CAZyme Information: KIS70973.1

Basic Information

• Species: Ustilago maydis
• Lineage: Basidiomycota; Ustilaginomycetes; ; Ustilaginaceae; Ustilago; Ustilago maydis
• CAZyme ID: KIS70973.1
• CAZy Family: GT2
• CAZyme Description: unspecified product

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
749		80098.51	6.2420

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_Umaydis521	6910	237631	145	6765

• Gene Location: location=CM003141:210813-213062(-)

Full Sequence      

MAIHKRTTASQLLISIVGVLALFSNLTTVSAQTSRITCSASSQCPESSPCCSSEGVCGSG
AMQCAGGCNPMASFKPSSCLPNPVCRSRNMTIKPSDYNNKDTFMPILEYNGNASSPFTLD
SGTLGAGPEGVLLQLKAAQQAKISSTDYFMYGYVEATLRHDARQGVVAAFILMSDVKDEV
DWEFVADGSVGMTNYFRLGVPVLDHGVNVTTPNFNTSEWHTYGLNWTSSQLQWTIDGRVV
RTLSRAEAGADYPQTPSRIQLSTWAGGNDTNPEGTIAWAGGPIDWNSPEYKQNGFYSQEI
KQFNVQCASIDSLDLTSIPAENSSNGSNPGSANVTSFGYTGANSTSSLGPAFGTSTDPLR
ILSDPGADGIPGYPGYGAADTGPSGKGKQDSKGSSNDKNDHDGSKDGDAHSSSVPTSDAL
KYALPISGAIVGLAALWAIIAFVRSRHLKAPIINAIGVTGVNDPRFTAPLYGGRYGKASN
SLATSAYQDVEHEPASIYRNEPNMNLMMGGGIMSDVGVSHLQRKTTGGSKVSRSSSRGVA
DRLLRNQSRAQRYQQLDATLEEEDYDQVTGYPVSKRFRSNAGVASYLTGADACQGRGYEE
AVQEEEEKNDFDRPATYSEVHSYYDTPGSSPVKSNAHAGRGFYGPAATDTSFEACRLESP
QRRALPITPYTPNYAAVSVSAPARPRPSGRMYDTPNVNYYPTPVMTQSEGYQPNARLSYS
ANPRVQASQPVEGCYRGVYAPISPPRRHS

Enzyme Prediction     

No EC number prediction in KIS70973.1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH16	134	284	9.3e-53	0.9022988505747126

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
185692	GH16_fungal_CRH1_transglycosylase	5.95e-85	108	309	2	203	glycosylphosphatidylinositol-glucanosyltransferase. Group of fungal GH16 members related to Saccharomyces cerevisiae Crh1p. Chr1p and Crh2p are transglycosylases that are required for the linkage of chitin to beta(1-3)glucose branches of beta(1-6)glucan, an important step in the assembly of new cell wall. Both have been shown to be glycosylphosphatidylinositol (GPI)-anchored. A third homologous protein, Crr1p, functions in the formation of the spore wall. They belongs to the family 16 of glycosyl hydrolases that includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.
395585	Glyco_hydro_16	4.24e-36	133	280	13	168	Glycosyl hydrolases family 16.
185683	Glyco_hydrolase_16	4.70e-21	144	285	60	206	glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.
211314	ChtBD1_GH16	1.11e-19	36	83	1	47	Hevein or Type 1 chitin binding domain subfamily that co-occurs with family 16 glycosyl hydrolases. This subfamily includes Saccharomyces cerevisiae Utr2p, also known as Crh2p, which participates in the cross-linking of chitin to beta(1-3)- and beta(1-6) glucan in the cell wall, and S. cerevisiae Crr1p, a putative transglycosidase which is needed for proper spore wall assembly. ChtBD1 is a lectin domain found in proteins from plants and fungi that bind N-acetylglucosamine, plant endochitinases, wound-induced proteins, and the alpha subunit of Kluyveromyces lactis killer toxin. This domain is involved in the recognition and/or binding of chitin subunits; it typically occurs N-terminal to glycosyl hydrolase domains in chitinases, together with other carbohydrate-binding domains, or by itself in tandem-repeat arrangements.
185684	GH16_lichenase	1.48e-17	141	288	58	200	lichenase, member of glycosyl hydrolase family 16. Lichenase, also known as 1,3-1,4-beta-glucanase, is a member of glycosyl hydrolase family 16, that specifically cleaves 1,4-beta-D-glucosidic bonds in mixed-linked beta glucans that also contain 1,3-beta-D-glucosidic linkages. Natural substrates of beta-glucanase are beta-glucans from grain endosperm cell walls or lichenan from the Islandic moss, Cetraria islandica. This protein is found not only in bacteria but also in anaerobic fungi. This domain includes two seven-stranded antiparallel beta-sheets that are adjacent to one another forming a compact, jellyroll beta-sandwich structure.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CDR88212.1|CBM18|GH16_19	4.39e-313	1	748	1	746
CBQ70290.1|CBM18|GH16_19	6.00e-310	1	746	1	731
SJX60964.1|CBM18|GH16_19	4.39e-296	1	746	1	725
SHO79225.1|CBM18|GH16	1.16e-56	30	447	12	412
ATY61824.1|CBM18|GH16_19	3.56e-54	29	370	10	334

