CAZyme Information: KDQ25670.1

Basic Information

• Species: Pleurotus ostreatus
• Lineage: Basidiomycota; Agaricomycetes; ; Pleurotaceae; Pleurotus; Pleurotus ostreatus
• CAZyme ID: KDQ25670.1
• CAZy Family: CBM67|CBM67
• CAZyme Description: glycoside hydrolase family 35 protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1038		113284.14	6.4630

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_PostreatusPC15	12460	1137138	164	12296

• Gene Location: location=KL198010:1537527-1541727(-)

Full Sequence      

MDLYLPAERQARRVWPILLLISFLATVFTSGTPFSWASLDTANHLFEIVSRSPSTPDLTE
RALSQQVRFDNFSLFVRDQRVFIHSGEFHTFRLPVPSLWPDILQKAKAAGLNAISVYTHM
GLINPSRGVVDFDGFRALQPLFDAAKDAGIFIVLRPGPYINAETTAGGIAHWATSEVAGN
LRTGAADWKAAWQDYIQGIIKVTVPNQITEGGPVIDLPDNEFQQNPEGHAQYFVDLEEAY
RASSIVVPLTYNDPGQGRNFINGTGAVDLYGLDSYPQGFDCSNPTVWHGVTTNYQQYHAS
ANPSQPWYIPEFQGGSFDAWGPTAPGYAPCRTLTGADFQSVFNLQLWASNAKLINYYMFY
GGTSWGGIAFPGVYTSYDYGASLEESRQLTSKFDELKRQGLFLRSSPEFYKTDWIGDTST
GATSATSTAVFATLLRNPDTKAGFYILRQTDATSTAVTNFKLNVSTSAGNILIPNVASAI
TLGGRQSKVVVTDYAFGSSRLLYSTAQIFFAGAIDGRDVLFLFGDSTQEHELVLTLAGTP
RGLRPQSSAVHFANSSLSSSRTTVSFLPGIEGLVTVWDSDKQLVLFSDTATAATFWAPTI
AGKASDPLKNFWQIGTNTTILVGGPHLVRSASISGSQLDLKGDLSESVRLSVIAPSNVRS
ITWNGQRVDADVQTSSQLTFRGGFVSSLKATTTGIKIPKLEKWKFSDSLPEIRAGFSDQK
WAVANHTSTNIPFKPYYGDGRILYGCDYGFCENIVLWRGHFNATGSEKSVNLSINGGEAF
AASVWLNNVFLKTSFGNSTNNRNTLEETDDKFTFPPGALLKGDNVLTIVQDNMGLNETRG
LDTNSSKGPRGVRGFQLDTGNFTEWRVQGKVGGYTNFPDKVRGIMNEGGLFGERQGWHLP
GFDTSAWKERDLASGLPGSAAGVGFFVTKFDLNIPRGFDAFLSFTFNEPLGQPYRALLFV
NGWQMGKRVGNLGPQAKFPVHEGILNYRGRNTIAVALWAMESNVPITPDLQLTFDGVVEG
GVGNVKVNNPAWSSVGRV

Enzyme Prediction     

EC	-	-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH35	74	398	1.8e-72	0.9837133550488599

