CAZyme Information: KIX00898.1

Basic Information

• Species: Rhinocladiella mackenziei
• Lineage: Ascomycota; Eurotiomycetes; ; Herpotrichiellaceae; Rhinocladiella; Rhinocladiella mackenziei
• CAZyme ID: KIX00898.1
• CAZy Family: AA3
• CAZyme Description: Rhinocladiella mackenziei CBS 650.93 unplaced genomic scaffold supercont1.8, whole genome shotgun sequence [Source:UniProtKB/TrEMBL;Acc:A0A0D2GRF9]

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1494	KN847482|CGC1	167408.58	4.8720

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_RmackenzieiCBS650.93	11418	1442369	36	11382

• Gene Location: location=KN847482:977284-981824(+)

Full Sequence      

MRTSTLPWRSIGLALLSLTLPSTLAQSSPSINVALETSFSAGPYLVELLETAALENSSSY
FPLLDRIADGVFSDLHTEQELYNKFIDVLKQDGHLSDPESLSSFKFALALHAAAPRIEAH
FQYYNTSVQPYLMAAQDAACPVWVYLDGKQYCSPALDRAQQAVALPDVTDNLPFDRVLGD
VLEAPPSILYADITHPLFGQFHHDVCQTAREGRSTYRVRYRPSMGSGETKPLYVSGYGVE
LVLKRTDYIVIDDRDAHKTGSGNVDPVSLEQVQEDELTDLKPLTTSEVVNLGLNAASFIT
ASSHPLESLLQISSDFPRYSTLLASTNASREFLSEHTANREQFLPSGYNVFWINGVQIEP
RLVNAYSLLDHLRRERRIIGELKEIGLTSAEAINLLSSEVIAEAQANEMPQRFDWRDDVE
ADKVLMWLNDLEQDKRYAAWPSTLPSLFQRVYPGQLPQLARDIHNLIVPVDMTDIKDIEL
VATSLQSLVRRLVPIRIAIIPTGTGETANLYSKLAYHILDTYGLSSLMAFFSDIVSSKKL
SSNPEKSFASAIKGRPLREGREQSSYQDLLTAESLNLRLQARDNYLNRLALGGSRPPFLI
NGVVLPRTDNWFELMSARLYSDLQLIQQAVLQDAILEDTWVPQFFLFQAAARRNEIVFPH
DPKDVKIVDVNYLTANFGEDISSLPRVAGTEAMLLSDRAHLLIIADLDSEAGRVFLSEAA
AFQQKHPETDMLILHNPSSDSPPDQPSRWLYAFVQKSGRDLTIEQLQRSLENESDPLLGE
SDLAEASARFWSSKEGLIGSLGLSAGQTGLWLNGRLLEPSDQPLTAIDLESLLDFERRER
IAPVTTAITGLGLEDRFSDALDLAKVTSLVARSLKSDLPEGLRESAPLIRMDRFKIWQDT
HTSISVSNSNDPIIQIVAVIDPASEVVQPWVPILKTLSQLDGVSVKIFLNPKDRLTELPV
KRFFRQVISAEPRFNADGSLENPNASFTRVPKDTLFNLGMLVPPSWLVAPKSSIYDLDNI
KLSSLGDGENIDALYELEHILIEGHSRDVTNGPPPRGVQLLLGTAQEPHFTDTIVMANLG
YFQFKANPGYWQISLKPGRSSKIFHIDSVGPHGYSAQAGDETTAVALLSFQGLTLFPRLS
RKAGMEEEDVLGSSVLGGALDYLNKGASIASSALSSLGFKSASSSSSSSSVNADINIFSV
ASGHLYERMLNIMMVSVMKHTKHSVKFWFIEQFLSPSFKSTLPHLAKHYGFDYEMVTYKW
PHWLRGQKEKQREIWGYKILFLDVLFPLDLDKVIFVDADQIVRADMMELNKVDLKGAPYG
FTPMCDSRTEMEGFRFWKQGYWENYLRGKPYHISALYVVDLKRFRQLAAGDRLRQQYQAL
SSDPNSLSNLDQDLPNHMQHNLPIHSLSQEWLWCETWCSDESFSSAKTIDLCNNPLTKEP
KLDRARRQVPEWTAYDEEITAVIKAATEEKEEEPVVPEEAHEEVFHDEIKRDEL

