CAZyme Information: MELLADRAFT_22334-t26_1-p1

Basic Information

• Species: Melampsora larici-populina
• Lineage: Basidiomycota; Pucciniomycetes; ; Melampsoraceae; Melampsora; Melampsora larici-populina
• CAZyme ID: MELLADRAFT_22334-t26_1-p1
• CAZy Family: CE5
• CAZyme Description: family 24 glycosyltransferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1483	GL883101|CGC2	166363.66	5.5599

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_Mlarici-populina98AG31	16380	747676	8	16372

• Gene Location: location=GL883101:770848-777089(-)

Full Sequence      

SPPISVSIQATWPEPPLLIQIIESAAIERSDSFFPILTAVSKHWRTIDSLSLTPQQIYNQ
TLNQITLNGLLNDQGEIESLETSIALKEASPKIEAFRAWYRDRPAPRILSFDHICPPGSH
IPPPSVPVVILYASNDPRSFGPFHDVLHQASSSSPIRATYIFRWQVSSDDSQSSSIGPSH
LSGWATRLDIKKSDYLTVDDRPVETNSRESRDSESESTKENTKEDAPETSTVIIPLQADE
FSTIGLKATQHILTSPNPLSALRVLSEDFPRHAYNLVKNWVPGEINADLDRELKENAAEG
VVKPGQSAIWINGLDISSVLPVANINFFKLIQLMRDERRWIKSLTSLGLSVSEIRKLVTD
EQLNSALSGEGGARSDDLEIDPSILGERFDASDRLEHGEMILWLNDLEKDTRYAAWPKSL
RDFLRPTYPGQLHAVARNTITVVLALNVTHTANLRILTESVETFITRSVPIRWGLVPLDD
PNASGSSEALKGIFPIYSSGTSTGSTSSDMVDALDFKQRADAYRAAHPESDSDDQKDTAS
FGEGDFNSWSEKTQSYIRRLALCSGSGEVPGCMFVNGKFFVLDDDFRMNLQQTAMLHIQF
LQHQIYYGLIQGSVDVSHYLYDLPGVYPARNEFVFPSPGRPVRVVNLVQAFVAAKLGSCA
VEADHETNEPPISAIWVIGNLDSKSGAASVLALLELMVNTDMCFPLLEQCGDMLHLTFTL
QAFPSDQTRGSVFVGFVHASSQQTDAHIDPRLSDSLGQLIHSPEHPGILIKNVTDQFAHF
GLQPSKPFPESFSLSKMPSEWRRSTEDGEDVWAGAQSLITELDITEGHVAVVINGRVLDV
PHSQVLQASDLRMLIDYEIQKRTNPLQVALRSVLGPERFTTIKSMVPYIASVISSVLVSS
DDEAGSKTRGRAVSNALTERKHSSISYGDHDAAVIQFRVILNPASELAQSWSALLETLSG
RKDVAIKVWFNPMQHVEELPIKRFLRTSFHSRIQFDQDGSSVSPSVVFKHMPTDVLLTLS
IEAPPAWLALPLESVHDLDNIMLSKIGSTERERGVEGVFQLEHIIIAGHASELPSEVPPR
GLQVVLTNLLTSASVDTIIMANLGYFQFKAEPGMFVMKIRPGRSSELYTLERVDLKTGKD
RLSTDKNKTDEDHQFSLTTFDGLLLFPRFRKRPGKEKEKLIQPVAAMSESEDAHTSTTQK
LIGHKPSDVIPGKKRNVINVFTVASGLLYERMAYLMCVSVMRHTKSDVKFWFISNFLSPS
FKRFIPHLARKYEFEYELVTYRWPPWLRAQKEKQRVIWGYKILFLDVLFPLELDRVIFVD
SDQIVRTDLQELVDLDLRGAPYAYTPMCNDREETKGFRFWDTGYWKDSLRGRPYHISALY
VVDLRVFRAVAAGDQLRQHYQALSADPNSLANLDQDLPNNMQEVLPIYSLDQKWLWCETW
CSDDGLKTAKTIDLCNNPLTHEPKLTRARRLIPEWDVYDKEVA

