CAZyme Information: KAF5687746.1

Basic Information

• Species: Fusarium circinatum
• Lineage: Ascomycota; Sordariomycetes; ; Nectriaceae; Fusarium; Fusarium circinatum
• CAZyme ID: KAF5687746.1
• CAZy Family: GT2
• CAZyme Description: alpha-L-fucosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
500		56124.64	6.0648

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_FcircinatumNRRL25331	13905	N/A	0	13905

• Gene Location: location=JAAQPE010000071:52355-53857(+)

Full Sequence      

MTLSTKLAVVAAALFPINAAAQASGPYEATWESTDKHNASPEWFRDAKFGVYWHWGAFTT
PQYASEWYPRNMYEPGSDQRKHHTATYGPPEEWGYANFINGADDLKGNFVQFKPVLSSKG
GEFDPEAIIKAVKASGAKFAGPVGEHHDGYSMWDRKVNEWNSVKRGPNLDLVKLWADLVR
KNGMKLVVAMHQAYNYNGFYEWAPKTNDTSLRKLLGQLSREESDQLWFDKHREMLDHVQP
DIIWNDFSLDSPGYCADFDGPCAVAEKKRLDFLAYYFNRAVEWKKEVVTTHKHFDVGFRD
TSTVSDYERGGPANITRPYWLTDDAISASSWSYTVGIKYYSSKAMVHSLLDRISKNGNML
LNISPTAVGVLPDEQLKVLQDIGDFLGRYGESVFSTRAWDIYGEGPNQVTGGSFTAPLQG
NSSDVRFTRNKDANILYATVLGWPEDQHVSISSLGSDALVDLKSLKSIKLLGDQAGEYEK
VSDWKQTKDAPRDFAASSTS

Enzyme Prediction     

EC	3.2.1.51:2

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH29	16	394	2.8e-85	0.9479768786127167

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
214829	Alpha_L_fucos	2.24e-119	21	454	1	384	Alpha-L-fucosidase. O-Glycosyl hydrolases (EC 3.2.1.-) are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of 85 different families. This classification is available on the CAZy (CArbohydrate-Active EnZymes) web site. Because the fold of proteins is better conserved than their sequences, some of the families can be grouped in 'clans'. Family 29 encompasses alpha-L-fucosidases, which is a lysosomal enzyme responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins. Deficiency of alpha-L-fucosidase results in the lysosomal storage disease fucosidosis.
395889	Alpha_L_fucos	1.17e-117	22	391	1	333	Alpha-L-fucosidase.
226195	AfuC	2.54e-51	54	448	1	374	Alpha-L-fucosidase [Carbohydrate transport and metabolism].
407025	Fucosidase_C	2.54e-05	424	489	8	65	Alpha-L-fucosidase C-terminal domain. The C-terminal domain of Structure 1hl8 is constructed of eight anti-parallel-strands packed into two-sheets of five and three strands, respectively, forming a two-layer-sandwich containing a Greek key motif.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CCT67811.1|GH29	0.0	1	490	1	493
QGI94762.1|GH29	0.0	1	490	1	493
APG32860.1|GH29	0.0	1	490	8	500
EWY82402.1|GH29	0.0	1	490	2	494
ENH64923.1|GH29	0.0	1	490	1	493

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4NI3_A	1.39e-305	21	490	1	470	Chain A, Alpha-fucosidase GH29 [Fusarium graminearum],4NI3_B Chain B, Alpha-fucosidase GH29 [Fusarium graminearum],4PSP_A Chain A, Alpha-fucosidase GH29 [Fusarium graminearum],4PSP_B Chain B, Alpha-fucosidase GH29 [Fusarium graminearum],4PSR_A Chain A, Alpha-fucosidase GH29 [Fusarium graminearum],4PSR_B Chain B, Alpha-fucosidase GH29 [Fusarium graminearum]
7LJJ_A	8.49e-79	27	487	43	486	Chain A, Exo-alpha-L-galactosidase [Phocaeicola plebeius DSM 17135],7LK7_A Chain A, Exo-alpha-L-galactosidase [Phocaeicola plebeius DSM 17135]
7LNP_A	4.58e-78	27	487	43	486	Chain A, Exo-alpha-L-galactosidase [Phocaeicola plebeius DSM 17135],7LNP_C Chain C, Exo-alpha-L-galactosidase [Phocaeicola plebeius DSM 17135],7LNP_D Chain D, Exo-alpha-L-galactosidase [Phocaeicola plebeius DSM 17135],7LNP_E Chain E, Exo-alpha-L-galactosidase [Phocaeicola plebeius DSM 17135]
1HL8_A	4.31e-42	27	458	8	405	Crystal Structure Of Thermotoga Maritima Alpha-Fucosidase [Thermotoga maritima MSB8],1HL8_B Crystal Structure Of Thermotoga Maritima Alpha-Fucosidase [Thermotoga maritima MSB8],1HL9_A Crystal Structure Of Thermotoga Maritima Alpha-Fucosidase In Complex With A Mechanism Based Inhibitor [Thermotoga maritima MSB8],1HL9_B Crystal Structure Of Thermotoga Maritima Alpha-Fucosidase In Complex With A Mechanism Based Inhibitor [Thermotoga maritima MSB8]
1ODU_A	5.95e-42	27	458	8	405	Crystal Structure Of Thermotoga Maritima Alpha-Fucosidase In Complex With Fucose [Thermotoga maritima MSB8],1ODU_B Crystal Structure Of Thermotoga Maritima Alpha-Fucosidase In Complex With Fucose [Thermotoga maritima MSB8]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q99KR8|FUCO2_MOUSE	3.19e-31	27	444	28	397	Plasma alpha-L-fucosidase OS=Mus musculus OX=10090 GN=Fuca2 PE=1 SV=1
sp|P17164|FUCO_RAT	2.47e-29	14	453	16	408	Tissue alpha-L-fucosidase OS=Rattus norvegicus OX=10116 GN=Fuca1 PE=1 SV=1
sp|Q5RFI5|FUCO2_PONAB	2.56e-29	27	444	32	401	Plasma alpha-L-fucosidase OS=Pongo abelii OX=9601 GN=FUCA2 PE=2 SV=1
sp|C3YWU0|FUCO_BRAFL	5.33e-29	27	451	18	393	Alpha-L-fucosidase OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_56888 PE=3 SV=2
sp|Q9VTJ4|FUCO_DROME	6.44e-29	21	457	25	421	Putative alpha-L-fucosidase OS=Drosophila melanogaster OX=7227 GN=Fuca PE=2 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000286	0.999688	CS pos: 21-22. Pr: 0.9769

TMHMM  Annotations     

There is no transmembrane helices in KAF5687746.1.