CAZyme Information: MELLADRAFT_90001-t26_1-p1

Basic Information

• Species: Melampsora larici-populina
• Lineage: Basidiomycota; Pucciniomycetes; ; Melampsoraceae; Melampsora; Melampsora larici-populina
• CAZyme ID: MELLADRAFT_90001-t26_1-p1
• CAZy Family: GT25
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
559		60699.44	8.0713

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_Mlarici-populina98AG31	16380	747676	8	16372

• Gene Location: location=GL883123:507655-510130(-)

Full Sequence      

MKHLLIFKSSSSRTHLGHENIANQHEVHSNIVAGCGIKQLATSNTFFVVLNTQRANTASL
RAKFSKLGIDAVFPGDSSFEKFAAPFNKRLSYIPAAIVFPNHTEAVSNCVKVAVEEKLPV
STRSGGHLYAAYGLGGGNGALVVDVSRLKTIFVHQSTGQAVIGTGNRLGDMAIGLYSQGG
RAIPHGTCPYVGIGGHAAFGGFGTTSRMWGLTLDNIIGHEVVLANGTIVQASQDSNPDLF
WALRGAGASYGIMTSIKFQTHSAPSQATNFHIKWYLSQNELANALLKFQVFCRSNLPSEL
AIDANLKKAPKSGLLVFSLVGTWNGEESKFAAVIQPLLDGMPAPIESLVKRNDWLDSLQT
SARSDNLSTSGVDLSAEHDTFYAKSLTTPQSTPMSNSSIQAFTEYLASEGLKTDTVRNGL
TVYSILNYSFVIICIKQNWFVQFELYGGQNSAITSVAKDATAFAQRSILFTIQFYTSSSN
YSPPFPDAGLTFLDQMVAAIVNNGPSGWEYGAYSNYVDARLSSNEWKNLYYNTHYQRLTQ
TKVAYDPQNIFSYPQSIGE

Enzyme Prediction     

EC	1.1.3.-:1

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
AA7	82	557	2.8e-86	0.9934497816593887

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
396238	FAD_binding_4	1.70e-22	94	232	1	139	FAD binding domain. This family consists of various enzymes that use FAD as a co-factor, most of the enzymes are similar to oxygen oxidoreductase. One of the enzymes Vanillyl-alcohol oxidase (VAO) has a solved structure, the alignment includes the FAD binding site, called the PP-loop, between residues 99-110. The FAD molecule is covalently bound in the known structure, however the residue that links to the FAD is not in the alignment. VAO catalyzes the oxidation of a wide variety of substrates, ranging form aromatic amines to 4-alkylphenols. Other members of this family include D-lactate dehydrogenase, this enzyme catalyzes the conversion of D-lactate to pyruvate using FAD as a co-factor; mitomycin radical oxidase, this enzyme oxidizes the reduced form of mitomycins and is involved in mitomycin resistance. This family includes MurB an UDP-N-acetylenolpyruvoylglucosamine reductase enzyme EC:1.1.1.158. This enzyme is involved in the biosynthesis of peptidoglycan.
223354	GlcD	9.15e-18	92	268	30	211	FAD/FMN-containing dehydrogenase [Energy production and conversion].
178402	PLN02805	7.53e-09	86	322	127	373	D-lactate dehydrogenase [cytochrome]
369658	BBE	1.29e-08	512	558	1	45	Berberine and berberine like. This domain is found in the berberine bridge and berberine bridge- like enzymes which are involved in the biosynthesis of numerous isoquinoline alkaloids. They catalyze the transformation of the N-methyl group of (S)-reticuline into the C-8 berberine bridge carbon of (S)-scoulerine.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QRW15212.1|AA7	3.33e-111	50	558	21	490
QRV86657.1|AA7	3.33e-111	50	558	21	490
QRW00715.1|AA7	7.38e-110	49	558	20	490
CCA69005.1|AA7	5.16e-107	37	557	3	484
QRW27390.1|AA7	5.26e-107	72	558	44	491

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5K8E_A	1.51e-81	83	557	50	492	Xylooligosaccharide oxidase from Myceliophthora thermophila C1 [Thermothelomyces thermophilus ATCC 42464],5L6F_A Xylooligosaccharide oxidase from Myceliophthora thermophila C1 in complex with Xylobiose [Thermothelomyces thermophilus ATCC 42464],5L6G_A Xylooligosaccharide oxidase from Myceliophthora thermophila C1 in complex with Xylose [Thermothelomyces thermophilus ATCC 42464]
3RJ8_A	2.54e-79	59	557	2	469	Crystal structure of carbohydrate oxidase from Microdochium nivale [Microdochium nivale],3RJA_A Crystal structure of carbohydrate oxidase from Microdochium nivale in complex with substrate analogue [Microdochium nivale]
1ZR6_A	2.17e-78	86	557	35	476	The crystal structure of an Acremonium strictum glucooligosaccharide oxidase reveals a novel flavinylation [Sarocladium strictum],2AXR_A Crystal structure of glucooligosaccharide oxidase from Acremonium strictum: a novel flavinylation of 6-S-cysteinyl, 8alpha-N1-histidyl FAD [Sarocladium strictum]
3HSU_A	4.55e-77	86	557	35	476	Chain A, Glucooligosaccharide oxidase [Sarocladium strictum]
6Y0R_AAA	5.44e-75	75	557	42	488	Chain AAA, Chitooligosaccharide oxidase [Fusarium graminearum PH-1]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|E9F5F1|SUBF_METRA	1.96e-81	49	557	18	489	FAD-linked oxidoreductase subF OS=Metarhizium robertsii (strain ARSEF 23 / ATCC MYA-3075) OX=655844 GN=subF PE=3 SV=1
sp|P9WEY2|DPMAF_METAN	2.75e-81	49	557	18	489	FAD-linked oxidoreductase dpmaF OS=Metarhizium anisopliae OX=5530 GN=dpmaF PE=1 SV=1
sp|G2QG48|XYLO_MYCTT	7.78e-81	83	557	50	492	Xylooligosaccharide oxidase OS=Myceliophthora thermophila (strain ATCC 42464 / BCRC 31852 / DSM 1799) OX=573729 GN=xylO PE=1 SV=1
sp|I1SB12|MNCO_MICNN	2.34e-78	59	557	24	491	Carbohydrate oxidase OS=Microdochium nivale OX=5520 GN=MnCO PE=1 SV=2
sp|Q6PW77|GOOX_SARSR	1.01e-77	86	557	54	495	Glucooligosaccharide oxidase OS=Sarocladium strictum OX=5046 GN=gluO PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000018	0.000023

TMHMM  Annotations     

There is no transmembrane helices in MELLADRAFT_90001-t26_1-p1.