CAZyme Information: MELLADRAFT_87218-t26_1-p1

Basic Information

• Species: Melampsora larici-populina
• Lineage: Basidiomycota; Pucciniomycetes; ; Melampsoraceae; Melampsora; Melampsora larici-populina
• CAZyme ID: MELLADRAFT_87218-t26_1-p1
• CAZy Family: GT15
• CAZyme Description: family 28 glycoside hydrolase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
407		45154.01	10.1383

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_Mlarici-populina98AG31	16380	747676	8	16372

• Gene Location: location=GL883090:3902152-3904558(-)

Full Sequence      

MDFGAVANNRTDIGPALMKAFECAQKSITKRAADTVILVPPGNYRWASKVVFAKSQFITV
TIRGRLYMPYDPKLTRHLNVPPMNSRQIIRSSNIFLKIIFNGNGRLYGNGNNYRPGRNLN
LHPNRPRLIRFERCDTVDYSGVDLYDAPLFHLVFSHGKNIRIHHFEIHCLYIGATDGINV
SGSNIYVHDVVVENGDELTDKANFDRRCVTAKSPIIGLKVRNVQCIGTTGCAIGSMGPTA
KDYRIENLDYRNVTVSNGANGVVIKSYPGASGIISLYPLPNQKRDVLKLQIVTDKCALDI
LSSFTLIAQMKTNVAYPIKLDYMWGATLHKTRRQKPVEEIQRWSGMTFNDFSGTGTKNRA
PVTLNCPGRSPCTGLQLTGSTKTTVVSNACGRVDSKSRKTIPNLPPC

Enzyme Prediction     

EC	3.2.1.171:11

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH28	25	380	1.6e-30	0.9138461538461539

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
395231	Glyco_hydro_28	4.97e-19	100	389	63	315	Glycosyl hydrolases family 28. Glycosyl hydrolase family 28 includes polygalacturonase EC:3.2.1.15 as well as rhamnogalacturonase A(RGase A), EC:3.2.1.-. These enzymes are important in cell wall metabolism.
178580	PLN03003	2.12e-17	3	393	29	367	Probable polygalacturonase At3g15720
215426	PLN02793	1.02e-13	2	407	57	422	Probable polygalacturonase
177865	PLN02218	3.05e-09	2	377	72	403	polygalacturonase ADPG
215540	PLN03010	4.56e-09	1	372	50	364	polygalacturonase

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CAE7186140.1|GH28	2.55e-32	1	377	42	352
ULM60707.1|GH28	1.04e-29	2	391	91	436
AEO70856.1|GH28	4.10e-29	1	375	47	355
ACI26689.1|GH28|3.2.1.171	1.42e-28	11	377	54	354
UPK95385.1|GH28	1.50e-28	1	375	44	355

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1RMG_A	1.93e-22	1	377	24	333	Rhamnogalacturonase A From Aspergillus Aculeatus [Aspergillus aculeatus]
6KVE_A	6.29e-08	106	388	88	337	Chain A, Endo-polygalacturonase [Evansstolkia leycettana]
6KVH_A	2.64e-07	106	388	88	337	Chain A, endo-polygalacturonase [Evansstolkia leycettana]
7E56_A	1.43e-06	106	388	80	329	Chain A, Endo-polygalacturonase [Evansstolkia leycettana]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|A2QWT2|RHGD_ASPNC	1.22e-28	1	378	40	350	Putative rhamnogalacturonase D OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=rhgD PE=3 SV=1
sp|B8NK45|RHGA_ASPFN	2.32e-26	2	377	44	353	Probable rhamnogalacturonase A OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=rhgA PE=3 SV=1
sp|A2QYE5|RHGA_ASPNC	3.52e-26	1	377	42	352	Probable rhamnogalacturonase A OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=rhgA PE=3 SV=1
sp|Q2U293|RHGA_ASPOR	5.86e-26	2	377	44	353	Probable rhamnogalacturonase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=rhgA PE=3 SV=1
sp|B8NKB9|RHGE_ASPFN	1.26e-25	1	400	45	393	Probable rhamnogalacturonase E OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=rhgE PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000048	0.000003

TMHMM  Annotations     

There is no transmembrane helices in MELLADRAFT_87218-t26_1-p1.