CAZyme Information: KID70237.1

Basic Information

• Species: Metarhizium anisopliae
• Lineage: Ascomycota; Sordariomycetes; ; Clavicipitaceae; Metarhizium; Metarhizium anisopliae
• CAZyme ID: KID70237.1
• CAZy Family: GT20
• CAZyme Description: Glycoside hydrolase, subgroup, catalytic core

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1150	AZNF01000002|CGC7	126750.68	6.9767

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_ManisopliaeARSEF549	10891	1276135	0	10891

• Gene Location: location=AZNF01000002:4170615-4174726(-)

Full Sequence      

MRQCWTEEELLRDLTPIPTGVPQNDPWALPLIDGCPRNCVEAGPDSANWTQLYDQNHLSL
CKYPLLFAFNVHNSPSRYSTIRVCALNGGDGKRSTSAVVVPEKDGDAAPPAARLPTASFS
ECGAGESTTKATVKAGFDVLSENTRSDAMSAANFLISHLSSNTACGNIIIFVKFGVALVS
LYAGADLKKDRAAELLHKYCTAKTSTTIGIFASEGNDIGRVQEALRTWALGECLPSQGMR
VVEKSVELRILLSTAQRNASNSTQVEGERSRRSLASLLTARGECRAIQVDDGDSCASLAS
KCGISGAQFTKYNSDSKLHTCSTLMKGRWVCCSGGDLPDKRPKTQADGTCYTLKVDSGDS
CWGITTSYGITTDELNKWNKETWGWQGCAGLQKDQIICLSSGNSPMPATVDGVACGPQKP
GTRKPSGSFTGKDLAKLNPCPLNACCSGWGYCGTTAEFCTESLADTGAPGTHKPGTNGCI
SNCNMNIIRSPEPPASFLSIGYFEGYGLTKICDRVDIRTIDLKKYTHIHFAFATVTANTF
DVNMGPTLNQFYYFKQLTGVKKILAFGGWTFSTDPATYGIFRTGVSPAHRLQMAQSIAKF
IIDNELDGVDIDWEYPAAPDLPDIPAGDPAEGINYLKFLITLKQLLPTDKTLSFAAPASF
WYLKGFPIAQIMKVVDYVVYMTYDLHGQWDYGNKWSTPGCPAGNCLRSHVNMTETLNALS
MITKAGAPSNKIAVGVTSYGRSFKMATPGCTGPMCTYTGPKSQAAKGRCTDTAGYISQNE
IDYIIKNNPSATKLYDDASLSNILIYDETEWVAYMSADIKKKRRHLYRALNFLGTSDWAI
SLDGTDLVKPEPSTMSPVGPSLARPGVMDLLIHDSCKIKGRLEKVQEAWFEAGDISKAPY
EWSRWNRYQKAMDNYFGKRSGQVPIIGTDKLWENFKRHSSVHFGRSLYGFKIMNMYTYIF
CDEADLPKKVHEDYNVVCVNAKKGNKHVAAVTYRYPGTWFWSEYYILLCPGFLDELPNQP
KFTSLKDLAYEADSFPLLQQQINRWSSGVRAVTMYHEVAHFQDVSDPECGKIEFYNPREI
VVKARSGSRIEYEELLRNAHSWALAAVSMWMMKRWPNIDVPLPRNMKPQSEGNDDDNDGA
FADHVDPDSE

Enzyme Prediction     

EC	3.2.1.14:3	3.2.1.14:3

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH18	500	845	4.1e-50	0.9324324324324325

