CAZyme Information: KIW05717.1

Basic Information

• Species: Verruconis gallopava
• Lineage: Ascomycota; Dothideomycetes; ; Sympoventuriaceae; Verruconis; Verruconis gallopava
• CAZyme ID: KIW05717.1
• CAZy Family: GH64
• CAZyme Description: hypothetical protein, variant 1

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1507	KN847537|CGC2	169281.75	4.8480

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_VgallopavaCBS43764	9871	N/A	53	9818

• Gene Location: location=KN847537:702372-707020(+)

Full Sequence      

MRVPSWLLPAELLLLVGSLHLLPIAVDAASQSVNIGLKASFSSAPYLVELLETAAEENEG
VYFKILDRIANGYFQSAASDKEYYERFIQLLEEDELITDPAALSSFELALAVHSAAPRIE
AQYQYYKTSIEPLLQRSGAKDCKTWLEFAGKQFCEPQLEKEDATIQDLNIRGSGALPFDR
WERQNKTLLPATLYADILSPDFGKFHTLMRDAAKEGKVQYRVRHKPSQTETHALAMSGFG
AELALKKTDYIVIDDRDKDVKTEEKATEQDLEAADEEIADLTPLSSSELTDLAMKTASYI
MTSEFPLKRLLKVTQDFPKYSSFLASQEVSALFRKEHVGNRELLLPSGFNVIWLNGLQFD
PRKVDAFSLIDHMRRERKLIASFKDLGFTNAEAIQLLTHPALAESQQDAANSDITRYDWR
DDIEGGDVIIWLNDIEADKRYEDWPSNLMSFLQRTYPGQLPMVRRDLHNMILPVDFSGAR
SVGPIVQTVQDFVKRGIPVRFGLVPLSHSESAAKQAKVVYHIYEYYGLSALMQYLESALT
ANQFGDPNEGIFQAVIEDRKIKAGFEPRSLQHVLEPDDTDERVRKAQKYLARLGANSESP
PLFINGVPIKNNDDWLQSFSQRISIDMRMVQRKVWEGAIDEEMWLPGMFLEDASLKRNAL
VIPEDEKQIRLVSLLKAYEGKNADLVKSLPTISASDDTPAEDKSQLVVIGDFTSEHGLRL
LMEAIAFRQQHADVEVVLLDNNANTKSRLGHVIQELRVDESGLQDFSEKLAGLLSSAERI
DADYPVPWGDVTPLIEIFGFTTGEAGLVLNGRKVGPIPSDEVFAKDDFEALYAFELKKRI
MPARTAIKHLNFGEKVGNALNNAKLFSTLALSTVSDIPEGIFELPPPIRTRLFYDWNATN
TAITVGDNSTATIQIVAAIDPAAEIAQQWVPILNVLSQLDGVYLRIFLNPIEKLKELPIK
RFYRYALNTKPTFDERGEATALGVKFEGIPKNALLTAGLNVPPSWLVAPTDAVHDLDNIK
LSSLSAGTNIEATYTLENILIEGHSRDVTTGLAPRGAQLALSTAKDQHFADTLIMANLGY
FQFKANPGYYNISLLPGLSKKIFNIDSVGAFGYDAQPGDESTEVTLMSFSGVTLYPRLSR
KPGMEEEDVLGTPKTSAADVVADAAADLVSDVAGMVDTLLSKVGVKLPHAAEVVTTSPHA
DINIFSVASGHLYERMLNIMMVSVMKHTNHTVKFWFIEQFLSSSFKTFLPTLAEEYGFKY
EMVTYKWPHWLRAQKEKQREIWGYKILFLDVLFPLDLDKVIFVDADQIVRTDMIELVNID
LKGAPYGFPPMGDSRTEMEGFRFWKQGYWKNFLKGLPYHISALYVVDLKRFRQIAAGDRL
RQQYHQLSADPGSLSNLDQDLPNSMQMVIPIYTLPKEWLWCETWCSDEDLATAKTIDLCN
NPMTKEPKLDRARRQIPEWTVYDEEIAALARRTKGEVPKGDEQERLQKDEEAKEGKRAGS
APEHNEL

