CAZyme Information: KAF7631830.1

Basic Information

• Species: Aspergillus flavus
• Lineage: Ascomycota; Eurotiomycetes; ; Aspergillaceae; Aspergillus; Aspergillus flavus
• CAZyme ID: KAF7631830.1
• CAZy Family: GT62
• CAZyme Description: unspecified product

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
931	AAIH03000011|CGC9	104798.46	5.1771

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AflavusNRRL3357	14313	332952	0	14313

• Gene Location: location=AAIH03000011:1476125-1479141(+)

Full Sequence      

MRFTATAAALVASSIPATLGQHVRDLSNEKWTLSSDALNHTVPGNLPSHAHLDLLKAGVI
DDPYHGLNDFNLRWIPESNWTYTTDKIKDLLKDAESTWLVFDGLDTFATVEFCGKYVAST
NNQYRQYSFDVSQILEGCNEDPILKIDFGSAPNIVNAIAEDRNSPVWPDGIQQTYEYPNR
WFMRKEQSDFGWDWGPAFAPAGPWKPAYIVQLPKAQNIHVLNTDLDIYRKGQINHLPPDQ
SQPWVVNASIDFVGSLPPNPSMSIEFKDTKSGEILTSKRIGNVTVSGNSVTGVTVLGGVT
PKLWWPLGLGDQNLYNITVTVTGHQNQTLAHVTKRTGFRTIFLNQRNITDAQLAQGIAPG
ANWHFEVNGHEFYAKGSNIIPPDAFWPRVTEARMARLFDAVVAGNQNMLRVWSSGIYLHD
FIYDLADERGILLWSEFEFSDALYPVDDAFLDNIAAEVVYNVRRVNHHPSLALWAGGNEI
ESLMLPTVERKAPEEYAKYVGEYEKLYISLILPLVYQNTRSITYSPSSTTEGYLDVDLSA
PVPMVERYHNTTPGSYYGDTDFYNYDSSVSFNSHVYPVGRFANEFGYHSMPSLQTWQQAV
DPEDLHFNSTTVMLRNHHYPAGGTFTDNFHNTSLGMGEMTIAVQRYYPIPNKLDSVANFS
AWCHATQLFQADMYKSEIQFYRRGSGMPERQLGSLYWQLEDIWQAPSWAGIEYGGRWKVL
HYVSRDIYQRIIVSPFWNYTTGDLDLYVTSDLWESAKGKVNLTWLDLSGTPLPHNAGTPG
SVPFNVGALNTTKIYSTNIKNLTLPNPKDAILVLSLSGEGHLPNSDKKTTFTHQNHFTPV
FPKDLALVDPGLELSYNTKSKTFTVEAKSGVSLYTWLDYPADVVGYFDENAFVLLPGQKK
EIGFTVQEDNTDGKWVQGVTVQSLWNQTLEK

Enzyme Prediction     

EC	3.2.1.25:4

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	28	764	2.4e-76	0.6462765957446809

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
225789	LacZ	2.72e-57	12	725	1	627	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
407625	Ig_mannosidase	2.39e-19	849	926	1	78	Ig-fold domain. This Ig-like fold domain is found in mannosidase enzymes.
407658	Mannosidase_ig	3.29e-10	742	844	1	91	Mannosidase Ig/CBM-like domain. This domain corresponds to domain 4 in the structure of Bacteroides thetaiotaomicron beta-mannosidase, BtMan2A. This domain has an Ig-like fold.
395572	Glyco_hydro_2	1.50e-06	239	339	13	106	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
236673	ebgA	1.28e-05	204	479	192	449	cryptic beta-D-galactosidase subunit alpha; Reviewed

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QMW39414.1|GH2	0.0	1	930	1	930
QMW27343.1|GH2	0.0	1	930	1	930
BAE57065.1|GH2	0.0	1	931	1	914
QRD81719.1|GH2	0.0	1	915	1	879
UDD55193.1|GH2	0.0	183	931	1	749

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4CVU_A	0.0	23	928	30	940	Structure of Fungal beta-mannosidase from Glycoside Hydrolase Family 2 of Trichoderma harzianum [Trichoderma harzianum],4UOJ_A Chain A, BETA-MANNOSIDASE GH2 [Trichoderma harzianum],4UOJ_B Chain B, BETA-MANNOSIDASE GH2 [Trichoderma harzianum]
6DDT_A	8.00e-120	31	924	18	864	mouse beta-mannosidase (MANBA) [Mus musculus],6DDU_A mouse beta-mannosidase bound to beta-D-mannose (MANBA) [Mus musculus]
2VJX_A	1.43e-81	31	905	15	817	Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]
2JE8_A	1.48e-81	31	905	17	819	Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JE8_B Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
7OP6_A	1.51e-81	31	905	17	819	Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP6_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_A Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q2UN00|MANBA_ASPOR	0.0	1	931	1	914	Beta-mannosidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=mndA PE=3 SV=1
sp|B8NP78|MANBA_ASPFN	0.0	1	931	1	914	Beta-mannosidase A OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=mndA PE=3 SV=1
sp|O74168|MANBA_ASPAC	0.0	17	926	20	932	Beta-mannosidase A OS=Aspergillus aculeatus OX=5053 GN=mndA PE=1 SV=1
sp|Q0CI48|MANBA_ASPTN	0.0	9	930	10	931	Beta-mannosidase A OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=mndA PE=3 SV=2
sp|Q5BCI8|MANBA_EMENI	0.0	9	927	10	924	Beta-mannosidase A OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=mndA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000361	0.999624	CS pos: 24-25. Pr: 0.4707

TMHMM  Annotations     

There is no transmembrane helices in KAF7631830.1.