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
396048	Glyco_hydro_35	1.59e-68	73	398	1	313	Glycosyl hydrolases family 35.
402180	BetaGal_dom2	3.84e-45	410	592	2	178	Beta-galactosidase, domain 2. This is the second domain of the five-domain beta-galactosidase enzyme that altogether catalyzes the hydrolysis of beta(1-3) and beta(1-4) galactosyl bonds in oligosaccharides as well as the inverse reaction of enzymatic condensation and trans-glycosylation. This domain is made up of 16 antiparallel beta-strands and an alpha-helix at its C-terminus. The fold of this domain appears to be unique. In addition, the last seven strands of the domain form a subdomain with an immunoglobulin-like (I-type Ig) fold in which the first strand is divided between the two beta-sheets. In penicillin spp this strand is interrupted by a 12-residue insertion which forms an additional edge-strand to the second beta-sheet of the sub-domain. The remainder of the second domain forms a series of beta-hairpins at its N-terminus, four strands of which are contiguous with part of the Ig-like sub-domain, forming in total a seven-stranded antiparallel beta-sheet. This domain is associated with family Glyco_hydro_35, pfam01301, which is N-terminal to it, but itself has no metazoan members.
198097	BetaGal_dom2	3.58e-38	416	595	6	182	Beta-galactosidase, domain 2. This is the second domain of the five-domain beta-galactosidase enzyme that altogether catalyses the hydrolysis of beta(1-3) and beta(1-4) galactosyl bonds in oligosaccharides as well as the inverse reaction of enzymatic condensation and trans-glycosylation. This domain is made up of 16 antiparallel beta-strands and an alpha-helix at its C terminus. The fold of this domain appears to be unique. In addition, the last seven strands of the domain form a subdomain with an immunoglobulin-like (I-type Ig) fold in which the first strand is divided between the two beta-sheets. In penicillin spp this strand is interrupted by a 12-residue insertion which forms an additional edge-strand to the second beta-sheet of the sub-domain. The remainder of the second domain forms a series of beta-hairpins at its N terminus, four strands of which are contiguous with part of the Ig-like sub-domain, forming in total a seven-stranded antiparallel beta-sheet. This domain is associated with family Glyco_hydro_35, which is N-terminal to it, but itself has no metazoan members.
404274	BetaGal_dom4_5	9.10e-36	891	1003	1	111	Beta-galactosidase jelly roll domain. This domain is found in beta galactosidase enzymes. It has a jelly roll fold.
404274	BetaGal_dom4_5	1.49e-23	719	836	5	111	Beta-galactosidase jelly roll domain. This domain is found in beta galactosidase enzymes. It has a jelly roll fold.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QRW00634.1|GH35	0.0	48	1038	14	1007
QRV86584.1|GH35	0.0	53	1038	27	1007
QRW27580.1|GH35	0.0	52	1038	24	1005
QRW15140.1|GH35	0.0	53	1038	27	1007
QRW10069.1|GH35	0.0	54	1021	55	1072

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1TG7_A	5.12e-197	63	1034	2	966	Native structure of beta-galactosidase from Penicillium sp. [Penicillium sp.],1XC6_A Native Structure Of Beta-Galactosidase from Penicillium sp. in complex with Galactose [Penicillium sp.]
5IFP_A	4.10e-194	57	1032	35	1000	Structure Of Beta-galactosidase From Aspergillus Niger [Aspergillus niger CBS 513.88]
5IFT_A	1.14e-193	57	1032	35	1000	STRUCTURE OF E298Q-BETA-GALACTOSIDASE FROM ASPERGILLUS NIGER IN COMPLEX WITH 3-b-Galactopyranosyl glucose [Aspergillus niger CBS 513.88],5IHR_A Structure Of E298q-beta-galactosidase From Aspergillus Niger In Complex With Allolactose [Aspergillus niger CBS 513.88],5JUV_A STRUCTURE OF E298Q-BETA-GALACTOSIDASE FROM ASPERGILLUS NIGER IN COMPLEX WITH 6-b-Galactopyranosyl galactose [Aspergillus niger CBS 513.88],5MGC_A STRUCTURE OF E298Q-BETA-GALACTOSIDASE FROM ASPERGILLUS NIGER IN COMPLEX WITH 4-Galactosyl-lactose [Aspergillus niger CBS 513.88],5MGD_A STRUCTURE OF E298Q-BETA-GALACTOSIDASE FROM ASPERGILLUS NIGER IN COMPLEX WITH 6-Galactosyl-lactose [Aspergillus niger CBS 513.88]
4IUG_A	3.57e-193	56	1026	34	991	Crystal structure of beta-galactosidase from Aspergillus oryzae in complex with galactose [Aspergillus oryzae]
3OG2_A	1.58e-186	63	1027	22	990	Chain A, Beta-galactosidase [Trichoderma reesei],3OGR_A Chain A, Beta-galactosidase [Trichoderma reesei],3OGS_A Chain A, Beta-galactosidase [Trichoderma reesei],3OGV_A Chain A, Beta-galactosidase [Trichoderma reesei]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|A1D1Z9|BGALA_NEOFI	8.51e-198	57	1012	35	978	Probable beta-galactosidase A OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=lacA PE=3 SV=1
sp|A2QL84|BGALC_ASPNC	6.60e-197	59	1021	20	964	Probable beta-galactosidase C OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=lacC PE=3 SV=1
sp|Q700S9|BGALA_PENSQ	1.52e-196	57	1034	35	1006	Beta-galactosidase A OS=Penicillium sp. OX=5081 GN=lacA PE=1 SV=1
sp|Q4WS33|BGALA_ASPFU	1.58e-194	57	1012	35	978	Probable beta-galactosidase A OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=lacA PE=3 SV=2
sp|A1D199|BGALB_NEOFI	4.04e-194	58	1033	31	1008	Probable beta-galactosidase B OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=lacB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.544492	0.455495

TMHMM  Annotations     

Start	End
13	35