CAZyme Information: MELLADRAFT_114892-t26_1-p1

Basic Information

• Species: Melampsora larici-populina
• Lineage: Basidiomycota; Pucciniomycetes; ; Melampsoraceae; Melampsora; Melampsora larici-populina
• CAZyme ID: MELLADRAFT_114892-t26_1-p1
• CAZy Family: AA7
• CAZyme Description: family 2 glycoside hydrolase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1031		115453.48	6.0158

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_Mlarici-populina98AG31	16380	747676	8	16372

• Gene Location: location=GL883090:2187399-2191832(+)

Full Sequence      

MFQLLPLLALAASAAVQTVLSADTTRGPPLPNAHLDLSDLKWTLSNANKSISVPTSFVNQ
AHLALIDAGIIDDPNIGLNEGTTRWVGEEKAWLWQTNVDLSEIFKWEQVDQYFFAFQGLD
TFCEIKLGPHLIGTTDNAFRSWVFNATDAIHKLGPSTHSTTLSLRFDSPWGVATRLAHEP
GNLWYPQAGLGNPNVADTLVIYEYDARTHNASSYNGWLNRILFTFLGPAYLPSGPLKPGY
LIGLARTATTETRPAAPRDPAHGDSQTVVTIKPPTTGQKDESDFEPYWIHKTVIDIYRQG
QRNNLPPDQNAPWVINITLPLVTPHPPSRFASASLSGTLRGTNIKFAPVRLRALPLPAYS
TDGPQVYLTASFSVAPNAVEKWYPATLGNPKLYELGLSFDSGFSPQTQWDERVGFRTIIV
DQSQYTEQEVARGVTPGSKFNLQINGKPFFVQGSSIIPIDNFAARANSSTITWLLKSAVL
AHQNVIRIWGGGAYETDEFYDLCDEIGVLAWSESVFACGAYPIEPKHILDNVLAETAENV
QRLGRHPSVALWAGNNEPRITFADPKHLIQKGEGYIEWINGTWNNGSVYFKQYDYLYNHA
IRDVLMDNTRSISYMPSSTTDGYLTLDPYVSRYGKHVPGEIDGDKELYNYNTAISFDTKN
YPVARFVNEFGFHSMPSIYTWDRILTNIEAYSFNSSEIRVHTKHPPAKSLTYPFAADDGQ
KEMTTGVTDWYPTPNITGDPRELLAQWAYSTQVFQAAFMASEITYYRLGASRGENNMGAL
YWQLNDVWEGVSWSSIEYTGRWKIFHYVAERVQDHVIISPMFNRTNQTLDVYATSDLWYP
VTGNAKWTWYDFAGKQLASANQTFSIGPINSTHLFRAVGLEDMVPGSTPSNDAWLHLQLT
ASSQTSGQSFYNEQFFHPVPLKDAALRASHVTVTVTGKNRFNIIGHGAVAAWVNVEPPPA
ILRDVFQLGVTGYFMDSRTKKPSNCFFLRPDEVRVLDFVVYEDNTNGEFSSKIHVRTLWD
NYHKSNTPTTT

Enzyme Prediction     

EC	3.2.1.25:4

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	45	907	4.9e-54	0.7007978723404256

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
225789	LacZ	1.30e-31	379	812	250	628	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
407658	Mannosidase_ig	2.25e-11	827	923	1	91	Mannosidase Ig/CBM-like domain. This domain corresponds to domain 4 in the structure of Bacteroides thetaiotaomicron beta-mannosidase, BtMan2A. This domain has an Ig-like fold.
407625	Ig_mannosidase	7.67e-10	928	1020	12	78	Ig-fold domain. This Ig-like fold domain is found in mannosidase enzymes.
236673	ebgA	1.45e-04	369	557	273	449	cryptic beta-D-galactosidase subunit alpha; Reviewed

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BCS20939.1|GH2	1.48e-174	35	1020	23	920
BCS16350.1|GH2	1.92e-169	28	1020	14	927
BCS04781.1|GH2	1.92e-169	28	1020	14	927
CAK96951.1|GH2|3.2.1.25	1.56e-167	36	1020	26	926
A2QWU9|GH2	1.56e-167	36	1020	26	926

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4CVU_A	1.31e-160	9	1020	6	938	Structure of Fungal beta-mannosidase from Glycoside Hydrolase Family 2 of Trichoderma harzianum [Trichoderma harzianum],4UOJ_A Chain A, BETA-MANNOSIDASE GH2 [Trichoderma harzianum],4UOJ_B Chain B, BETA-MANNOSIDASE GH2 [Trichoderma harzianum]
6DDT_A	6.61e-73	42	837	18	699	mouse beta-mannosidase (MANBA) [Mus musculus],6DDU_A mouse beta-mannosidase bound to beta-D-mannose (MANBA) [Mus musculus]
2VJX_A	4.84e-54	37	932	10	765	Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]
2JE8_A	4.93e-54	37	932	12	767	Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JE8_B Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
7OP6_A	4.97e-54	37	932	12	767	Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP6_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_A Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q0CI48|MANBA_ASPTN	2.68e-173	36	1025	27	932	Beta-mannosidase A OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=mndA PE=3 SV=2
sp|A2QWU9|MANBA_ASPNC	2.76e-168	36	1020	26	926	Beta-mannosidase A OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=mndA PE=3 SV=1
sp|Q5BCI8|MANBA_EMENI	4.98e-168	15	1020	7	923	Beta-mannosidase A OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=mndA PE=3 SV=1
sp|B0Y7S2|MANBA_ASPFC	4.67e-159	17	1020	6	921	Beta-mannosidase A OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=mndA PE=3 SV=1
sp|Q4WMS9|MANBA_ASPFU	6.56e-159	17	1020	6	921	Beta-mannosidase A OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=mndA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000218	0.999752	CS pos: 21-22. Pr: 0.9572

TMHMM  Annotations     

There is no transmembrane helices in MELLADRAFT_114892-t26_1-p1.