CAZyme Information: KAG5438354.1

Basic Information

• Species: Pneumocystis canis
• Lineage: Ascomycota; Pneumocystidomycetes; ; Pneumocystidaceae; Pneumocystis; Pneumocystis canis
• CAZyme ID: KAG5438354.1
• CAZy Family: GT20
• CAZyme Description: unspecified product

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1476		172973.04	6.1025

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_PcanisCanA	3113	N/A	38	3075

• Gene Location: location=JACEFK010000008:192874-198112(+)

Full Sequence      

MYFFAAQNEALFFPLLDAISKDKNVILTKINLNNYNYLYKMIYEHEILTDEISLSILDYA
LALQSSAPKVQAYYHYYNDTVVSEMIHKNFNLHCKNWILFNEKQYCDINEFLKSKDIKEK
NVKPITLLPFDHVFHNYSIGDSPTVVLYADLSSYDYVIYHSFLYEEAKLGNLKYVFRYKP
EASDSKNLLYLSGYGVEASLKKTDYLVMDDREIIEDFNENTFFENLIPDSDSDPFLSSIQ
NDEIKPLTNAELKELGLSVTQFVMKSQNPLGTLTQIVQDLPKYASYLSTVKIGKENFEKL
IFNHQYTDPGKTMLFINGHQIDHFKINGFSLLKHLREEYNLIMSLQKLGLSAKKAKSLLT
HKNIFDLFDKFFNIRFDFRDNYEGSNVILWLNDIENDSNYIHFSKNIYEYFDDLPPGQLH
MIRYNLHHVIIPLDFSKKIDIFFFKSVFFFIQGFFPIRFGIVPVWHNPDDILLVKIFYFL
FNTYNFDFAFEYILYSDFGSLRDKSILLKKLQYILLKHGLYAELDKLDVNDIVESEEIEQ
YILKTKKWLLRLNVENGDFLIVNGKFIEKKLNWMNSLFDVLINDISEKKPANNVNIIEYL
LEDAIFMRDSYIHPINRTIKCQKLTDFMFDQRNIQFSSINENLNTELSLWLIADFDTREG
LKFAESSLKYMIKHPHTSLRFLHNPSLSEKKSEFSIFLFLLNESDIHVARSVLNYIQELL
DSYDSGNNINVRVKYPDFILNTYNSEQLRLSSIKAKSYWGKMRDSLNKLGFSPGQFGLLA
NGYIIGPLFEPYDFSLDNFQSLGSFFNFSIIFQLKAITYSLDIKNSKVKMFFPVLSSIIL
SDMISEKHKFENYPWVGKNNIYTLFQNLIPSFVVGNETDCLFEVKAIIDPLSEISQKFVS
ILNVLKQMKGVYLKIYMNPLLEISELSINRFYRYVLHSSLKFDNDGELIYPNAIFEKLPK
SHLYTIDYDFPGSWIVTQKTSIYDLDNLLLSDLSSKKITRIKVVYELKYILIEGHAEDIS
LKEIPAGVQLALKTKNNNFVTDTIIMNNFGYFQFKANPGIFKIDIINGESSNVFEISDIF
HRFESDSNKFTEGIILESFEGLTIFPKLIRKPGKENYSILKNVNSNKNDNSEFGSTASKA
TQFEKVNEFTFETSMQKSHAEINIFSIASGHLYERFLYIMILSVLKHTKHTVKVWFIENF
LSSSFKNSLPYVADEFGFQYELITYRWPHWLHSQEEKQRQIWGYKILFLDVLFPLDLDNI
IFVDADQIVRTDLKELVDMDLQGAPYGYTPMCDSRKEMEDYRFWKKGYWKSHLRGKPYHI
SALYVVDLKKFREIAAGDILRQHYQTLSRDPGSLSNLDQDLPNNLQHLIPIFSLSQNWLW
CETWCSDESLKDAKTIDLCNNPMTKESKLKRARRQVQEWNEYDKTVTDLIKRILEQGKSN
EINEHSLDGIVTPVNTIQNMQSNFDEKINKDIHNEL

