CAZyme Information: KAG1712040.1

Basic Information

• Species: Phytophthora capsici
• Lineage: Oomycota; NA; ; Peronosporaceae; Phytophthora; Phytophthora capsici
• CAZyme ID: KAG1712040.1
• CAZy Family: GT71
• CAZyme Description: unspecified product

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1217		135537.36	5.0499

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_PcapsiciLT1534	28579	763924	5206	23373

• Gene Location: location=JADEVP010000002:2316192-2319845(-)

Full Sequence      

MLPRQDSGDGHAAVQRAPQSSSVVALKYVNTFFLALCFALLFVSCGAEQPEIQAQNSVDS
KSTALSHFNGLHTLSGASYRAFKNRLKRQMRGESCLRYHVAGTWWHYEWCFDHHVRQFHP
LPKDQEQSILLGVFNSKKPQPLRVVEVDNLNRLTDPDRMGYMAQQRYNSGDFCEARGTQR
SVSLQVKCCVFHDNETYVESVEEKAPCEYEMNVCSPVACGLMQRDQFVLTAPTSMEEEER
KALTKTVRDMFYHAYNGYLTHAFPQDDLLPLSCKGGEFELGRLPMLTLIDTLDTLALLED
ATEFQRAVGLVVENADFDLDTEVSVFETTIRLLGGLLSAHLFAVNKELKLFPENGYDGAL
LRLATDLGDRLMPAFDTVTGIPYGTVNLKYGVPKGETPIASTAGAGSLSIEFTMLSVLTG
EPRYAAASRGAVRALFQRRSKIGLLGKHINTKTGDWTETSSGPGSNSDSFYEYLLKMYEL
FGDRESLEMFAQVYPAVLAHNKHGDWYTDVSMYSGCHHHSGSSAVVFESLASFWPGMQVA
TGDLTYAAQSMNAFYRVWRDYGFLSEQFNVGDWKPVKSRGGGGARYPLRPELIESTFYMH
EATNDSSWLRAGAHVVHSLQKYAKTPCGYASIADVESKKQEDYMPSFFLSETCKYLYLLF
NTTHFFRQGNYVMTTEAHPLPILPRKIVEPILQSTDAYVTSEVEYDNQFSPDRVMRCEAP
KFYDFFGYSVHYEGKVVARTSRCTPPAPSPAAILKASKAAAEKLAKTTEEDSVMPTIEEI
AKAASDNAKPTTTEGDKASSERVASDVLQEWLPALEEKLRKMGKKNIDDNLLEQLLTESS
FGSTNHQPPVEHDAEGHVVQYVYGGSQLGEFRIEQLPGRLRVTREETGDWLEASGVLDTN
HMVIGLGLNDGSDDDAGADSYTFDEPTSAKVEQEDDYLPPHLSVNTVYKVNEDLTLPVDQ
RCSLRVQVGYKPYPGELQHNAKGKPHIDGDVKGKPNVWLTVPCVGAGFGVTDTFQASRAF
PEMELIVADPFDGCNEVSNLTEERVRGKIILVQRGECYFETKARNAAKWGAAGVIVVNTE
DDDLVMVMGGMEEGSENVTDGPLELPVVMIPQRLGEWFETRLAESEASSLSPVKVSIELT
VRHHDGNAFNNRRRARGIVDGSFPRVDGTADNMKVYGPLWGMELISVSSDRETRQTDEQH
HQESFTIAIVGTPPWQH

Enzyme Prediction     

EC	3.2.1.113:20

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH47	250	682	5e-133	0.9977578475336323

