CAZyme Information: OAG23253.1

Basic Information

• Species: Alternaria alternata
• Lineage: Ascomycota; Dothideomycetes; ; Pleosporaceae; Alternaria; Alternaria alternata
• CAZyme ID: OAG23253.1
• CAZy Family: GH72
• CAZyme Description: concanavalin A-like lectin/glucanase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
375		41282.75	4.3720

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AalternataSRC1lrK2f	13577	N/A	111	13466

• Gene Location: location=KV441473:818678-819934(-)

Full Sequence      

MFSRGTWGASPWVLLLAMARFSNAACECGYSVNATTDPSYAVFTELMENDFLHTSTDDLK
EVGWQPQEYNVTSKNARGPFGKSMELTNIVTNPFKKGWSGEADHGGDAGLELWVRGDHQD
GLVSGAELVTMRNDSLLGSFRVGMKLSNSSGTCGAFFFYHNNSQEIDMEFLSHQFNNSQG
AVNLVLQSPESVTHGYAAQGTNGFEVMALPFRPDEMFHEYRFDWTPNRVAFYVDGKLLHE
MTENIPTESGGIFLNHWSNGDPSWSAGPPDQDTVMTISYMKAYFNSTDTERSQNDYRDRC
PTYDPAKVCAIPNQMSPPDQSQGVDAAKTYFFSTDGDDMTPGQTTFTGSASSMFSAPSLS
LAIPLLVTLLSLTLG

Enzyme Prediction     

No EC number prediction in OAG23253.1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH16	100	283	3e-80	0.96875

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
185683	Glyco_hydrolase_16	6.15e-49	106	283	36	210	glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.
185684	GH16_lichenase	5.94e-21	92	279	23	205	lichenase, member of glycosyl hydrolase family 16. Lichenase, also known as 1,3-1,4-beta-glucanase, is a member of glycosyl hydrolase family 16, that specifically cleaves 1,4-beta-D-glucosidic bonds in mixed-linked beta glucans that also contain 1,3-beta-D-glucosidic linkages. Natural substrates of beta-glucanase are beta-glucans from grain endosperm cell walls or lichenan from the Islandic moss, Cetraria islandica. This protein is found not only in bacteria but also in anaerobic fungi. This domain includes two seven-stranded antiparallel beta-sheets that are adjacent to one another forming a compact, jellyroll beta-sandwich structure.
395585	Glyco_hydro_16	4.82e-12	124	274	21	164	Glycosyl hydrolases family 16.
225182	BglS	5.64e-09	138	287	119	262	Beta-glucanase, GH16 family [Carbohydrate transport and metabolism].
185685	GH16_XET	1.53e-07	136	236	47	142	Xyloglucan endotransglycosylase, member of glycosyl hydrolase family 16. Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls via a transglycosylation mechanism. Xyloglucan is a soluble hemicellulose with a backbone of beta-1,4-linked glucose units, partially substituted with alpha-1,6-linked xylopyranose branches. It binds noncovalently to cellulose, cross-linking the adjacent cellulose microfibrils, giving it a key structural role as a matrix polymer. Therefore, XET plays an important role in all plant processes that require cell wall remodeling.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CAE7022543.1|GH16_23	1.28e-210	1	371	1	374
CBX96484.1|GH16_23	6.51e-205	1	371	1	370
QRD03541.1|GH16_23	1.41e-197	1	375	1	375
QKX60960.1|GH16_23	2.75e-89	9	352	14	350
APA11129.1|GH16_23	7.13e-87	15	365	13	369

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1CPN_A	7.14e-14	138	292	15	175	NATIVE-LIKE IN VIVO FOLDING OF A CIRCULARLY PERMUTED JELLYROLL PROTEIN SHOWN BY CRYSTAL STRUCTURE ANALYSIS [Paenibacillus macerans]
1MAC_A	1.42e-13	138	264	71	190	Crystal Structure And Site-Directed Mutagenesis Of Bacillus Macerans Endo-1,3-1,4-Beta-Glucanase [Paenibacillus macerans],1MAC_B Crystal Structure And Site-Directed Mutagenesis Of Bacillus Macerans Endo-1,3-1,4-Beta-Glucanase [Paenibacillus macerans]
1CPM_A	1.47e-13	138	264	15	134	NATIVE-LIKE IN VIVO FOLDING OF A CIRCULARLY PERMUTED JELLYROLL PROTEIN SHOWN BY CRYSTAL STRUCTURE ANALYSIS [Paenibacillus macerans]
1BYH_A	1.47e-13	138	264	73	192	MOLECULAR AND ACTIVE-SITE STRUCTURE OF A BACILLUS (1-3,1-4)-BETA-GLUCANASE [synthetic construct],1GLH_A Cation Binding To A Bacillus (1,3-1,4)-Beta-Glucanase. Geometry, Affinity And Effect On Protein Stability [Paenibacillus macerans],2AYH_A Crystal And Molecular Structure At 1.6 Angstroms Resolution Of The Hybrid Bacillus Endo-1,3-1,4-Beta-D-Glucan 4- Glucanohydrolase H(A16-M) [hybrid]
1AXK_A	4.55e-13	138	294	15	164	Engineered Bacillus Bifunctional Enzyme Gluxyn-1 [Bacillus subtilis],1AXK_B Engineered Bacillus Bifunctional Enzyme Gluxyn-1 [Bacillus subtilis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P45797|GUB_PAEPO	4.39e-13	138	264	97	216	Beta-glucanase OS=Paenibacillus polymyxa OX=1406 GN=gluB PE=3 SV=1
sp|P23904|GUB_PAEMA	1.08e-12	138	264	96	215	Beta-glucanase OS=Paenibacillus macerans OX=44252 PE=1 SV=2
sp|P04957|GUB_BACSU	9.83e-12	138	264	101	220	Beta-glucanase OS=Bacillus subtilis (strain 168) OX=224308 GN=bglS PE=1 SV=2
sp|P07980|GUB_BACAM	3.18e-11	138	264	98	217	Beta-glucanase OS=Bacillus amyloliquefaciens OX=1390 GN=bglA PE=3 SV=1
sp|P27051|GUB_BACLI	1.12e-10	138	264	102	221	Beta-glucanase OS=Bacillus licheniformis OX=1402 GN=bg1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000227	0.999748	CS pos: 24-25. Pr: 0.9718

TMHMM  Annotations     

There is no transmembrane helices in OAG23253.1.