Enzyme Prediction     

EC	2.4.1.-:29

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT24	1202	1449	5.1e-131	0.9959677419354839

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
408203	Glyco_transf_24	0.0	1202	1469	1	268	Glucosyltransferase 24. This is the catalytic domain found in UDP-glucose:glycoprotein glucosyltransferase (UGGT). This domain belongs to glucosyltransferase 24 family (GT24) A-type domain. The GT domain displays the expected glycosyltransferase type A (GT-A) fold.
133054	GT8_HUGT1_C_like	3.90e-177	1202	1449	1	248	The C-terminal domain of HUGT1-like is highly homologous to the GT 8 family. C-terminal domain of glycoprotein glucosyltransferase (UGT). UGT is a large glycoprotein whose C-terminus contains the catalytic activity. This catalytic C-terminal domain is highly homologous to Glycosyltransferase Family 8 (GT 8) and contains the DXD motif that coordinates donor sugar binding, characteristic for Family 8 glycosyltransferases. GT 8 proteins are retaining enzymes based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. The non-catalytic N-terminal portion of the human UTG1 (HUGT1) has been shown to monitor the protein folding status and activate its glucosyltransferase activity.
408201	Thioredoxin_14	1.94e-93	417	661	1	248	Thioredoxin-like domain. This is the third out of four TRXL(thioredoxin-like) domains found in UDP-glucose:glycoprotein glucosyltransferase (UGGT).
399437	UDP-g_GGTase	1.45e-60	1042	1150	1	108	UDP-glucose:Glycoprotein Glucosyltransferase. UDP-g_GGTase is an important, central component of the QC system in the ER for checking that glycoproteins are folded correctly. This QC prevents incorrectly folded glycoproteins from leaving the ER.
408200	Thioredoxin_13	1.37e-56	276	410	1	135	Thioredoxin-like domain. This is the second out of four TRXL(thioredoxin-like) domains found in UDP-glucose:glycoprotein glucosyltransferase (UGGT).

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QDS71787.1|GT24	0.0	1	1507	1	1504
AQQ13387.1|GT24	0.0	29	1476	24	1482
AOZ60530.1|GT24	0.0	28	1476	23	1482
CAE6999215.1|GT24	0.0	1	1507	1	1508
QRC95338.1|GT24	0.0	1	1507	1	1505

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5MU1_A	0.0	28	1479	6	1457	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5MZO_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TRF_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
5NV4_A	0.0	28	1479	6	1457	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
5Y7O_A	0.0	29	1152	2	1125	Crystal structure of folding sensor region of UGGT from Thermomyces dupontii [Thermomyces dupontii],5Y7O_B Crystal structure of folding sensor region of UGGT from Thermomyces dupontii [Thermomyces dupontii]
6TRT_A	0.0	28	1479	6	1457	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TS2_A	0.0	28	1479	6	1223	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_C Chain C, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_D Chain D, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q09140|UGGG_SCHPO	1.48e-262	24	1469	13	1424	UDP-glucose:glycoprotein glucosyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=gpt1 PE=1 SV=2
sp|Q09332|UGGG_DROME	4.86e-227	27	1472	24	1510	UDP-glucose:glycoprotein glucosyltransferase OS=Drosophila melanogaster OX=7227 GN=Uggt PE=1 SV=2
sp|Q9NYU2|UGGG1_HUMAN	4.45e-218	25	1480	39	1534	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Homo sapiens OX=9606 GN=UGGT1 PE=1 SV=3
sp|Q9JLA3|UGGG1_RAT	1.15e-216	27	1469	41	1523	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Rattus norvegicus OX=10116 GN=Uggt1 PE=1 SV=2
sp|Q6P5E4|UGGG1_MOUSE	3.14e-216	27	1469	41	1523	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Mus musculus OX=10090 GN=Uggt1 PE=1 SV=4

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000206	0.999785	CS pos: 28-29. Pr: 0.9711

TMHMM  Annotations     

There is no transmembrane helices in KIW05717.1.