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
396217	Glyco_hydro_47	1.59e-135	250	682	1	453	Glycosyl hydrolase family 47. Members of this family are alpha-mannosidases that catalyze the hydrolysis of the terminal 1,2-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man(9)(GlcNAc)(2).
240427	PTZ00470	1.76e-81	221	684	54	520	glycoside hydrolase family 47 protein; Provisional
240122	PA_subtilisin_1	8.04e-19	1004	1131	1	117	PA_subtilisin_1: Protease-associated domain containing subtilisin-like proteases, subgroup 1. A subgroup of PA domain-containing subtilisin-like proteases. The significance of the PA domain to many of the proteins in which it is inserted is undetermined. It may be a protein-protein interaction domain. At peptidase active sites, the PA domain may participate in substrate binding and/or promoting conformational changes, which influence the stability and accessibility of the site to substrate. Proteins into which the PA domain is inserted include the following subtilisin-like proteases: i) melon cucumisin, ii) Arabidopsis thaliana Ara12, iii) Alnus glutinosa ag12, iv) members of the tomato P69 family, and v) tomato LeSBT2. However, these proteins belong to other subtilisin-like subgroups. Relatively little is known about proteins in this subgroup.
239047	PA_GO-like	4.17e-17	1004	1139	22	139	PA_GO-like: Protease-associated domain containing proteins like Arabidopsis thaliana growth-on protein GRO10. This group contains various PA domain-containing proteins similar to the functionally uncharacterized Arabidopsis GRO10. The PA domain may be a protein-protein interaction domain. At peptidase active sites, the PA domain may participate in substrate binding and/or promoting conformational changes, which influence the stability and accessibility of the site to substrate.
239038	PA_C_RZF_like	1.32e-16	1025	1116	44	131	PA_C-RZF_ like: Protease-associated (PA) domain C_RZF-like. This group includes various PA domain-containing proteins similar to C-RZF (chicken embryo RING zinc finger) protein. These proteins contain a C3H2C3 RING finger. C-RZF is expressed in embryo cells and is restricted mainly to brain and heart, it is localized to both the nucleus and endosomes. Additional C3H2C3 RING finger proteins belonging to this group, include Arabidopsis ReMembR-H2 protein and mouse sperizin. ReMembR-H2 is likely to be an integral membrane protein, and to traffic through the endosomal pathway. Sperizin is expressed in haploid germ cells and localized in the cytoplasm, it may participate in spermatogenesis. The significance of the PA domain to these proteins has not been ascertained. It may be a protein-protein interaction domain. At peptidase active sites, the PA domain may participate in substrate binding and/or promoting conformational changes, which influence the stability and accessibility of the site to substrate.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
UIZ27914.1|GH47	0.0	162	1214	1	1042
AIG56334.1|GH47	1.87e-243	86	1189	39	934
AIG55397.1|GH47	3.27e-234	95	1190	33	932
QCE07576.1|GH47	7.20e-127	238	685	30	471
BAT94804.1|GH47	9.76e-127	238	685	30	471

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4AYO_A	5.87e-53	238	689	8	441	Structure of The GH47 processing alpha-1,2-mannosidase from Caulobacter strain K31 [Caulobacter sp. K31],4AYP_A Structure of The GH47 processing alpha-1,2-mannosidase from Caulobacter strain K31 in complex with thiomannobioside [Caulobacter sp. K31],4AYQ_A Structure of The GH47 processing alpha-1,2-mannosidase from Caulobacter strain K31 in complex with mannoimidazole [Caulobacter sp. K31],4AYR_A Structure of The GH47 processing alpha-1,2-mannosidase from Caulobacter strain K31 in complex with noeuromycin [Caulobacter sp. K31],5MEH_A Crystal structure of alpha-1,2-mannosidase from Caulobacter K31 strain in complex with 1-deoxymannojirimycin [Caulobacter sp. K31],5NE5_A Crystal structure of family 47 alpha-1,2-mannosidase from Caulobacter K31 strain in complex with kifunensine [Caulobacter sp. K31]
5KIJ_A	4.60e-51	245	682	7	451	Crystal structure of the class I human endoplasmic reticulum 1,2-alpha-mannosidase and Man9GlcNAc2-PA complex [Homo sapiens]
1FMI_A	5.27e-51	245	682	12	456	Crystal Structure Of Human Class I Alpha1,2-Mannosidase [Homo sapiens]
1FO2_A	5.51e-51	245	682	12	456	Crystal Structure Of Human Class I Alpha1,2-Mannosidase In Complex With 1-Deoxymannojirimycin [Homo sapiens],1FO3_A Crystal Structure Of Human Class I Alpha1,2-Mannosidase In Complex With Kifunensine [Homo sapiens]
5KKB_A	9.09e-51	247	683	26	468	Structure of mouse Golgi alpha-1,2-mannosidase IA and Man9GlcNAc2-PA complex [Mus musculus],5KKB_B Structure of mouse Golgi alpha-1,2-mannosidase IA and Man9GlcNAc2-PA complex [Mus musculus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q9FG93|MNS4_ARATH	6.92e-125	234	685	31	476	Alpha-mannosidase I MNS4 OS=Arabidopsis thaliana OX=3702 GN=MNS4 PE=1 SV=1
sp|Q9SXC9|MNS5_ARATH	6.99e-117	219	683	13	479	Alpha-mannosidase I MNS5 OS=Arabidopsis thaliana OX=3702 GN=MNS5 PE=1 SV=1
sp|Q8BJT9|EDEM2_MOUSE	5.76e-112	230	680	28	480	ER degradation-enhancing alpha-mannosidase-like protein 2 OS=Mus musculus OX=10090 GN=Edem2 PE=1 SV=1
sp|Q9BV94|EDEM2_HUMAN	1.59e-111	247	680	39	480	ER degradation-enhancing alpha-mannosidase-like protein 2 OS=Homo sapiens OX=9606 GN=EDEM2 PE=1 SV=2
sp|Q925U4|EDEM1_MOUSE	1.03e-98	240	682	122	581	ER degradation-enhancing alpha-mannosidase-like protein 1 OS=Mus musculus OX=10090 GN=Edem1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.144679	0.855303	CS pos: 47-48. Pr: 0.4772

TMHMM  Annotations     

Start	End
21	43