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CAZyme Information: KZZ97822.1

You are here: Home > Sequence: KZZ97822.1

Basic Information | Genomic context | Full Sequence | Enzyme annotations |  CAZy signature domains |  CDD domains | CAZyme hits | PDB hits | Swiss-Prot hits | SignalP and Lipop annotations | TMHMM annotations

Basic Information help

Species Ascosphaera apis
Lineage Ascomycota; Eurotiomycetes; ; Ascosphaeraceae; Ascosphaera; Ascosphaera apis
CAZyme ID KZZ97822.1
CAZy Family GT50
CAZyme Description Beta-N-acetylhexosaminidase [Source:UniProtKB/TrEMBL;Acc:A0A162ISH2]
CAZyme Property
Protein Length CGC Molecular Weight Isoelectric Point
2556 285520.87 6.2549
Genome Property
Genome Version/Assembly ID Genes Strain NCBI Taxon ID Non Protein Coding Genes Protein Coding Genes
FungiDB-61_AapisARSEF7405 6442 392613 0 6442
Gene Location

Full Sequence      Download help

Enzyme Prediction      help

EC 3.2.1.52:13

CAZyme Signature Domains help

Family Start End Evalue family coverage
GH20 2185 2500 2.7e-58 0.8160237388724035

CDD Domains      download full data without filtering help

Cdd ID Domain E-Value qStart qEnd sStart sEnd Domain Description
406558 DOCK_N 4.61e-105 188 582 1 377
DOCK N-terminus. This family is found near to the N-terminus of dedicator of cytokinesis (DOCK) proteins, between the variant SH3 domain (pfam07653) and the C2 domain (pfam14429).
212566 DHR2_DOCK 1.67e-98 1421 1816 1 392
Dock Homology Region 2, a GEF domain, of Dedicator of Cytokinesis proteins. DOCK proteins comprise a family of atypical guanine nucleotide exchange factors (GEFs) that lack the conventional Dbl homology (DH) domain. As GEFs, they activate the small GTPases Rac and Cdc42 by exchanging bound GDP for free GTP. They are also called the CZH (CED-5, Dock180, and MBC-zizimin homology) family, after the first family members identified. Dock180 was first isolated as a binding partner for the adaptor protein Crk. The Caenorhabditis elegans protein, Ced-5, is essential for cell migration and phagocytosis, while the Drosophila ortholog, Myoblast city (MBC), is necessary for myoblast fusion and dorsal closure. DOCKs are divided into four classes (A-D) based on sequence similarity and domain architecture: class A includes Dock1 (or Dock180), 2 and 5; class B includes Dock3 and 4; class C includes Dock6, 7, and 8; and class D includes Dock9, 10 and 11. All DOCKs contain two homology domains: the DHR-1 (Dock homology region-1), also called CZH1, and DHR-2 (also called CZH2 or Docker). This alignment model represents the DHR-2 domain of DOCK proteins, which contains the catalytic GEF activity for Rac and/or Cdc42.
119332 GH20_HexA_HexB-like 4.89e-95 2184 2528 48 348
Beta-N-acetylhexosaminidases catalyze the removal of beta-1,4-linked N-acetyl-D-hexosamine residues from the non-reducing ends of N-acetyl-beta-D-hexosaminides including N-acetylglucosides and N-acetylgalactosides. The hexA and hexB genes encode the alpha- and beta-subunits of the two major beta-N-acetylhexosaminidase isoenzymes, N-acetyl-beta-D-hexosaminidase A (HexA) and beta-N-acetylhexosaminidase B (HexB). Both the alpha and the beta catalytic subunits have a TIM-barrel fold and belong to the glycosyl hydrolase family 20 (GH20). The HexA enzyme is a heterodimer containing one alpha and one beta subunit while the HexB enzyme is a homodimer containing two beta-subunits. Hexosaminidase mutations cause an inability to properly hydrolyze certain sphingolipids which accumulate in lysosomes within the brain, resulting in the lipid storage disorders Tay-Sachs and Sandhoff. Mutations in the alpha subunit cause in a deficiency in the HexA enzyme and result in Tay-Sachs, mutations in the beta-subunit cause in a deficiency in both HexA and HexB enzymes and result in Sandhoff disease. In both disorders GM(2) gangliosides accumulate in lysosomes. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by solvent or the enzyme, but by the substrate itself.
395590 Glyco_hydro_20 3.49e-79 2185 2500 49 345
Glycosyl hydrolase family 20, catalytic domain. This domain has a TIM barrel fold.
212569 DHR2_DOCK_B 1.50e-50 1421 1814 1 386
Dock Homology Region 2, a GEF domain, of Class B Dedicator of Cytokinesis proteins. DOCK proteins are atypical guanine nucleotide exchange factors (GEFs) that lack the conventional Dbl homology (DH) domain. As GEFs, they activate small GTPases by exchanging bound GDP for free GTP. They are divided into four classes (A-D) based on sequence similarity and domain architecture; class B includes Dock3 and 4. Dock3 is a specific GEF for Rac and it regulates N-cadherin dependent cell-cell adhesion, cell polarity, and neuronal morphology. It promotes axonal growth by stimulating actin polymerization and microtubule assembly. Dock4 activates the Ras family GTPase Rap1, probably indirectly through interaction with Rap regulatory proteins. It plays a role in regulating dendritic growth and branching in hippocampal neurons, where it is highly expressed. All DOCKs contain two homology domains: the DHR-1 (Dock homology region-1), also called CZH1 (CED-5, Dock180, and MBC-zizimin homology 1), and DHR-2 (also called CZH2 or Docker). The DHR-1 domain binds phosphatidylinositol-3,4,5-triphosphate. This alignment model represents the DHR-2 domain of class B DOCKs, which contains the catalytic GEF activity for Rac and/or Cdc42. Class B DOCKs also contain an SH3 domain at the N-terminal region and a PxxP motif at the C-terminus.

