CAZyme Information: KSA01053.1

Basic Information

• Species: Debaryomyces fabryi
• Lineage: Ascomycota; Saccharomycetes; ; Debaryomycetaceae; Debaryomyces; Debaryomyces fabryi
• CAZyme ID: KSA01053.1
• CAZy Family: GH5
• CAZyme Description: Alpha,alpha-trehalase [Source:UniProtKB/TrEMBL;Acc:A0A0V1PXS9]

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1101	LMYN01000065|CGC2	122563.99	4.6890

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_DfabryiCBS789	6025	N/A	0	6025

• Gene Location: location=LMYN01000065:16306-19611(-)

Full Sequence      

MEYDSLGERFKHRAISKVDHCNHFIHKFCKRQIVIKTMIVLNFILLFVLYLYHQGITYAL
PFAIKDVNIDDSFNDSTVTFETIESNRKQLQKLINSRENKEIFLQLQNSGSAYYDPTTNT
VGTAEFPTYNQYQRQAYVSNGYIGSRIPNLGQGFTFDQLSDSPDAVEDDLSNGWPLFNER
FSGSFIGGFYDIQKNTTETNFPELIKKGYESILSAVPQWTTLTLSTVKNGKTFSLDPSLS
RDAQGDISNYAQNLSLSNGIVTTEFTWLDSLQVHIEVVAHRSNINLGIVNLRIVNLGNST
VDLKVEDKLEFASTQRCQLTEVGHDDEGIFIHFQPNEIDYVNGAIYSTLKYDEKSLNSIS
RDSKNDTSTQELDISVEPSKSFKISKMVGIVSSDLDPEKYKTSSSVNDFAKQVATKQADS
VSDLIKSHKTEWDRTFESSNSITFPGDSLLTLASRASIYHLNANTRPGARGVTAALPVGG
LSSDSYGGMVFWDTDLWMLNGLLPFNPDHAKSIVNYRIHTHEQAKKNVPDNKGGAVYSWT
SGRFGNCTSTGPCMDYEYHINIAVAMAAWEVYLSGAADDDYLDSVAYPLINDAATFLANY
VEYNDSLAQYVTHNMTDPDEYANHVDNAAYTNAGISLLMKWAITISNHLGKSVPTKYTDI
AGSMHIPTSDNADNITLEYSGMNSSVGIKQADVIMMTYPLGNELISDDQAYTNMEFYSMK
QVSYGPAMTFPIFSIVASNLSPSGCASQSYLHKAVQPFLRGPFAQFSEQNNDNFLTNGGT
HPAFPFMTAHGGFLQAILQGLTGLRFDYNIDDTNKLSRMLTLDPISLPCLGNGVQFDSIK
YMNKTLSLAINETSFIVKNNGPITGSKDDSPIKISLAKRNPKAGVYTLKKGEELVFPLFE
PEAGSPLSVSECASAKFINITESAYGDATVLVNDGDNTTHWQLKYNDTTAKLLVDLKQPR
NITSGVINWGDRPPKSWSLLAYGSHEQSKIKDLNDNIDFLSKVNFGNNLYKKYEFMDVDM
VYDQDDVFTTIISESVDISAPYDPKESAQIEIPLRHNTTSFAVEQGLNARFLLIEVTDIH
DTEPIDDETGGAKLYEVEFFE

Enzyme Prediction     

EC	3.2.1.28:7

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH65	455	807	6.7e-92	0.9919354838709677

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
224471	ATH1	4.50e-91	138	900	32	772	Trehalose and maltose hydrolase (possible phosphorylase) [Carbohydrate transport and metabolism].
281612	Glyco_hydro_65m	1.59e-40	455	807	1	383	Glycosyl hydrolase family 65 central catalytic domain. This family of glycosyl hydrolases contains vacuolar acid trehalase and maltose phosphorylase.Maltose phosphorylase (MP) is a dimeric enzyme that catalyzes the conversion of maltose and inorganic phosphate into beta-D-glucose-1-phosphate and glucose. The central domain is the catalytic domain, which binds a phosphate ion that is proximal the the highly conserved Glu. The arrangement of the phosphate and the glutamate is thought to cause nucleophilic attack on the anomeric carbon atom. The catalytic domain also forms the majority of the dimerization interface.
397616	Glyco_hydro_65N	3.85e-22	130	393	11	240	Glycosyl hydrolase family 65, N-terminal domain. This family of glycosyl hydrolases contains vacuolar acid trehalase and maltose phosphorylase.Maltose phosphorylase (MP) is a dimeric enzyme that catalyzes the conversion of maltose and inorganic phosphate into beta-D-glucose-1-phosphate and glucose. This domain is believed to be essential for catalytic activity although its precise function remains unknown.
237517	PRK13807	2.75e-20	243	894	102	754	maltose phosphorylase; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CAG87277.2|GH65	0.0	1	1101	1	1100
CAX44942.1|GH65	0.0	25	1100	25	1078
AAV05390.1|GH65|3.2.1.28	0.0	25	1100	48	1101
AOW26348.1|GH65	0.0	25	1100	25	1078
CCE43253.1|GH65|3.2.1.28	0.0	34	1100	30	1071

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7FE3_A	1.67e-48	203	807	67	643	Chain A, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE3_B Chain B, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE3_C Chain C, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE4_A Chain A, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE4_B Chain B, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE4_C Chain C, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101]
3WIQ_A	9.16e-25	369	807	227	696	Crystal structure of kojibiose phosphorylase complexed with kojibiose [Caldicellulosiruptor saccharolyticus DSM 8903],3WIR_A Crystal structure of kojibiose phosphorylase complexed with glucose [Caldicellulosiruptor saccharolyticus DSM 8903],3WIR_B Crystal structure of kojibiose phosphorylase complexed with glucose [Caldicellulosiruptor saccharolyticus DSM 8903],3WIR_C Crystal structure of kojibiose phosphorylase complexed with glucose [Caldicellulosiruptor saccharolyticus DSM 8903],3WIR_D Crystal structure of kojibiose phosphorylase complexed with glucose [Caldicellulosiruptor saccharolyticus DSM 8903]
4KTP_A	2.96e-19	427	876	272	745	Chain A, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTP_B Chain B, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10]
4KTR_A	6.75e-19	427	876	272	745	Chain A, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTR_B Chain B, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTR_C Chain C, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTR_D Chain D, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTR_E Chain E, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTR_F Chain F, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTR_G Chain G, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTR_H Chain H, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q5AAU5|ATC1_CANAL	0.0	25	1100	25	1078	Cell wall acid trehalase ATC1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=ATC1 PE=1 SV=2
sp|P48016|ATH1_YEAST	2.92e-230	100	1008	112	1060	Periplasmic acid trehalase ATH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ATH1 PE=1 SV=1
sp|N1P212|ATH1_YEASC	2.92e-230	100	1008	112	1060	Periplasmic acid trehalase ATC1 OS=Saccharomyces cerevisiae (strain CEN.PK113-7D) OX=889517 GN=CENPK1137D_1727 PE=1 SV=1
sp|M1RNJ8|ATH1_CANGB	5.96e-230	30	1097	79	1208	Periplasmic/secreted acid trehalase ATH1 OS=Candida glabrata OX=5478 GN=ATH1 PE=1 SV=1
sp|D4B5H9|ATC1_ARTBC	1.30e-117	132	982	79	947	Cell wall acid trehalase ARB_03719 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_03719 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000000	0.000019

TMHMM  Annotations     

Start	End
33	55