CAZyme Information: KID68303.1

Basic Information

• Species: Metarhizium anisopliae
• Lineage: Ascomycota; Sordariomycetes; ; Clavicipitaceae; Metarhizium; Metarhizium anisopliae
• CAZyme ID: KID68303.1
• CAZy Family: GH75
• CAZyme Description: beta-mannosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
994	AZNF01000003|CGC13	113346.25	6.8700

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_ManisopliaeARSEF549	10891	1276135	0	10891

• Gene Location: location=AZNF01000003:1275554-1278907(-)

Full Sequence      

MSPEATFEPRRRHLRARIICQGIEYHNQSSMETHAAIPGLEPELSLPNPCWYIHRQLRKP
STVRSVAPSIVLLQVARQSPLVTDLIAHPPRHPPTPNAMFSPHAVGNIHPRIKSADVDYG
KRRPAMWGTVTLPWGTWRLRETIGFRPLRFTTITPWGIPDPFVDMNERAVQWIGEKVWQY
RVSFPAPKASSASAVTDLVFEGLDTFATVLLNGREILKTDNMFVSYRVNINEHIKPDGNN
ILEIVFDSALIRGREIVKEHSHEHSFLVRQTEAGRVAVRKAQYNWGWDWGPIIMTAGPWK
PIYLEQYTARVDDVWAQNELDSDLEAVTGTIYTNVVGDFHPDDRIAISLTLEGRKVLEEL
APVPRNGKYAVPFKIANPQLWYPRSYGKQTRYKLQASIVRSGAELHSTSKLIGFRRAELI
QEPDAHGKSFYFRINNVDVFGGGSCWIPADSYLSQISPARYHDWIKLMAEGNQVMVRVWG
GGIYEDDAFLDACDEFGVLIWHDFQFACASYPTYPSYMKTLEMEVRQQLRRLRCHPAVVA
WAGNNEDYQVQERYRLDYDFENKDPESWLKSTFPARYIYEHFLPELVKQEDPFMIYHPAS
PWGDGKPTADPTVGDIHQWNLWHGAVNKYQEVSLLGGRFVSEFGMEAYPHLSTVRRMTTH
SSQLYPGSMTIDFHNKGIFNERRMTTYVSENFRLKYDLPSYIHLTQVVQAEAMRAAYKTW
RRDWGTSGDRKCGGVLVWQLNDCWPTMSWAVVDYYLVKKPAYYAISRALRPLDVGVSRTY
HDWTQTGYYIDENSTLCTGQVDQTLPARESTFDVWIASSRVQAVNAKVTVRFISIRSGRD
VSESITASVSASANATTTVFSSKPLKPSIPHHDDYTIPFDVTQYDPYVVYTTLSVDGAVV
GTDAAWPDPIKFLDLSNRGISFAISENRVTVSSDRPVKGFVFEEVQGMKLSDNGFDIMPG
EQHVVTVEGPVSADKLRWTHIDADDASMEINTRR

Enzyme Prediction     

EC	3.2.1.25:3

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	126	805	3e-79	0.6223404255319149

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
225789	LacZ	3.38e-65	158	822	47	679	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
407658	Mannosidase_ig	5.85e-10	810	911	1	90	Mannosidase Ig/CBM-like domain. This domain corresponds to domain 4 in the structure of Bacteroides thetaiotaomicron beta-mannosidase, BtMan2A. This domain has an Ig-like fold.
395572	Glyco_hydro_2	9.64e-09	309	415	1	106	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
407625	Ig_mannosidase	2.18e-06	925	977	13	68	Ig-fold domain. This Ig-like fold domain is found in mannosidase enzymes.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QLI66193.1|GH2	0.0	158	994	44	880
UKZ88363.1|GH2	0.0	158	991	44	888
UKZ77816.1|GH2	0.0	158	991	44	886
UKZ51991.1|GH2	0.0	158	991	44	886
QYS99024.1|GH2	0.0	158	991	44	886

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5N6U_A	1.54e-128	158	776	62	660	Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_B Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_C Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_D Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12]
2VJX_A	2.42e-107	158	970	44	819	Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]
2JE8_A	2.53e-107	158	970	46	821	Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JE8_B Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
7OP6_A	2.59e-107	158	970	46	821	Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP6_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_A Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482]
2WBK_A	6.66e-107	158	970	44	819	Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482],2WBK_B Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q4WAH4|MANBB_ASPFU	3.78e-281	158	969	46	830	Beta-mannosidase B OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=mndB PE=3 SV=1
sp|B0YBU9|MANBB_ASPFC	1.52e-280	158	969	46	830	Beta-mannosidase B OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=mndB PE=3 SV=1
sp|A1D911|MANBB_NEOFI	1.52e-280	158	969	46	830	Beta-mannosidase B OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=mndB PE=3 SV=1
sp|Q5B7W2|MANBB_EMENI	2.84e-280	158	974	46	833	Beta-mannosidase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=mndB PE=1 SV=2
sp|A1CGA8|MANBB_ASPCL	1.73e-279	158	974	46	835	Beta-mannosidase B OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107) OX=344612 GN=mndB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000051	0.000002

TMHMM  Annotations     

There is no transmembrane helices in KID68303.1.