CAZyme Information: MELLADRAFT_42252-t26_1-p1

Basic Information

• Species: Melampsora larici-populina
• Lineage: Basidiomycota; Pucciniomycetes; ; Melampsoraceae; Melampsora; Melampsora larici-populina
• CAZyme ID: MELLADRAFT_42252-t26_1-p1
• CAZy Family: GH18
• CAZyme Description: family 2 glycosyltransferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
893	GL883095|CGC5	100693.08	7.4828

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_Mlarici-populina98AG31	16380	747676	8	16372

• Gene Location: location=GL883095:187804-191782(+)

Full Sequence      

MHQVPGGRIGPRSHLNSNASYDSLPARPPYPPTHSSTRTHDYGQQAVYGGSGLHEEEFDS
SQWVPASQHPASHYAPSAHGTDPFQEHHLSFPTARHSEPSHESSHEDIPLMTGDEYPDPY
NAGGFVPAPEPDDSYVRYGRIPQRQPRRYKTVKRVELYHGNLVLDCKVPSKLLAMCPKKD
DREFTHMRYTAATCDPNDFKDERYTLRQVLYEPARRTELFIVMTMYNEDDQLFTRTMHGV
MKNVQHLCSRDRSKTWGKDGWKKVVVCIVSDGRSKINSRTLSVLAAMGVYQDGVAKNVVN
DKPVTAHIYEYTTQISIDPEMKFKGAEKGLVPVQILFCLKEKNQKKINSHRWFFNAFGPV
LQPNVCVLLDVGTQPGVSSIYRLWKSFDVNSNVGGACGEIVALKGKLLRNLLNPLVAAQN
FEYKMSNILDKPLESVFGYITVLPGAFSAYRYIALQNDARGEGPLTQYFKGETLGHSADA
DLWTKNMYLAEDRILCWELVSKRNSSWILHYVKNSYAVTDVPDQVPELISQRRRWLNGSF
FAAIHSTVHFGYLYRSSHSFFRKAILHFEMVYQTFSMLFAWFGLANYYIAFVILTQSMED
PSFGLNGIKYFNDVLRYIYLGILIMCFLLAMGNRPQGSNIMFTTALLVFAFITVYMTFAG
IFISVKGLMNAEAAISAGGQKFDVGQLFANSIFRNIVMSLVATYGLWLVASFMFFEPWHM
FTSFLQYILMSPSYINVINVYAFCNVHDVSWGTKGDNKVETDLGVVKPAEDGKTNKVEIS
VPTDEKDINAAYDDACHVLSVKPPVEEEHIDAETKMTDSYRNIRTNVVLAWAIMNGALVA
VILSTGASSNIAADGNNAKVNAYMTFLLYSVFGLSFVKFCGATMYLVQFIFTG

Enzyme Prediction     

EC	2.4.1.16:26	2.4.1.16:26

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT2	225	387	3.9e-82	0.9938650306748467

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
396286	Chitin_synth_1	8.79e-122	225	387	1	163	Chitin synthase. This region is found commonly in chitin synthases classes I, II and III. Chitin a linear homopolymer of GlcNAc residues, it is an important component of the cell wall of fungi and is synthesized on the cytoplasmic surface of the cell membrane by membrane bound chitin synthases.
133033	Chitin_synth_C	1.36e-92	221	542	1	244	C-terminal domain of Chitin Synthase catalyzes the incorporation of GlcNAc from substrate UDP-GlcNAc into chitin. Chitin synthase, also called UDP-N-acetyl-D-glucosamine:chitin 4-beta-N-acetylglucosaminyltransferase, catalyzes the incorporation of GlcNAc from substrate UDP-GlcNAc into chitin, which is a linear homopolymer of GlcNAc residues formed by covalent beta-1,4 linkages. Chitin is an important component of the cell wall of fungi and bacteria and it is synthesized on the cytoplasmic surface of the cell membrane by membrane bound chitin synthases. Studies with fungi have revealed that most of them contain more than one chitin synthase gene. At least five subclasses of chitin synthases have been identified.
400626	Chitin_synth_1N	7.94e-37	154	224	1	70	Chitin synthase N-terminal. This is the N-terminal domain of Chitin synthase (pfam01644).
224136	BcsA	3.69e-28	336	754	115	422	Glycosyltransferase, catalytic subunit of cellulose synthase and poly-beta-1,6-N-acetylglucosamine synthase [Cell motility].
133045	CESA_like	1.36e-14	221	455	1	166	CESA_like is the cellulose synthase superfamily. The cellulose synthase (CESA) superfamily includes a wide variety of glycosyltransferase family 2 enzymes that share the common characteristic of catalyzing the elongation of polysaccharide chains. The members include cellulose synthase catalytic subunit, chitin synthase, glucan biosynthesis protein and other families of CESA-like proteins. Cellulose synthase catalyzes the polymerization reaction of cellulose, an aggregate of unbranched polymers of beta-1,4-linked glucose residues in plants, most algae, some bacteria and fungi, and even some animals. In bacteria, algae and lower eukaryotes, there is a second unrelated type of cellulose synthase (Type II), which produces acylated cellulose, a derivative of cellulose. Chitin synthase catalyzes the incorporation of GlcNAc from substrate UDP-GlcNAc into chitin, which is a linear homopolymer of beta-(1,4)-linked GlcNAc residues and Glucan Biosynthesis protein catalyzes the elongation of beta-1,2 polyglucose chains of Glucan.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ACT75572.1|GT2	0.0	6	893	12	908
ABB70407.1|GT2	0.0	2	893	4	894
CDR44618.1|GT2	0.0	113	891	217	992
VWO98184.1|GT2	0.0	107	893	142	930
QYK92505.1|GT2	0.0	132	893	208	969

PDB Hits     

MELLADRAFT_42252-t26_1-p1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P87073|CHS1_PHYB8	0.0	117	893	59	841	Chitin synthase 1 OS=Phycomyces blakesleeanus (strain ATCC 8743b / DSM 1359 / FGSC 10004 / NBRC 33097 / NRRL 1555) OX=763407 GN=chs1 PE=3 SV=1
sp|J9VGT6|CHS8_CRYNH	0.0	14	893	177	1031	Chitin synthase 8 OS=Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) OX=235443 GN=CHS8 PE=3 SV=2
sp|P30595|CHS2_RHIOL	0.0	136	893	104	858	Chitin synthase 2 OS=Rhizopus oligosporus OX=4847 GN=CHS2 PE=3 SV=1
sp|Q99127|CHS4_USTMA	0.0	34	893	93	941	Chitin synthase 4 OS=Ustilago maydis (strain 521 / FGSC 9021) OX=237631 GN=CHS4 PE=3 SV=2
sp|P30594|CHS1_RHIOL	2.06e-307	139	893	105	858	Chitin synthase 1 OS=Rhizopus oligosporus OX=4847 GN=CHS1 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000053	0.000000

TMHMM  Annotations     

Start	End
571	593
614	631
641	663
692	714
724	746
825	847
867	889