CAZyme Information: KNZ61702.1

Basic Information

• Species: Puccinia sorghi
• Lineage: Basidiomycota; Pucciniomycetes; ; Pucciniaceae; Puccinia; Puccinia sorghi
• CAZyme ID: KNZ61702.1
• CAZy Family: GT21
• CAZyme Description: uncharacterized protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
710		80369.30	7.3926

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_PsorghiRO10H11247	21769	N/A	691	21078

• Gene Location: location=LAVV01004109:15204-18113(-)

Full Sequence      

MSSASQPEPPTSPRPIRSSVSFDGRHFVDTDSKQIISFRGVSLSGFSKLPSKPDGRTHQA
ELFFEHRDVSFVGHPLRLEHASHYLSQLVNWGFNLIRLVICWEALEHSGPGIYDLEYIQY
IEQLVQLCQQHGLKVLIDAHQDVWSRFCGGSGAPGWTLELAGFEITNLSETAAAALQQLG
APKGVWPSGYQKLAAFFVHSGTMFTLFFAGDAFAPNRKVKRSLHRQWEKEDGEEILISLQ
QFLQGSMIEAFGQLADSLSRFHCVIGFESLANSKCYQPMNEPHRGFINLYSPYQWNPMTD
LFIRDCPSFLQAAALGDGHSQRIDVYTPIWPIPSFRFHTRRIKPQVRAWQRSVECIWKEH
GVWRWDAKRQQPTILNPRHFNIDPISGKPFDFYSQALYPFVSRFAKRIQAHRSDWIIPMG
PIPNEFYPKWDHDQRPQNLVAAPHFYDLFSLVHKSHGTLTMDVQGICMKKPIWKWMHFGH
AAAKRNYAEQIGNIVKSVYKNLGEIPCLIGELGICMDLNKGEAFSTGNFYWQHHQMNALL
GACESNLVSYVLWNFNPYNTDEYGDGWNGENFSFISQSENGGGSSAHSQPRILSAIIRPS
AQKVSGIPCGLSYDPEESTFEFEYKNPDSPSHFTTTATEIFLPTLRFPPDQIHISLSDGG
RHKIKVTSSVRVTIDQKPSPSCGGLFSPPTLLLLTSLLVGLLAFWLISTT

Enzyme Prediction     

EC	3.2.1.45:1

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH5	34	560	1.2e-157	0.9888682745825603

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
408348	Glyco_hydro_5_C	1.97e-13	598	659	1	59	Glycoside hydrolase family 5 C-terminal domain. This is the C-terminal domain of endo-glycoceramidase II (EGC), a membrane-associated family 5 glycosidase pfam00150. The C-terminal domain assumes a beta-sandwich fold, which resembles that of many carbohydrate-binding modules.
395098	Cellulase	2.19e-09	84	142	29	88	Cellulase (glycosyl hydrolase family 5).
225344	BglC	2.73e-08	66	171	52	165	Aryl-phospho-beta-D-glucosidase BglC, GH1 family [Carbohydrate transport and metabolism].
178400	PLN02803	0.004	102	159	130	187	beta-amylase

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QGI82100.1|GH5_12	2.53e-165	20	654	18	697
QGI64843.1|GH5_12	2.53e-165	20	654	18	697
QGI95728.1|GH5_12	2.53e-165	20	654	18	697
CCT68897.1|GH5_12	2.53e-165	20	654	18	697
QKD54802.1|GH5_12	1.00e-164	26	654	24	697

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7LPO_A	4.38e-71	10	576	15	594	Chain A, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99],7LPO_B Chain B, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99],7LPO_C Chain C, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99],7LPO_D Chain D, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99],7LPP_A Chain A, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99],7LPP_B Chain B, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99],7LPP_C Chain C, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99],7LPP_D Chain D, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99],7LPQ_A Chain A, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99],7LPQ_B Chain B, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99],7LPQ_C Chain C, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99],7LPQ_D Chain D, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99]
2OSW_A	2.36e-08	18	165	28	163	Endo-glycoceramidase II from Rhodococcus sp. [Rhodococcus sp.],2OSW_B Endo-glycoceramidase II from Rhodococcus sp. [Rhodococcus sp.],2OYK_A Endo-glycoceramidase II from Rhodococcus sp.: cellobiose-like isofagomine complex [Rhodococcus sp.],2OYK_B Endo-glycoceramidase II from Rhodococcus sp.: cellobiose-like isofagomine complex [Rhodococcus sp.],2OYL_A Endo-glycoceramidase II from Rhodococcus sp.: cellobiose-like imidazole complex [Rhodococcus sp.],2OYL_B Endo-glycoceramidase II from Rhodococcus sp.: cellobiose-like imidazole complex [Rhodococcus sp.],2OYM_A Endo-glycoceramidase II from Rhodococcus sp.: five-membered iminocyclitol complex [Rhodococcus sp.],2OYM_B Endo-glycoceramidase II from Rhodococcus sp.: five-membered iminocyclitol complex [Rhodococcus sp.]
2OSX_A	2.36e-08	18	165	28	163	Chain A, Endoglycoceramidase II [Rhodococcus sp.]
2OSY_A	2.36e-08	18	165	28	163	Chain A, Endoglycoceramidase II [Rhodococcus sp.],2OSY_B Chain B, Endoglycoceramidase II [Rhodococcus sp.]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|I1BTD7|EGCR1_RHIO9	3.10e-151	20	659	15	640	Glucosylceramidase OS=Rhizopus delemar (strain RA 99-880 / ATCC MYA-4621 / FGSC 9543 / NRRL 43880) OX=246409 GN=ERC1 PE=1 SV=1
sp|H1AE12|EGCR1_CRYNH	4.35e-148	19	661	15	653	Glucosylceramidase OS=Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) OX=235443 GN=EGC1 PE=1 SV=1
sp|H1AE13|EGCR1_ASPFM	7.28e-76	23	576	9	563	Glucosylceramidase OS=Neosartorya fumigata OX=746128 GN=egc1 PE=1 SV=1
sp|P40566|EGH1_YEAST	1.27e-69	15	579	2	583	Ergosteryl-beta-glucosidase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=EGH1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000040	0.000000

TMHMM  Annotations     

Start	End
685	707