CAZyme Hits      help

Hit ID E-Value Query Start Query End Hit Start Hit End
1.10e-173 2078 2555 15 599
1.10e-173 2078 2555 15 599
1.10e-173 2078 2555 15 599
3.89e-173 2078 2555 15 599
3.89e-173 2078 2555 15 599

PDB Hits      download full data without filtering help

Hit ID E-Value Query Start Query End Hit Start Hit End Description
2.59e-159 2188 2555 132 498
Crystal structure of native beta-N-acetylhexosaminidase isolated from Aspergillus oryzae [Aspergillus oryzae],5OAR_D Crystal structure of native beta-N-acetylhexosaminidase isolated from Aspergillus oryzae [Aspergillus oryzae]
1.32e-93 203 1730 92 1562
Chain A, Dedicator of cytokinesis protein 5 [Homo sapiens],7DPA_D Chain D, Dedicator of cytokinesis protein 5 [Homo sapiens]
1.27e-54 1145 1730 964 1536
CryoEM structure of the binary DOCK2-ELMO1 complex [Homo sapiens],6TGB_D CryoEM structure of the binary DOCK2-ELMO1 complex [Homo sapiens],6TGC_A CryoEM structure of the ternary DOCK2-ELMO1-RAC1 complex [Homo sapiens],6TGC_D CryoEM structure of the ternary DOCK2-ELMO1-RAC1 complex [Homo sapiens]
1.05e-39 1407 1730 13 345
Crystal structure of the DHR-2 domain of DOCK2 in complex with Rac1 (T17N mutant) [Homo sapiens],3B13_C Crystal structure of the DHR-2 domain of DOCK2 in complex with Rac1 (T17N mutant) [Homo sapiens]
1.16e-39 1407 1730 18 350
STRUCTURE OF THE COMPLEX BETWEEN Dock2 AND Rac1. [Homo sapiens],2YIN_B STRUCTURE OF THE COMPLEX BETWEEN Dock2 AND Rac1. [Homo sapiens]

Swiss-Prot Hits      download full data without filtering help

Hit ID E-Value Query Start Query End Hit Start Hit End Description
6.91e-174 2078 2555 15 599
Beta-hexosaminidase OS=Aspergillus oryzae OX=5062 GN=nagA PE=1 SV=1
1.16e-159 2074 2555 12 602
Beta-hexosaminidase OS=Emericella nidulans OX=162425 GN=nagA PE=1 SV=1
2.73e-146 2059 2555 1 594
Beta-hexosaminidase 1 OS=Coccidioides posadasii (strain RMSCC 757 / Silveira) OX=443226 GN=HEX1 PE=1 SV=1
5.71e-140 2054 2548 2 607
Probable beta-hexosaminidase ARB_01353 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_01353 PE=1 SV=1
7.00e-112 2185 2554 214 561
Beta-hexosaminidase OS=Candida albicans OX=5476 GN=HEX1 PE=1 SV=1

SignalP and Lipop Annotations help

This protein is predicted as OTHER

Other SP_Sec_SPI CS Position
0.603702 0.396305

TMHMM  Annotations      help

There is no transmembrane helices in KZZ97822.1.