Enzyme Prediction     

EC	2.4.1.-:29

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT24	1195	1442	3.7e-135	0.9959677419354839

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
408203	Glyco_transf_24	0.0	1195	1462	1	268	Glucosyltransferase 24. This is the catalytic domain found in UDP-glucose:glycoprotein glucosyltransferase (UGGT). This domain belongs to glucosyltransferase 24 family (GT24) A-type domain. The GT domain displays the expected glycosyltransferase type A (GT-A) fold.
133054	GT8_HUGT1_C_like	0.0	1195	1442	1	248	The C-terminal domain of HUGT1-like is highly homologous to the GT 8 family. C-terminal domain of glycoprotein glucosyltransferase (UGT). UGT is a large glycoprotein whose C-terminus contains the catalytic activity. This catalytic C-terminal domain is highly homologous to Glycosyltransferase Family 8 (GT 8) and contains the DXD motif that coordinates donor sugar binding, characteristic for Family 8 glycosyltransferases. GT 8 proteins are retaining enzymes based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. The non-catalytic N-terminal portion of the human UTG1 (HUGT1) has been shown to monitor the protein folding status and activate its glucosyltransferase activity.
408201	Thioredoxin_14	1.33e-80	413	657	1	248	Thioredoxin-like domain. This is the third out of four TRXL(thioredoxin-like) domains found in UDP-glucose:glycoprotein glucosyltransferase (UGGT).
399437	UDP-g_GGTase	9.61e-64	1043	1151	1	108	UDP-glucose:Glycoprotein Glucosyltransferase. UDP-g_GGTase is an important, central component of the QC system in the ER for checking that glycoproteins are folded correctly. This QC prevents incorrectly folded glycoproteins from leaving the ER.
408199	Thioredoxin_12	7.83e-60	43	228	3	184	Thioredoxin-like domain. This is one of four TRXL(thioredoxin-like) domains found in UDP-glucose:glycoprotein glucosyltransferase (UGGT).

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
UDD64319.1|GT24	0.0	24	1479	24	1473
BAE65008.1|GT24|2.4.1.-	0.0	24	1479	24	1473
CAK39488.1|GT24	0.0	26	1482	27	1475
CAS97678.1|GT24	0.0	26	1482	27	1475
QRD91624.1|GT24	0.0	24	1479	24	1473

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5MU1_A	0.0	27	1470	7	1455	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5MZO_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TRF_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
5NV4_A	0.0	27	1470	7	1455	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
5Y7O_A	0.0	27	1151	2	1123	Crystal structure of folding sensor region of UGGT from Thermomyces dupontii [Thermomyces dupontii],5Y7O_B Crystal structure of folding sensor region of UGGT from Thermomyces dupontii [Thermomyces dupontii]
6TRT_A	0.0	27	1470	7	1455	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TS2_A	0.0	27	1470	7	1221	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_C Chain C, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_D Chain D, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q09140|UGGG_SCHPO	8.75e-285	23	1473	14	1435	UDP-glucose:glycoprotein glucosyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=gpt1 PE=1 SV=2
sp|Q09332|UGGG_DROME	2.98e-230	25	1463	25	1508	UDP-glucose:glycoprotein glucosyltransferase OS=Drosophila melanogaster OX=7227 GN=Uggt PE=1 SV=2
sp|Q6P5E4|UGGG1_MOUSE	9.27e-229	15	1459	28	1520	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Mus musculus OX=10090 GN=Uggt1 PE=1 SV=4
sp|Q9JLA3|UGGG1_RAT	2.68e-227	13	1459	29	1520	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Rattus norvegicus OX=10116 GN=Uggt1 PE=1 SV=2
sp|Q9NYU2|UGGG1_HUMAN	8.50e-226	22	1494	38	1555	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Homo sapiens OX=9606 GN=UGGT1 PE=1 SV=3

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000233	0.999720	CS pos: 25-26. Pr: 0.9799

TMHMM  Annotations     

There is no transmembrane helices in KIX00898.1.