEHHWSCWNGDCSRLAVRKAPYHYGWDWGP
TLITCGPWRPVNLEVYSARISDLYFRQNVNKSLKQAALVVTAEVEGDAKNVTFYISIGGE
EKGSETVDIKDGVATVTFHIQNPELWYPSTYGKQPLYTLTASLSSNGVNLDSISKRVGLR
RAELIQRKMDEVEGTSFFFEVNNIPVFCGGSNWIPADNFIPRISPQKYYDWVKLMVEANQ
VMVRVWGGGIYEEQAFYDACDELGLLVWQDFLFACGIYPVFPSFLETVKREAIANVKILR
HHPSIVIWAGNNEDYLYAEDEKLNWDPSDNDPNSWLNSEFPARYIYEKILVDVTKDLIPD
TYYHFGSPWGGKDTADPTIGDIHQWNVWHGTQQKYQDYDKLSGRFVSEFGMQGFPDMKTI
NGYLAGGKTDPDRHPQSSTVDFHNKAGGHERRIALYLAENIRYNVEPFEQYVYATQLIQA
ECLASAYRLWKRQWKGPGREYCAGALVWQINDCWPVTSWAIVDYHSRPKQAFYAMKRELA
PLTVGLKRTSHTEYADKYTNAYYKTTHKIEIWATNLTVEQRCVCVMFKAWDVVTGRETYS
KKLHDSFVLKENRSTEISEFKIPPQDKKCESRNRDEANRTVVAAYLIENGEQVARCVNWP
EPLKHTHLQIPKELQVSLSEDGKQVGLSAEVPVKGVVIYADEDEVVFEDNCVDLVPGETV
MCELSAVEQHNNKKLIFIVTHQRNTRQVSRDNLIHSIESQRSL

Enzyme Prediction     

EC	3.2.1.25:3

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	3	769	2.1e-96	0.7047872340425532

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
225789	LacZ	9.22e-83	1	654	7	625	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
395572	Glyco_hydro_2	3.24e-13	198	300	1	106	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
236657	PRK10150	1.57e-11	67	327	69	293	beta-D-glucuronidase; Provisional
407658	Mannosidase_ig	1.90e-11	688	785	2	91	Mannosidase Ig/CBM-like domain. This domain corresponds to domain 4 in the structure of Bacteroides thetaiotaomicron beta-mannosidase, BtMan2A. This domain has an Ig-like fold.
407625	Ig_mannosidase	4.39e-05	793	840	5	52	Ig-fold domain. This Ig-like fold domain is found in mannosidase enzymes.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QSS71278.1|GH2	0.0	1	855	1	852
QSS62028.1|GH2	0.0	1	855	1	852
UQC87618.1|GH2	0.0	1	840	26	855
ATZ49691.1|GH2	0.0	1	840	24	858
CCD56258.2|GH2	0.0	1	840	1	835

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5N6U_A	2.32e-142	1	667	21	661	Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_B Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_C Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_D Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12]
7OP6_A	4.11e-128	6	875	10	849	Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP6_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_A Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482]
2VJX_A	7.47e-128	6	865	8	837	Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]
2JE8_A	7.86e-128	6	865	10	839	Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JE8_B Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
2WBK_A	1.07e-127	6	875	8	847	Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482],2WBK_B Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|I2C092|MANBB_THETO	0.0	1	845	1	839	Beta-mannosidase B OS=Thermothelomyces thermophilus OX=78579 GN=man9 PE=1 SV=1
sp|Q0CCA0|MANBB_ASPTN	2.78e-275	9	838	9	820	Beta-mannosidase B OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=mndB PE=3 SV=2
sp|A1D911|MANBB_NEOFI	3.13e-272	1	838	1	822	Beta-mannosidase B OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=mndB PE=3 SV=1
sp|A1CGA8|MANBB_ASPCL	1.78e-271	1	838	1	822	Beta-mannosidase B OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107) OX=344612 GN=mndB PE=3 SV=1
sp|Q2TXB7|MANBB_ASPOR	1.58e-269	1	838	1	821	Beta-mannosidase B OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=mndB PE=3 SV=3

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000092	0.000000

TMHMM  Annotations     

There is no transmembrane helices in OAX84549.1.