CAZyme Information: KAF5679075.1

Basic Information

• Species: Fusarium circinatum
• Lineage: Ascomycota; Sordariomycetes; ; Nectriaceae; Fusarium; Fusarium circinatum
• CAZyme ID: KAF5679075.1
• CAZy Family: GH3
• CAZyme Description: trehalose phosphate synthase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
625	JAAQPE010000208|CGC1	70226.52	5.3910

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_FcircinatumNRRL25331	13905	N/A	0	13905

• Gene Location: location=JAAQPE010000208:342866-345359(-)

Full Sequence      

MRSDQRHRFATQQSKAPYTNSTTGDLRLSTLNQLPGQFPAPPQSTIDHRPSTTNRDEADY
TPTFLFLKLSSFNFSTSQISLFEIISSNTVIMPGAVDENGQAAPGRLLLLSNRLPITIKR
SEDGSYSFSMSSGGLVTGLSGLSKTTSFQWYGWPGLEVPDNEVEGMKQRLKDEYGAHPVF
IDDELADRHYNGFSNSILWPLFHYHPGEITFDESAWAAYQEVNRLFAKTVIKDVQDGDLI
WVHDYHLMLLPQMLREEIGESKKNVKIGFFLHTPFPSSEIYRILPVREALLTGLLDCDLI
GFHTYDYARHFLSSCSRILECPTTPNGVDWNGRFVTVGAFPIGIDPDNFVEGLKKPKVQE
RIAALSRKFEGVKLVVGVDRLDYIKGVPQKLHALEVFLTEHPEWIGKIVLVQVAVPSRQD
VEEYQNLRAVVNELVGRINGKFGTIEFMPIHFLHQSVTFDELTALYAVSDVCLVSSTRDG
MNLVSYEYIATQQKNHGVMILSEFTGAAQSLNGSLIVNPWNTEELANALHDAVTMSPEQR
EANYKKLERYVFKYTSAWWGASFVSEMTRLSTEGSQPKTLRNVSGAVIGLEQKAQQAVAD
LEKKAEELLVIDEKKEADLQTEPSQ

Enzyme Prediction     

EC	2.4.1.15:146

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT20	103	568	3.9e-202	0.9747368421052631

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
215555	PLN03063	0.0	98	623	4	535	alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional
274112	trehalose_OtsA	0.0	106	569	1	456	alpha,alpha-trehalose-phosphate synthase [UDP-forming]. This enzyme catalyzes the key, penultimate step in biosynthesis of trehalose, a compatible solute made as an osmoprotectant in some species in all three domains of life. The gene symbol OtsA stands for osmotically regulated trehalose synthesis A. Trehalose helps protect against both osmotic and thermal stresses, and is made from two glucose subunits. This model excludes glucosylglycerol-phosphate synthase, an enzyme of an analogous osmoprotectant system in many cyanobacterial strains. This model does not identify archaeal examples, as they are more divergent than glucosylglycerol-phosphate synthase. Sequences that score in the gray zone between the trusted and noise cutoffs include a number of yeast multidomain proteins in which the N-terminal domain may be functionally equivalent to this family. The gray zone also includes the OtsA of Cornyebacterium glutamicum (and related species), shown to be responsible for synthesis of only trace amounts of trehalose while the majority is synthesized by the TreYZ pathway; the significance of OtsA in this species is unclear (see Wolf, et al., ). [Cellular processes, Adaptations to atypical conditions]
184712	PRK14501	0.0	105	612	1	498	putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional
395781	Glyco_transf_20	0.0	105	569	1	470	Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex,.
223457	OtsA	0.0	104	566	14	476	Trehalose-6-phosphate synthase [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AMD38858.1|GT20	0.0	92	625	1	534
AMD38875.1|GT20	0.0	92	625	1	534
AMD38873.1|GT20	0.0	92	625	1	534
AMD38874.1|GT20	0.0	92	625	1	534
AMD38862.1|GT20	0.0	92	625	1	534

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6JBI_A	4.25e-319	106	570	1	465	Structure of Tps1 apo structure [Pyricularia oryzae 70-15],6JBI_B Structure of Tps1 apo structure [Pyricularia oryzae 70-15],6JBR_A Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_B Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_D Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_F Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_H Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_K Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_M Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_O Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBW_A Structure of Tps1/UDP complex [Pyricularia oryzae 70-15],6JBW_B Structure of Tps1/UDP complex [Pyricularia oryzae 70-15]
5HVM_A	7.92e-292	98	575	5	482	Structure of Aspergillus fumigatus trehalose-6-phosphate synthase A in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293],5HVM_B Structure of Aspergillus fumigatus trehalose-6-phosphate synthase A in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293]
5HVO_A	1.99e-271	99	572	5	479	Structure of Aspergillus fumigatus trehalose-6-phosphate synthase B in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293],5HVO_B Structure of Aspergillus fumigatus trehalose-6-phosphate synthase B in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293],5HVO_C Structure of Aspergillus fumigatus trehalose-6-phosphate synthase B in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293],5HVO_D Structure of Aspergillus fumigatus trehalose-6-phosphate synthase B in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293]
5HUT_A	8.41e-249	105	575	4	475	Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP-glucose [Candida albicans SC5314],5HUT_B Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP-glucose [Candida albicans SC5314],5HUU_A Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP and glucose-6-phosphate [Candida albicans SC5314],5HUU_B Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP and glucose-6-phosphate [Candida albicans SC5314],5HVL_A Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP and validoxylamine A [Candida albicans SC5314],5HVL_B Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP and validoxylamine A [Candida albicans SC5314]
5HUV_A	3.41e-248	105	575	4	475	Structure of Candida albicans trehalose-6-phosphate synthase E341R/E346R in complex with UDP-glucose [Candida albicans SC5314],5HUV_B Structure of Candida albicans trehalose-6-phosphate synthase E341R/E346R in complex with UDP-glucose [Candida albicans SC5314]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|A0A384JAD8|TPS1_BOTFB	1.07e-309	92	596	1	504	Alpha,alpha-trehalose-phosphate synthase [UDP-forming] OS=Botryotinia fuckeliana (strain B05.10) OX=332648 GN=TPS1 PE=1 SV=1
sp|O59921|TPS1_EMENI	8.15e-293	92	595	1	504	Alpha,alpha-trehalose-phosphate synthase [UDP-forming] OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=tpsA PE=3 SV=1
sp|Q4WLM9|TPS1A_ASPFU	4.07e-291	98	575	5	482	Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 1 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=tpsA PE=1 SV=1
sp|Q00075|TPS1A_ASPNG	3.40e-288	106	576	14	484	Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 1 OS=Aspergillus niger OX=5061 GN=tpsA PE=1 SV=1
sp|Q4WHW0|TPS1B_ASPFU	1.03e-270	99	572	5	479	Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 2 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=tpsB PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.999824	0.000216

TMHMM  Annotations     

There is no transmembrane helices in KAF5679075.1.