Enzyme Prediction     

EC	2.4.1.-:29

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT24	1162	1409	1.1e-129	0.9959677419354839

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
408203	Glyco_transf_24	0.0	1162	1429	1	268	Glucosyltransferase 24. This is the catalytic domain found in UDP-glucose:glycoprotein glucosyltransferase (UGGT). This domain belongs to glucosyltransferase 24 family (GT24) A-type domain. The GT domain displays the expected glycosyltransferase type A (GT-A) fold.
133054	GT8_HUGT1_C_like	7.55e-169	1162	1409	1	248	The C-terminal domain of HUGT1-like is highly homologous to the GT 8 family. C-terminal domain of glycoprotein glucosyltransferase (UGT). UGT is a large glycoprotein whose C-terminus contains the catalytic activity. This catalytic C-terminal domain is highly homologous to Glycosyltransferase Family 8 (GT 8) and contains the DXD motif that coordinates donor sugar binding, characteristic for Family 8 glycosyltransferases. GT 8 proteins are retaining enzymes based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. The non-catalytic N-terminal portion of the human UTG1 (HUGT1) has been shown to monitor the protein folding status and activate its glucosyltransferase activity.
132996	Glyco_transf_8	1.12e-56	1162	1402	1	241	Members of glycosyltransferase family 8 (GT-8) are involved in lipopolysaccharide biosynthesis and glycogen synthesis. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. GT-8 comprises enzymes with a number of known activities: lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, glycogenin glucosyltransferase, and N-acetylglucosaminyltransferase. GT-8 enzymes contains a conserved DXD motif which is essential in the coordination of a catalytic divalent cation, most commonly Mn2+.
408201	Thioredoxin_14	8.88e-44	376	612	1	248	Thioredoxin-like domain. This is the third out of four TRXL(thioredoxin-like) domains found in UDP-glucose:glycoprotein glucosyltransferase (UGGT).
399437	UDP-g_GGTase	7.07e-42	1013	1120	1	108	UDP-glucose:Glycoprotein Glucosyltransferase. UDP-g_GGTase is an important, central component of the QC system in the ER for checking that glycoproteins are folded correctly. This QC prevents incorrectly folded glycoproteins from leaving the ER.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QSL64450.1|GT24	0.0	5	1476	53	1355
QSS69256.1|GT24	1.27e-293	5	1436	56	1469
QSS63494.1|GT24	3.44e-293	5	1436	67	1480
QSS51253.1|GT24	4.83e-293	5	1438	56	1471
QDS71787.1|GT24	1.83e-288	5	1438	53	1471

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5NV4_A	4.06e-268	5	1436	33	1454	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
5MU1_A	3.13e-267	5	1436	33	1454	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5MZO_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TRF_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TRT_A	3.39e-266	5	1436	33	1454	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TS8_A	8.04e-231	5	1371	26	1382	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS8_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TS2_A	6.44e-220	5	1436	33	1220	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_C Chain C, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_D Chain D, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q09140|UGGG_SCHPO	3.16e-235	47	1436	85	1431	UDP-glucose:glycoprotein glucosyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=gpt1 PE=1 SV=2
sp|Q9NYU1|UGGG2_HUMAN	3.62e-194	3	1445	55	1513	UDP-glucose:glycoprotein glucosyltransferase 2 OS=Homo sapiens OX=9606 GN=UGGT2 PE=1 SV=4
sp|Q6P5E4|UGGG1_MOUSE	3.21e-187	55	1429	116	1523	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Mus musculus OX=10090 GN=Uggt1 PE=1 SV=4
sp|Q9NYU2|UGGG1_HUMAN	9.61e-184	3	1429	67	1523	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Homo sapiens OX=9606 GN=UGGT1 PE=1 SV=3
sp|Q9JLA3|UGGG1_RAT	3.32e-182	55	1429	116	1523	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Rattus norvegicus OX=10116 GN=Uggt1 PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000052	0.000001

TMHMM  Annotations     

There is no transmembrane helices in KAG5438354.1.