CAZyme Information: KAG6582970.1

Basic Information

• Species: Phytophthora cinnamomi
• Lineage: Oomycota; NA; ; Peronosporaceae; Phytophthora; Phytophthora cinnamomi
• CAZyme ID: KAG6582970.1
• CAZy Family: AA6
• CAZyme Description: putative endo-1,3-beta-glucanase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1103	JAFJYM010000105|CGC1	120412.22	5.0276

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_PcinnamomiGKB4	19981	N/A	0	19981

• Gene Location: location=JAFJYM010000105:701070-704456(-)

Full Sequence      

MTTARGLSTLLLLLWSCARCAWAVPGAELTLQLPFPVASHPLQLSSSLFAASERLPLDAR
VANTSHTAQPKLLADVPFPTGAWWTNLVLAEGQSPVASLPYVHRVLDEKLHVSFPFRVVA
PREIVEGFISQMVVSSRAAQATAATAATPVAHHVVNFDAFSTTLRFSRGQTEEFRVYLVR
GSPYVTMEYAHSRPVLEAMDGLQITRFKKLEGLTLMSGQAAPFATFAAELNNGQTWYLYA
SDAQLKLRLDANGQVTSDEPFTGVLRVALCLNAKVMPYLLESAPVYAVGGDVAHSVHPDD
ADKALLQFRWKTKSFSTFHGEAEQVSEKENNKLLMVALPHHMDVMQVQKSGQVLEKGKNK
VLSDLRYTSIRGLMEGVYGAVWHMTETLPSVEWNYADDGLFSDDFSGTLEDGNEFRADDQ
QLRLDMREKAKQAILKQLPLDVDTYPLPLSPDSYNFGKQVGRDARLLLIADKFSQEEVKQ
KLLAKVEMEIVEWLEATNANHFVYDQTFGGVITNDGWHDKEADYGNGYYNDHHFHYGYFI
YALAAIRKFDPAFIEKHAQACALLMGDIGTPLLDSNTTFFNDLPVRLLFPTARHKDWFVG
HSYASGLFPMEVGKSQESSSEDLNAYYALALFSSLDEKATEGQGDSSYHQYARLLLATEL
RSVKKYWHMAENSKIYEPVFSKNAMVGVVGETNVVYNTWFGDRAVYIHGINMLPFTPFTP
HLLDEEYVAREYALLSQDLPDLGQYDIWRSIVVMDHAILNAADAWDELNNTVKAFDTWSS
RTNAMHWIATRPSWFAQKNRPALSNPDLDADDKCFGFPACASAGENGTALMCCGTLPGCC
PSNLGCCPQEDPALLPSNACFGEHECAVLGLGCCNTIDGCCEPDPISGTVLGCCKNQHPV
ISNTTHKAVHAAKDSAVCYGEPLCAAAKLDCCGAPGGCCAGSGVKLDCCSSEATVSGSSS
PSHSSNANTCQGQPKCGAAGLDCCGWDGGCCKPDPVTGAKLDCCQDEASDEPSTEAIPIR
NGNGHHEDHDSDDSAAGEGLGDTVTRIFIGLGGAVLFIAVVYAVGLCYRRRGYASIDGDM
RAIYCAGMMVGVIGFFIFLIVTS

Enzyme Prediction     

No EC number prediction in KAG6582970.1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH81	74	787	4.3e-154	0.9742765273311897

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
407548	Glyco_hydro81C	3.37e-77	428	782	4	346	Glycosyl hydrolase family 81 C-terminal domain. Family of eukaryotic beta-1,3-glucanases. Within the Aspergillus fumigatus protein, two perfectly conserved Glu residues (E550 or E554) have been proposed as putative nucleophiles of the active site of the Engl1 endoglucanase, while the proton donor would be D475. The endo-beta-1,3-glucanase activity is essential for efficient spore release. This entry represents the helical C-terminal domain.
227785	Acf2	3.68e-75	77	788	89	748	Endoglucanase Acf2 [Carbohydrate transport and metabolism].
397619	Glyco_hydro_81	2.02e-48	75	396	12	317	Glycosyl hydrolase family 81. Family of eukaryotic beta-1,3-glucanases. Within the Aspergillus fumigatus protein ENGL1, two perfectly conserved Glu residues (E550 or E554) have been proposed as putative nucleophiles of the active site of the Engl1 endoglucanase, while the proton donor would be D475. The endo-beta-1,3-glucanase activity is essential for efficient spore release.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
UIZ29064.1|GH81	0.0	11	1103	8	1080
CCA18874.1|GH81	1.40e-232	63	906	12	855
AGP40408.1|GH81	1.18e-105	64	792	63	702
AUX36386.1|GH81	1.12e-104	77	792	90	713
CAN97272.1|GH81	3.41e-104	72	792	88	718

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4K3A_A	6.23e-49	79	804	42	731	The structure of a glycoside hydrolase family 81 endo-[beta]-1,3-glucanase [Rhizomucor miehei],4K3A_B The structure of a glycoside hydrolase family 81 endo-[beta]-1,3-glucanase [Rhizomucor miehei]
5XBZ_A	7.88e-49	79	804	67	756	Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminaripentaose [Rhizomucor miehei],5XBZ_B Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminaripentaose [Rhizomucor miehei],5XC2_A Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminarihexaose [Rhizomucor miehei],5XC2_B Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminarihexaose [Rhizomucor miehei],5XC2_C Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminarihexaose [Rhizomucor miehei],5XC2_D Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminarihexaose [Rhizomucor miehei]
4K35_A	1.51e-46	79	804	42	731	The structure of a glycoside hydrolase family 81 endo-[beta]-1,3-glucanase [Rhizomucor miehei],4K35_B The structure of a glycoside hydrolase family 81 endo-[beta]-1,3-glucanase [Rhizomucor miehei]
5V1W_A	1.00e-29	77	788	48	689	Chain A, Glycoside Hydrolase [Halalkalibacterium halodurans C-125]
5T4A_A	1.11e-29	77	788	68	709	Chain A, Glycoside Hydrolase [Halalkalibacterium halodurans C-125],5T4G_A Chain A, Glycoside Hydrolase [Halalkalibacterium halodurans C-125]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q09850|ENG2_SCHPO	1.85e-40	178	766	145	674	Ascus wall endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng2 PE=1 SV=1
sp|D4AZ24|ENG1_ARTBC	1.80e-38	164	765	328	873	Probable endo-1,3(4)-beta-glucanase ARB_01444 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_01444 PE=1 SV=1
sp|Q5AIR7|ENG1_CANAL	1.26e-35	154	789	550	1139	Endo-1,3(4)-beta-glucanase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=ENG1 PE=1 SV=1
sp|P53753|ENG1_YEAST	4.36e-34	75	785	431	1106	Endo-1,3(4)-beta-glucanase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DSE4 PE=1 SV=1
sp|Q9UT45|ENG1_SCHPO	4.99e-34	454	771	413	714	Primary septum endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng1 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000232	0.999749	CS pos: 23-24. Pr: 0.9733

TMHMM  Annotations     

Start	End
1047	1069
1082	1101