CAZyme Information: NCU03209-t26_1-p1

Basic Information

• Species: Neurospora crassa
• Lineage: Ascomycota; Sordariomycetes; ; Sordariaceae; Neurospora; Neurospora crassa
• CAZyme ID: NCU03209-t26_1-p1
• CAZy Family: CE4
• CAZyme Description: chitinase 1

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
426	CM002236|CGC30	47082.20	7.7687

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_NcrassaOR74A	10591	367110	833	9758

• Gene Location: location=CM002236:4911213-4912670(+)

Full Sequence      

MLSSIVTCVIPLLVISTPAAAATSQFQVQSSGNTTVSSYRNAVYFTNWGIYGANFQPQQL
PASQITHVLYSFADIKPDGQVVSSDTYADLEKRYPGDSWSEPGENAYGCVKQMYLLKKRN
RNMKVLLSIGGWTYSPKFPPVAATEEGRRRFASSAVKLVQDWGFDGLDIDWEYPTNAREA
QDFVLLLRACRQALDDYARQYAPGYHFLITIAAPAGPQHYGVMDLPGMNPYIDSWHLMAY
DYAGSWDSTTGHQANLLPSPKNLLTTRFNTDQAVRDFVRRGIPANKIVLGLPLYGRSFEG
TDGLGKPYSGIGAGTLEPGTWVYRDLPRPGAKEEYDNLAKATYSYDALSRELITYDNVLS
ALVKTKYIFLRGLGGAVFWEASGDKTGAESLIGTLATQMKRLDQTKNLLAYPASRYANIR
AGVPES

Enzyme Prediction     

EC	3.2.1.14:58

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH18	38	389	3.4e-78	0.956081081081081

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
119365	GH18_chitinase	4.40e-129	41	384	1	322	The GH18 (glycosyl hydrolases, family 18) type II chitinases hydrolyze chitin, an abundant polymer of N-acetylglucosamine and have been identified in bacteria, fungi, insects, plants, viruses, and protozoan parasites. The structure of this domain is an eight-stranded alpha/beta barrel with a pronounced active-site cleft at the C-terminal end of the beta-barrel.
214753	Glyco_18	2.44e-122	40	384	1	334	Glyco_18 domain.
395573	Glyco_hydro_18	2.87e-94	40	384	1	307	Glycosyl hydrolases family 18.
225862	ChiA	3.75e-88	3	396	8	433	Chitinase, GH18 family [Carbohydrate transport and metabolism].
119351	GH18_chitolectin_chitotriosidase	4.40e-85	44	379	4	336	This conserved domain family includes a large number of catalytically inactive chitinase-like lectins (chitolectins) including YKL-39, YKL-40 (HCGP39), YM1, oviductin, and AMCase (acidic mammalian chitinase), as well as catalytically active chitotriosidases. The conserved domain is an eight-stranded alpha/beta barrel fold belonging to the family 18 glycosyl hydrolases. The fold has a pronounced active-site cleft at the C-terminal end of the beta-barrel. The chitolectins lack a key active site glutamate (the proton donor required for hydrolytic activity) but retain highly conserved residues involved in oligosaccharide binding. Chitotriosidase is a chitinolytic enzyme expressed in maturing macrophages, which suggests that it plays a part in antimicrobial defense. Chitotriosidase hydrolyzes chitotriose, as well as colloidal chitin to yield chitobiose and is therefore considered an exochitinase. Chitotriosidase occurs in two major forms, the large form being converted to the small form by either RNA or post-translational processing. Although the small form, containing the chitinase domain alone, is sufficient for the chitinolytic activity, the additional C-terminal chitin-binding domain of the large form plays a role in processing colloidal chitin. The chitotriosidase gene is nonessential in humans, as about 35% of the population are heterozygous and 6% homozygous for an inactivated form of the gene. HCGP39 is a 39-kDa human cartilage glycoprotein thought to play a role in connective tissue remodeling and defense against pathogens.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
EAA36073.3|GH18	0.0	1	426	1	426
AEO70479.1|GH18	2.34e-199	30	424	91	488
VBB72338.1|GH18	8.36e-188	28	424	101	501
QBZ56002.1|GH18	1.14e-187	14	415	95	496
CDP23242.1|GH18	4.37e-174	28	424	101	485

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1D2K_A	1.09e-162	39	424	3	391	Chain A, CHITINASE 1 [Coccidioides immitis],1LL4_A Chain A, Chitinase 1 [Coccidioides immitis],1LL4_B Chain B, Chitinase 1 [Coccidioides immitis],1LL4_C Chain C, Chitinase 1 [Coccidioides immitis],1LL4_D Chain D, Chitinase 1 [Coccidioides immitis]
1LL7_A	3.12e-162	39	424	3	391	Chain A, CHITINASE 1 [Coccidioides immitis],1LL7_B Chain B, CHITINASE 1 [Coccidioides immitis]
1LL6_A	6.27e-162	39	424	3	391	Chain A, CHITINASE 1 [Coccidioides immitis],1LL6_B Chain B, CHITINASE 1 [Coccidioides immitis],1LL6_C Chain C, CHITINASE 1 [Coccidioides immitis],1LL6_D Chain D, CHITINASE 1 [Coccidioides immitis]
6EPB_A	5.25e-161	37	423	36	422	Structure of Chitinase 42 from Trichoderma harzianum [Trichoderma harzianum]
6YLJ_A	6.97e-159	37	423	36	422	Chain A, Endochitinase 42 [Trichoderma harzianum],6YN4_A Chain A, Endochitinase 42 [Trichoderma harzianum],7AKQ_A Chain A, Endochitinase 42 [Trichoderma harzianum]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P32470|CHI1_APHAL	5.03e-163	37	423	36	422	Chitinase 1 OS=Aphanocladium album OX=12942 GN=CHI1 PE=1 SV=2
sp|Q1E3R8|CHI1_COCIM	1.90e-161	39	424	38	426	Endochitinase 1 OS=Coccidioides immitis (strain RS) OX=246410 GN=CTS1 PE=3 SV=1
sp|P0CB51|CHI1_COCPS	1.90e-161	39	424	38	426	Endochitinase 1 OS=Coccidioides posadasii (strain RMSCC 757 / Silveira) OX=443226 GN=CTS1 PE=1 SV=1
sp|C5P230|CHI1_COCP7	3.82e-161	39	424	38	426	Endochitinase 1 OS=Coccidioides posadasii (strain C735) OX=222929 GN=CTS1 PE=3 SV=1
sp|P48827|CHI42_TRIHA	6.26e-159	37	423	36	422	Endochitinase 42 OS=Trichoderma harzianum OX=5544 GN=chit42 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000424	0.999555	CS pos: 21-22. Pr: 0.9784

TMHMM  Annotations     

There is no transmembrane helices in NCU03209-t26_1-p1.