CAZyme Information: KIW06424.1

Basic Information

• Species: Verruconis gallopava
• Lineage: Ascomycota; Dothideomycetes; ; Sympoventuriaceae; Verruconis; Verruconis gallopava
• CAZyme ID: KIW06424.1
• CAZy Family: GH92
• CAZyme Description: Beta-mannosidase [Source:UniProtKB/TrEMBL;Acc:A0A0D2B5C3]

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
985	KN847535|CGC4	111444.60	4.9337

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_VgallopavaCBS43764	9871	N/A	53	9818

• Gene Location: location=KN847535:623084-626187(-)

Full Sequence      

MGKQTSLPTLSRTDEQLLEIRAETRTNPYAKLAWLTTTIIVLSCICKKLLSSLFIHVGQL
ASLGLLVKGLSSHQTVQTITVFDLSKLVWTVGNEALNISVPGSLPSHVHLDLFKARVIND
PYFGLNDFNLRWIALNNWTYSAELPLDQKGSRSTWLLFNGLDTFTSIELCGHHVASTNNQ
FRQYYFDVRNILASCEGVYLLTLNFGSVPAIADEIAAQPGQETWPFGVEGLFEFPNRQFV
RKEQSDFGWDWGPAFVPVGPWLPAYVIQLGPSDLYIRNTMVDIYRRGQLPLIPPDQTQPW
IMNVSLDVIGSIPPNIDLEYRLTDASNITIMSGSLRNVTLSTESITGTTLVPADAVELWW
PNGLGPQTLYSVELVITLGKGVNRLIIASTTKRVGFRTIVLNQEPIRQDQIDRGVAPGNN
WHFEINGHEFYAKGSNFIPPDAFWPRVTRARIQQLFDAVITGNQNMLRVWSSGVYSPDFM
FEIADELGILLWSEFEFGDALYPVDEDFLNNVREEANFQVRRVNHHPSLALWAGGNELEN
LELPMVKRIAPDQFERYLAEYEELFLGVLAPCVFENSRSISYTPSSSGNGWQSLNFSTPQ
PIVQRYDNKTAGCIYGNTDYYQYDSALAFDFSTYPIGRFSNEFGYHSMPSIQSWRQAVAP
EDLHFNSSVIQLRNHHYPPGGLNESNFANSTKGMAEMTIAVQRWYPSPNKTDHAANFSSW
CHATQIFQADFYKSQIMFYRRGSGMPERQLGSLYWQLEDIWQAPTWAGIEYDGRWKVLHY
ITKDIYQNIIITPFNDATTGNFEIYVTSDLWSPANGTASFAWYDWSGNVLDIPIPSFVNF
SVGAINTTRVFQGNMFDMLGSYDPKDVVLRMEIFADGWLPNNKNAQTFNHVNWFHASPLS
QARLVDPGLQIRHQDGFFSVRASRGVAAWVWLDYPEGSVVEFDSNGFWLHAGEERTVGYK
LINGHTGDSWAESVTAQSLFDLTLP

Enzyme Prediction     

EC	3.2.1.25:4

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	104	804	7e-64	0.5930851063829787

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
225789	LacZ	1.77e-41	79	780	13	624	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
407625	Ig_mannosidase	5.61e-15	906	981	1	78	Ig-fold domain. This Ig-like fold domain is found in mannosidase enzymes.
407658	Mannosidase_ig	1.15e-09	800	873	1	71	Mannosidase Ig/CBM-like domain. This domain corresponds to domain 4 in the structure of Bacteroides thetaiotaomicron beta-mannosidase, BtMan2A. This domain has an Ig-like fold.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
UJO11998.1|GH2	0.0	89	984	32	930
SMR45057.1|GH2	0.0	89	985	28	928
SMY20222.1|GH2	0.0	76	985	16	931
SMQ46536.1|GH2	0.0	76	985	13	928
SMR42887.1|GH2	0.0	89	984	28	927

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4CVU_A	0.0	80	985	29	942	Structure of Fungal beta-mannosidase from Glycoside Hydrolase Family 2 of Trichoderma harzianum [Trichoderma harzianum],4UOJ_A Chain A, BETA-MANNOSIDASE GH2 [Trichoderma harzianum],4UOJ_B Chain B, BETA-MANNOSIDASE GH2 [Trichoderma harzianum]
6DDT_A	1.19e-111	89	947	18	833	mouse beta-mannosidase (MANBA) [Mus musculus],6DDU_A mouse beta-mannosidase bound to beta-D-mannose (MANBA) [Mus musculus]
2VJX_A	2.54e-82	89	809	15	672	Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]
2JE8_A	2.63e-82	89	809	17	674	Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JE8_B Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
7OP6_A	2.68e-82	89	809	17	674	Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP6_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_A Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q4WMS9|MANBA_ASPFU	0.0	81	981	24	921	Beta-mannosidase A OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=mndA PE=3 SV=1
sp|B8NP78|MANBA_ASPFN	0.0	83	984	25	912	Beta-mannosidase A OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=mndA PE=3 SV=1
sp|Q0CI48|MANBA_ASPTN	0.0	81	983	25	929	Beta-mannosidase A OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=mndA PE=3 SV=2
sp|Q2UN00|MANBA_ASPOR	0.0	83	984	25	912	Beta-mannosidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=mndA PE=3 SV=1
sp|A1CTM5|MANBA_ASPCL	0.0	81	981	24	927	Beta-mannosidase A OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107) OX=344612 GN=mndA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.999283	0.000684

TMHMM  Annotations     

There is no transmembrane helices in KIW06424.1.