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
119357	GH18_zymocin_alpha	0.0	499	843	2	345	Zymocin, alpha subunit. Zymocin is a heterotrimeric enzyme that inhibits yeast cell cycle progression. The zymocin alpha subunit has a chitinase activity that is essential for holoenzyme action from the cell exterior while the gamma subunit contains the intracellular toxin responsible for G1 phase cell cycle arrest. The zymocin alpha and beta subunits are thought to act from the cell's exterior by docking to the cell wall-associated chitin, thus mediating gamma-toxin translocation. The alpha subunit has an eight-stranded TIM barrel fold similar to that of family 18 glycosyl hydrolases such as hevamine, chitolectin, and chitobiase.
214753	Glyco_18	3.65e-55	500	843	3	334	Glyco_18 domain.
395573	Glyco_hydro_18	1.60e-50	500	843	3	307	Glycosyl hydrolases family 18.
119365	GH18_chitinase	7.11e-43	499	788	1	310	The GH18 (glycosyl hydrolases, family 18) type II chitinases hydrolyze chitin, an abundant polymer of N-acetylglucosamine and have been identified in bacteria, fungi, insects, plants, viruses, and protozoan parasites. The structure of this domain is an eight-stranded alpha/beta barrel with a pronounced active-site cleft at the C-terminal end of the beta-barrel.
119349	GH18_chitinase-like	7.17e-34	500	685	2	178	The GH18 (glycosyl hydrolase, family 18) type II chitinases hydrolyze chitin, an abundant polymer of beta-1,4-linked N-acetylglucosamine (GlcNAc) which is a major component of the cell wall of fungi and the exoskeleton of arthropods. Chitinases have been identified in viruses, bacteria, fungi, protozoan parasites, insects, and plants. The structure of the GH18 domain is an eight-stranded beta/alpha barrel with a pronounced active-site cleft at the C-terminal end of the beta-barrel. The GH18 family includes chitotriosidase, chitobiase, hevamine, zymocin-alpha, narbonin, SI-CLP (stabilin-1 interacting chitinase-like protein), IDGF (imaginal disc growth factor), CFLE (cortical fragment-lytic enzyme) spore hydrolase, the type III and type V plant chitinases, the endo-beta-N-acetylglucosaminidases, and the chitolectins. The GH85 (glycosyl hydrolase, family 85) ENGases (endo-beta-N-acetylglucosaminidases) are closely related to the GH18 chitinases and are included in this alignment model.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QLI63815.1|CBM18|GH18	0.0	373	1148	2	777
AIT18913.1|CBM18|CBM50|GH18	0.0	22	1123	50	1141
ATY59188.1|CBM18|CBM50|CBM66|GH18	1.94e-257	35	1123	33	1158
ALI93563.1|CBM18|CBM50|GH18	6.22e-253	24	842	61	903
ANH56471.1|CBM18|CBM50|GH18	6.22e-253	24	842	61	903

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6JM7_A	2.10e-26	514	843	22	350	Crystal structure of Ostrinia furnacalis Group IV chitinase [Ostrinia furnacalis],6JMB_A Chain A, ofchtiv-allosamidin [Ostrinia furnacalis]
6JM8_A	2.26e-25	514	843	22	350	Crystal structure of Ostrinia furnacalis Group IV chitinase [Ostrinia furnacalis]
5WUS_A	7.43e-24	520	843	26	342	Crystal structure of a insect group III chitinase (CAD2) from Ostrinia furnacalis [Ostrinia furnacalis],5WVH_A Crystal structure of an insect group III chitinase complex with (GlcNAc)6 (CAD2-(GlcNAc)6) from Ostrinia furnacalis [Ostrinia furnacalis]
5WVF_A	7.57e-23	520	843	26	342	Crystal structure of a mutant insect group III chitinase (CAD2-E647L) from Ostrinia furnacalis [Ostrinia furnacalis],5WVG_A Crystal structure of a mutant insect group III chitinase complex with (GlcNAc)5 (CAD1-E647L-(GlcNAc)5) from Ostrinia furnacalis [Ostrinia furnacalis]
3FXY_A	3.73e-22	526	843	31	344	Acidic Mammalian Chinase, Catalytic Domain [Homo sapiens],3FXY_B Acidic Mammalian Chinase, Catalytic Domain [Homo sapiens],3FXY_C Acidic Mammalian Chinase, Catalytic Domain [Homo sapiens],3FXY_D Acidic Mammalian Chinase, Catalytic Domain [Homo sapiens],3FY1_A The Acidic Mammalian Chitinase catalytic domain in complex with methylallosamidin [Homo sapiens],3FY1_B The Acidic Mammalian Chitinase catalytic domain in complex with methylallosamidin [Homo sapiens],3RM4_A AMCase in complex with Compound 1 [Homo sapiens],3RM4_B AMCase in complex with Compound 1 [Homo sapiens],3RM8_A AMCase in complex with Compound 2 [Homo sapiens],3RM8_B AMCase in complex with Compound 2 [Homo sapiens],3RM9_A AMCase in complex with Compound 3 [Homo sapiens],3RM9_B AMCase in complex with Compound 3 [Homo sapiens],3RME_A AMCase in complex with Compound 5 [Homo sapiens],3RME_B AMCase in complex with Compound 5 [Homo sapiens]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P09805|KTXA_KLULA	3.52e-94	282	839	191	708	Killer toxin subunits alpha/beta OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) OX=284590 PE=1 SV=1
sp|Q95M17|CHIA_BOVIN	1.38e-22	526	843	52	365	Acidic mammalian chitinase OS=Bos taurus OX=9913 GN=CHIA PE=1 SV=1
sp|Q6RY07|CHIA_RAT	3.32e-21	526	843	52	365	Acidic mammalian chitinase OS=Rattus norvegicus OX=10116 GN=Chia PE=2 SV=1
sp|Q91XA9|CHIA_MOUSE	5.90e-21	526	843	52	365	Acidic mammalian chitinase OS=Mus musculus OX=10090 GN=Chia PE=1 SV=2
sp|Q9W5U2|CHI10_DROME	1.27e-20	520	843	989	1310	Probable chitinase 10 OS=Drosophila melanogaster OX=7227 GN=Cht10 PE=2 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.999468	0.000529

TMHMM  Annotations     

There is no transmembrane helices in KID70237.1.