CAZyme Information: KIX06326.1

Basic Information

• Species: Rhinocladiella mackenziei
• Lineage: Ascomycota; Eurotiomycetes; ; Herpotrichiellaceae; Rhinocladiella; Rhinocladiella mackenziei
• CAZyme ID: KIX06326.1
• CAZy Family: GH5
• CAZyme Description: GPI mannosyltransferase 2 [Source:UniProtKB/TrEMBL;Acc:A0A0D2IT07]

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2466	KN847477|CGC15	277239.36	6.9782

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_RmackenzieiCBS650.93	11418	1442369	36	11382

• Gene Location: location=KN847477:2151594-2160939(+)

Full Sequence      

MNLLSHHTRDHPLLSLFVFFVAWKSLIVLVTLFSPGVGYDTSTSLLSWGEDAHRVVSNTP
DAMQNRWMKFVRWDAVYFTHLAEKGHVYEQEWAFGIGLSTAISWLAQLDLFPDLSEWGIS
TKTPLTLVAGALLSHAAHWLSVVQLWALTTNLLGTDKNSHSALPFYAAALHVFSPAGIFL
SAPYCESAFAFLSMSGFLGFVNAVHHFHQARAFAGCTTMISAALSFSIATLIRSNGILAG
IPYLVDAVTTSLAILFQGLSLTRLCRLMSVVAGGILVGVGLLHPQVLAYKEYCVGRSPAE
RRSWCEATIPSIFSFVQSHYWNVGPFRYWTVSNIPLFLLAAPTLWLLIYSAMDVLRNPQT
ILASSTTTSSASVTTHGRLVAMSLALPQLVLAVLALTSYHVQIITRLCSGYPLWYIWLAT
KAQNHAQCTSSVIRWMVIYALVQAGLYALSPLSSQEEFKTRQRSQLHQRKASPYGVMGLT
NTALTSFIQPLQIMENHKQHMEEVMNGSHGKPGLEISRATSPAGTIAMDNGQTTDAENQV
KRNRPRTYPYFTTLPYPVEDEAQRQKNLAEILKHLYIAIQASDFTPGAVHWTRELRSWLS
LKFDPTKEQRVKLVKLYYELSLAPGIDPGVGERFASMFMLLTKRKHYLRPLKDLTLDWRP
LFRELKVFVLPSESGLMQTTNLKRNIKTLTKITSFAQLYFDPEDVPAMLEELLPHFTMSS
AEGAFVVIGLLNMLLPTAPPPPNRSDLAPQQFLPTFFHLWSLVNRSRTADVAFLDLFSRV
ARDLLPCGHVDFSEFGIFTSEQSTLITTAVLRLLEIPVGQSTSPYSALVDLSAGMGILLD
RDSRKHPVSHHIARWFVMSMSPACLKKKTSVLSLLETLIQAIETFFHPSNSGSWTRTLAQ
LVFYLADFFVMRWNREHNGEMDVPEDRRLDEALRKRFVLCLREVIFMGIYAKSGTAMSYS