Enzyme Prediction     

EC	2.4.1.-:29

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT24	1218	1465	1.3e-125	0.9959677419354839

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
408203	Glyco_transf_24	0.0	1218	1483	1	266	Glucosyltransferase 24. This is the catalytic domain found in UDP-glucose:glycoprotein glucosyltransferase (UGGT). This domain belongs to glucosyltransferase 24 family (GT24) A-type domain. The GT domain displays the expected glycosyltransferase type A (GT-A) fold.
133054	GT8_HUGT1_C_like	2.97e-166	1218	1465	1	248	The C-terminal domain of HUGT1-like is highly homologous to the GT 8 family. C-terminal domain of glycoprotein glucosyltransferase (UGT). UGT is a large glycoprotein whose C-terminus contains the catalytic activity. This catalytic C-terminal domain is highly homologous to Glycosyltransferase Family 8 (GT 8) and contains the DXD motif that coordinates donor sugar binding, characteristic for Family 8 glycosyltransferases. GT 8 proteins are retaining enzymes based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. The non-catalytic N-terminal portion of the human UTG1 (HUGT1) has been shown to monitor the protein folding status and activate its glucosyltransferase activity.
408201	Thioredoxin_14	2.23e-59	389	634	1	248	Thioredoxin-like domain. This is the third out of four TRXL(thioredoxin-like) domains found in UDP-glucose:glycoprotein glucosyltransferase (UGGT).
132996	Glyco_transf_8	2.70e-54	1218	1458	1	241	Members of glycosyltransferase family 8 (GT-8) are involved in lipopolysaccharide biosynthesis and glycogen synthesis. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. GT-8 comprises enzymes with a number of known activities: lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, glycogenin glucosyltransferase, and N-acetylglucosaminyltransferase. GT-8 enzymes contains a conserved DXD motif which is essential in the coordination of a catalytic divalent cation, most commonly Mn2+.
399437	UDP-g_GGTase	3.68e-40	1068	1182	2	109	UDP-glucose:Glycoprotein Glucosyltransferase. UDP-g_GGTase is an important, central component of the QC system in the ER for checking that glycoproteins are folded correctly. This QC prevents incorrectly folded glycoproteins from leaving the ER.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CDR47046.1|GT24	1.09e-298	1	1483	23	1628
CCA74280.1|GT24	2.02e-290	3	1483	26	1521
QRV75871.1|GT24	4.23e-287	1	1483	23	1528
QRV90671.1|GT24	3.28e-286	1	1483	23	1528
ADV20593.1|GT24	4.10e-278	1	1483	20	1492

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5MU1_A	1.28e-245	1	1483	8	1445	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5MZO_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TRF_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TRT_A	2.53e-245	1	1483	8	1445	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
5NV4_A	3.79e-244	1	1483	8	1445	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TS8_A	1.03e-211	1	1427	1	1382	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS8_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TS2_A	4.29e-192	813	1483	532	1211	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_C Chain C, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_D Chain D, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q09140|UGGG_SCHPO	3.27e-232	3	1483	21	1422	UDP-glucose:glycoprotein glucosyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=gpt1 PE=1 SV=2
sp|Q9JLA3|UGGG1_RAT	9.66e-191	102	1482	173	1520	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Rattus norvegicus OX=10116 GN=Uggt1 PE=1 SV=2
sp|Q6P5E4|UGGG1_MOUSE	1.34e-190	4	1482	47	1520	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Mus musculus OX=10090 GN=Uggt1 PE=1 SV=4
sp|Q09332|UGGG_DROME	1.76e-190	3	1482	29	1504	UDP-glucose:glycoprotein glucosyltransferase OS=Drosophila melanogaster OX=7227 GN=Uggt PE=1 SV=2
sp|Q9NYU2|UGGG1_HUMAN	2.07e-189	90	1482	161	1520	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Homo sapiens OX=9606 GN=UGGT1 PE=1 SV=3

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000036	0.000001

TMHMM  Annotations     

There is no transmembrane helices in MELLADRAFT_22334-t26_1-p1.