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6IBU_A	2.45e-32	110	308	28	224	Apo Crh5 transglycosylase [Aspergillus fumigatus Af293],6IBU_B Apo Crh5 transglycosylase [Aspergillus fumigatus Af293],6IBW_A Crh5 transglycosylase in complex with NAG [Aspergillus fumigatus Af293],6IBW_B Crh5 transglycosylase in complex with NAG [Aspergillus fumigatus Af293]
3D6E_A	8.67e-10	145	266	53	173	Crystal structure of the engineered 1,3-1,4-beta-glucanase protein from Bacillus licheniformis [Bacillus licheniformis],3D6E_B Crystal structure of the engineered 1,3-1,4-beta-glucanase protein from Bacillus licheniformis [Bacillus licheniformis]
5SV8_A	1.31e-06	177	264	87	171	Crystal Structure of the catalytic nucleophile and surface cysteine mutant of VvEG16 in complex with a xyloglucan oligosaccharide [Vitis vinifera]
5DZE_A	7.72e-06	177	264	86	170	Crystal Structure of the catalytic nucleophile mutant of VvEG16 in complex with cellotetraose [Vitis vinifera]
5DZF_A	7.81e-06	177	264	87	171	Crystal Structure of the catalytic nucleophile mutant of VvEG16 in complex with a mixed-linkage glucan octasaccharide [Vitis vinifera],5DZF_B Crystal Structure of the catalytic nucleophile mutant of VvEG16 in complex with a mixed-linkage glucan octasaccharide [Vitis vinifera]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q4WI46|CRF2_ASPFU	1.68e-51	27	307	16	300	Probable glycosidase crf2 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=crf2 PE=1 SV=1
sp|Q5AJC0|UTR2_CANAL	1.54e-44	20	302	11	297	Extracellular glycosidase UTR2 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=UTR2 PE=1 SV=1
sp|P32623|CRH2_YEAST	7.24e-38	16	307	8	300	Probable glycosidase CRH2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=UTR2 PE=1 SV=3
sp|Q8J0P4|CRF1_ASPFU	3.03e-30	110	308	49	245	Probable glycosidase crf1 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=crf1 PE=1 SV=2
sp|Q75A41|CRR1_ASHGO	1.08e-27	36	305	29	359	Probable glycosidase CRR1 OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) OX=284811 GN=CRR1 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000260	0.999740	CS pos: 31-32. Pr: 0.9685

TMHMM  Annotations     

Start	End
12	31