LSTLQALAFLEPDLILPGALQRIYPSMQGLVEVHRTISSLRSLQALARTMVRTKGYRCHI
TALLGLALPGIDANDLEKTLYTLSFFANVCYNIPFQDLSQGREDIQGNVLAMEWITGEME
RMEQEGVGVELDYASLNEKDEEMILASSTAGFGEFVTSFLGRVFTLLENLPDAARIRSGS
PEENIVNTLPAAFMPLLASLSPELFDLALNKIVDFVANHVIHQSRDAMAFICNCLCKVDP
EKALAKFVPVLIGAIRTEIDENGAGSTRNAASDVLPRDRGLVWNISMLSMCVVHVGSAVL
KYKQELFDIAVYMQQKCKGMPTVHVSNYVHHLLLNLTVTYTADYALYEPEKLENGVSAEL
WGMAEPPATIRPKWHVPCKEEIDFAVELFQNQAEGALQHLQRLINGTSSIKRDGSGKEWS
DEVSRNLVLLRLLLAGVSVLFDSNGVNEGLTTTAAGLDADVSMSQANGHAPEEGSDAVDT
DASLDGTDETSIKPSFQYPCGQLLHPGDENYRRLHQLRQDVGRVLHDVHDFLTREGEDDV
SSFAPLYTAYRSWFVDVGIERSAHVLDRVTRLLHADEQPYKVSGVRKDYPRSILVRRANV
YHTQRLRHNAAPRPRSELDERLLLDLAESAVSLYTEVRRNAQSAGESALKVVFGARLFVI
PPLLAAFQKAVERNDFPRIKGGLFALLFGSLAKTVGRHWKYTPAVIKTFIEASTADKPSI
QKLCSGGLFQVMDYGRPGDRMAIIDRAVVDPFHPDDDVEDKISKKKAFILSKRANIEKKK
AELAEELIELGRVSHWKKATRVATIVMSLGMRFDHIASDNMIDLITRGTVDPHPGLRGLY
SQGLVALFTMTDVRAVCNHDYANYIQALQDFPAKVEVETHADDPSWTDTYLKAFSQPEAD
VYIDHDYPGWLVWSKNIPGYKANVQSDIKYDEVEWKKRAAIGKLLDREWFVTFFKYLKQE
PRDQSADKFRMASAAMLTYAFELVIRDGLAVATFDDIKEETLSVFEDGSDKHQHRATAEI
LAGLVTSVTDNSIERRTMVWEFAFPIVQRVFSDGLTPENSGYWTTFLHMILQARDPRRAW
PLVEWLTNFRLDMESNAAFKESSKIHLLHQVIMDAGWHFQLDKPITEDFLSHIDHPYKGV
REAMAHTLATITRTRYHESFKDVNELIGAQKSAGSIGKQPYLPTQEFDETMYEVFRRLEK
WRHERVPGQQTPSPYTSGSKTILLWLDSMLSSYECTQLLKYFPDPFTEQFLHMMDVKEDP
ELQSLAYHVFRHLPNIPHRIGEDQRLIDSLIRIGRTSPSWHQRLRVMINMQIVFFRRLFL
LSEESKQTLFDCVAEMLEDTQHEVRAGAATTLSGMIRCSSLELRERMITQLRDRFTKMLI
DNPLPKKPKGQLAGVAGLSSARTSGSNTPTPEAQRLVVVRHAAVLGLGALIQAFPYSSPP
PAWMPDVLVTLSSKAANDPATVGNSVKSIISDFKKTRQDTWHIDVKAFKPEQIEDLSGVL
WKSYFA

