CAZyme Information: KAF5685470.1

Basic Information

• Species: Fusarium circinatum
• Lineage: Ascomycota; Sordariomycetes; ; Nectriaceae; Fusarium; Fusarium circinatum
• CAZyme ID: KAF5685470.1
• CAZy Family: GH7
• CAZyme Description: xyloglucan endo-transglycosylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
267	JAAQPE010000110|CGC1	29883.85	5.1745

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_FcircinatumNRRL25331	13905	N/A	0	13905

• Gene Location: location=JAAQPE010000110:4623-5551(+)

Full Sequence      

MAFSDRLETKFSQLHNISEGHDWVAQNFTVSAADGRGNFGKAFLPNNVNTHETKSGDDSG
LELRVRSAVNKDDAIPSSEVDTARLDLHWGSFRAGMKLTDYFNDTQEIDIEFLSQEFDHD
NGIYPINLVVQSKKSLEAGYDASKTGSFKRVKLDFDPTDAFHEYRFDYLPGQVLFYADGK
LLAHMKGADMPSSGGHLILQHWSNGNSLWSGGPPKEDATVTVSYVKAYFNSSNSERQSQL
ARRCHESSEETSVLAQRESRQEPLSVF

Enzyme Prediction     

No EC number prediction in KAF5685470.1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH16	51	228	1.6e-77	0.96875

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
185683	Glyco_hydrolase_16	3.69e-38	22	228	12	210	glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.
185684	GH16_lichenase	2.69e-09	32	224	15	205	lichenase, member of glycosyl hydrolase family 16. Lichenase, also known as 1,3-1,4-beta-glucanase, is a member of glycosyl hydrolase family 16, that specifically cleaves 1,4-beta-D-glucosidic bonds in mixed-linked beta glucans that also contain 1,3-beta-D-glucosidic linkages. Natural substrates of beta-glucanase are beta-glucans from grain endosperm cell walls or lichenan from the Islandic moss, Cetraria islandica. This protein is found not only in bacteria but also in anaerobic fungi. This domain includes two seven-stranded antiparallel beta-sheets that are adjacent to one another forming a compact, jellyroll beta-sandwich structure.
395585	Glyco_hydro_16	1.62e-06	107	226	64	168	Glycosyl hydrolases family 16.
185693	GH16_laminarinase_like	1.55e-04	107	228	115	235	Laminarinase, member of the glycosyl hydrolase family 16. Laminarinase, also known as glucan endo-1,3-beta-D-glucosidase, is a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CCT71404.1|GH16_23	2.67e-139	1	255	34	252
QGI84375.1|GH16_23	2.67e-139	1	255	34	252
QGI98026.1|GH16_23	2.67e-139	1	255	34	252
QGI67140.1|GH16_23	2.67e-139	1	255	34	252
QKD58555.1|GH16_23	8.42e-136	1	255	34	252

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1CPN_A	2.47e-08	107	236	47	174	NATIVE-LIKE IN VIVO FOLDING OF A CIRCULARLY PERMUTED JELLYROLL PROTEIN SHOWN BY CRYSTAL STRUCTURE ANALYSIS [Paenibacillus macerans]
1MAC_A	3.51e-08	107	211	103	192	Crystal Structure And Site-Directed Mutagenesis Of Bacillus Macerans Endo-1,3-1,4-Beta-Glucanase [Paenibacillus macerans],1MAC_B Crystal Structure And Site-Directed Mutagenesis Of Bacillus Macerans Endo-1,3-1,4-Beta-Glucanase [Paenibacillus macerans]
1AJK_A	3.59e-08	107	211	22	111	Circularly Permuted (1-3,1-4)-Beta-D-Glucan 4- Glucanohydrolase Cpa16m-84 [Paenibacillus macerans],1AJK_B Circularly Permuted (1-3,1-4)-Beta-D-Glucan 4- Glucanohydrolase Cpa16m-84 [Paenibacillus macerans]
1BYH_A	3.59e-08	107	211	105	194	MOLECULAR AND ACTIVE-SITE STRUCTURE OF A BACILLUS (1-3,1-4)-BETA-GLUCANASE [synthetic construct],1GLH_A Cation Binding To A Bacillus (1,3-1,4)-Beta-Glucanase. Geometry, Affinity And Effect On Protein Stability [Paenibacillus macerans],2AYH_A Crystal And Molecular Structure At 1.6 Angstroms Resolution Of The Hybrid Bacillus Endo-1,3-1,4-Beta-D-Glucan 4- Glucanohydrolase H(A16-M) [hybrid]
1CPM_A	3.59e-08	107	211	47	136	NATIVE-LIKE IN VIVO FOLDING OF A CIRCULARLY PERMUTED JELLYROLL PROTEIN SHOWN BY CRYSTAL STRUCTURE ANALYSIS [Paenibacillus macerans]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P23904|GUB_PAEMA	2.30e-07	107	211	128	217	Beta-glucanase OS=Paenibacillus macerans OX=44252 PE=1 SV=2
sp|P45797|GUB_PAEPO	1.42e-06	107	211	129	218	Beta-glucanase OS=Paenibacillus polymyxa OX=1406 GN=gluB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.999982	0.000046

TMHMM  Annotations     

There is no transmembrane helices in KAF5685470.1.