Enzyme Prediction     

No EC number prediction in KIX06326.1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT76	18	448	9.2e-100	0.9778869778869779

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
406818	BLM10_mid	0.0	875	1401	1	499	Proteasome-substrate-size regulator, mid region. The ordered regions of the yeast BLM10 or PA200 (human homolog), full-length protein encode 32 HEAT repeat (HR)-like modules, each comprising two helices joined by a turn, with adjacent repeats connected by a linker. Whereas a standard HEAT repeat is composed of ~50 residues, the BLM10 HEAT repeats are highly variable. The length of helices ranges from 8 to 35 residues, turns range from 2 to 87 residues, and linkers range from 1 to 88 residues, with the longest linker, between HR21 and HR22, containing additional secondary structures (two strands and three helices). BLM10_mid is the middle ordered region of the three in BLM10. BLM10 is found to surround the proteasome entry pore in the 1.2 MDa complex of proteasome and BLM10 to form a largely closed dome that is expected to restrict access of potential substrates. Thus Blm10 and PA200 are predominantly nuclear and stimulate the degradation of model peptides, although they do not appear to stimulate the degradation of proteins, recognize ubiquitin, or utilize ATP.
282094	Mannosyl_trans2	1.60e-41	16	424	4	397	Mannosyltransferase (PIG-V). This is a family of eukaryotic ER membrane proteins that are involved in the synthesis of glycosylphosphatidylinositol (GPI), a glycolipid that anchors many proteins to the eukaryotic cell surface. Proteins in this family are involved in transferring the second mannose in the biosynthetic pathway of GPI.
403208	DUF3437	2.39e-38	2378	2465	1	86	Domain of unknown function (DUF3437). This presumed domain is functionally uncharacterized. This domain is found in eukaryotes. This domain is typically between 142 to 163 amino acids in length.
406851	BLM10_N	1.10e-26	513	587	2	81	Proteasome-substrate-size regulator, N-terminal. The ordered regions of the yeast BLM10 or PA200 (human homolog), full-length protein encode 32 HEAT repeat (HR)-like modules, each comprising two helices joined by a turn, with adjacent repeats connected by a linker. Whereas a standard HEAT repeat is composed of ~50 residues, the BLM10 HEAT repeats are highly variable. The length of helices ranges from 8 to 35 residues, turns range from 2 to 87 residues, and linkers range from 1 to 88 residues, with the longest linker, between HR21 and HR22, containing additional secondary structures (two strands and three helices). BLM10_N is the N-terminal ordered region of the three in BLM10. BLM10 is found to surround the proteasome entry pore in the 1.2 MDa complex of proteasome and BLM10 to form a largely closed dome that is expected to restrict access of potential substrates. BLM10 and PA200 are predominantly nuclear and stimulate the degradation of model peptides, although they do not appear to stimulate the degradation of proteins, recognize ubiquitin, or utilize ATP.
227829	COG5542	5.55e-19	72	421	76	366	Mannosyltransferase related to Gpi18 [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QSS49377.1|GT76	2.82e-90	10	448	21	471
CAP97902.1|GT76	1.46e-89	10	447	21	443
QQK45828.1|GT76	2.53e-89	10	449	21	445
QMW40534.1|GT76	1.81e-88	10	447	16	445
QRD82967.1|GT76	6.19e-88	10	447	16	445

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6REY_c	1.53e-99	550	2460	34	1836	Human 20S-PA200 Proteasome Complex [Homo sapiens],6REY_d Human 20S-PA200 Proteasome Complex [Homo sapiens]
6KWX_A	3.01e-99	550	2460	69	1871	cryo-EM structure of human PA200 [Homo sapiens],6KWY_c human PA200-20S complex [Homo sapiens]
4V7O_A6	6.13e-61	1470	2466	22	997	Chain A6, Proteasome activator BLM10 [Saccharomyces cerevisiae],4V7O_A8 Chain A8, Proteasome activator BLM10 [Saccharomyces cerevisiae],4V7O_B5 Chain B5, Proteasome activator BLM10 [Saccharomyces cerevisiae],4V7O_B8 Chain B8, Proteasome activator BLM10 [Saccharomyces cerevisiae]
4V7O_A5	1.82e-33	590	1402	4	793	Chain A5, Proteasome activator BLM10 [Saccharomyces cerevisiae],4V7O_A7 Chain A7, Proteasome activator BLM10 [Saccharomyces cerevisiae],4V7O_B4 Chain B4, Proteasome activator BLM10 [Saccharomyces cerevisiae],4V7O_B7 Chain B7, Proteasome activator BLM10 [Saccharomyces cerevisiae]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|F1MKX4|PSME4_BOVIN	4.96e-100	550	2460	34	1838	Proteasome activator complex subunit 4 OS=Bos taurus OX=9913 GN=PSME4 PE=1 SV=1
sp|F1R2X6|PSM4B_DANRE	3.19e-99	550	2460	19	1818	Proteasome activator complex subunit 4B OS=Danio rerio OX=7955 GN=psme4b PE=3 SV=2
sp|Q5SSW2|PSME4_MOUSE	7.85e-99	550	2460	34	1836	Proteasome activator complex subunit 4 OS=Mus musculus OX=10090 GN=Psme4 PE=1 SV=1
sp|Q14997|PSME4_HUMAN	7.85e-99	550	2460	34	1836	Proteasome activator complex subunit 4 OS=Homo sapiens OX=9606 GN=PSME4 PE=1 SV=2
sp|F1QFR9|PSM4A_DANRE	9.14e-95	550	2460	19	1826	Proteasome activator complex subunit 4A OS=Danio rerio OX=7955 GN=psme4a PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000033	0.000002

TMHMM  Annotations     

Start	End
13	35
127	149
162	184
188	205
212	234
238	260
267	289
328	350
379	401