CAZyme Information: KAF5664632.1

Basic Information

• Species: Fusarium circinatum
• Lineage: Ascomycota; Sordariomycetes; ; Nectriaceae; Fusarium; Fusarium circinatum
• CAZyme ID: KAF5664632.1
• CAZy Family: CBM63
• CAZyme Description: mannosyl-oligosaccharide alpha-1,2-mannosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
543	JAAQPE010000408|CGC1	60876.21	4.6517

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_FcircinatumNRRL25331	13905	N/A	0	13905

• Gene Location: location=JAAQPE010000408:128800-130431(-)

Full Sequence      

MMFFSLVLCTFVLSALASPSTPISEQKLGGLRYPKQRGKHYDYTGFEPSQKKRADDIIDM
FRFAWNGYYEHAFPHDSLRPKYSNYTDDRNGWGVTAVDGLDTAILMEQTDIVEIILDFIP
TINFTKTNTPEPTSVSLFETNIRYLGGLLSAYDLLKGPFKHLKVNKDNVDALLKQATTLA
DTLKFAFDTPTGIPFGSLWIENQTSTDEDSLEDGTAYAGLAAMGTLVLEWQHLSDLTGDP
QYGKLANRAESYWLSPSSEVWPGLTGGRFSLKTGEILDEYGGWTSGNDSAYEYLIKMYVY
DPDRFSHYGERFTLAADSTIKHLISHPSSRPDLTMAAAFTGKQQVNYTEFLACFIGGSFI
LGSSALDRPDWLDYGLSFSEYCANGYRYTASGIGPTLYSWDLNELTQPENKNQTLFYERA
GFFIQEGIFSGGQAPEAVESWYYAYQKTHDQYWRDVAWAYTLAQNRTMRVGGGFASISNV
LSPSGDGVRGGIMASFMLAETLKYMWLIQTENKGEWDVLEGGENLFVYNTEAHPLRVAAK
KPV

Enzyme Prediction     

EC	3.2.1.113:12

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH47	60	537	1.4e-109	0.9977578475336323

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
396217	Glyco_hydro_47	4.45e-141	60	537	1	453	Glycosyl hydrolase family 47. Members of this family are alpha-mannosidases that catalyze the hydrolysis of the terminal 1,2-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man(9)(GlcNAc)(2).
240427	PTZ00470	1.41e-74	17	540	30	521	glycoside hydrolase family 47 protein; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CCT73980.1|GH47	0.0	2	543	1	542
QGI69595.1|GH47	0.0	2	542	1	541
QGJ00483.1|GH47	0.0	2	542	1	541
QGI86948.1|GH47	0.0	2	542	1	541
QKD60002.1|GH47	0.0	2	543	1	542

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2RI8_A	4.45e-152	49	538	1	474	Penicillium citrinum alpha-1,2-mannosidase complex with glycerol [Penicillium citrinum],2RI8_B Penicillium citrinum alpha-1,2-mannosidase complex with glycerol [Penicillium citrinum],2RI9_A Penicillium citrinum alpha-1,2-mannosidase in complex with a substrate analog [Penicillium citrinum],2RI9_B Penicillium citrinum alpha-1,2-mannosidase in complex with a substrate analog [Penicillium citrinum]
1KKT_A	1.47e-151	49	538	36	509	Structure of P. citrinum alpha 1,2-mannosidase reveals the basis for differences in specificity of the ER and Golgi Class I enzymes [Penicillium citrinum],1KKT_B Structure of P. citrinum alpha 1,2-mannosidase reveals the basis for differences in specificity of the ER and Golgi Class I enzymes [Penicillium citrinum],1KRE_A Structure Of P. Citrinum Alpha 1,2-Mannosidase Reveals The Basis For Differences In Specificity Of The Er And Golgi Class I Enzymes [Penicillium citrinum],1KRE_B Structure Of P. Citrinum Alpha 1,2-Mannosidase Reveals The Basis For Differences In Specificity Of The Er And Golgi Class I Enzymes [Penicillium citrinum],1KRF_A Structure Of P. Citrinum Alpha 1,2-mannosidase Reveals The Basis For Differences In Specificity Of The Er And Golgi Class I Enzymes [Penicillium citrinum],1KRF_B Structure Of P. Citrinum Alpha 1,2-mannosidase Reveals The Basis For Differences In Specificity Of The Er And Golgi Class I Enzymes [Penicillium citrinum]
1HCU_A	4.80e-134	53	537	15	492	Chain A, ALPHA-1,2-MANNOSIDASE [Trichoderma reesei],1HCU_B Chain B, ALPHA-1,2-MANNOSIDASE [Trichoderma reesei],1HCU_C Chain C, ALPHA-1,2-MANNOSIDASE [Trichoderma reesei],1HCU_D Chain D, ALPHA-1,2-MANNOSIDASE [Trichoderma reesei]
5KKB_A	5.29e-39	50	537	20	467	Structure of mouse Golgi alpha-1,2-mannosidase IA and Man9GlcNAc2-PA complex [Mus musculus],5KKB_B Structure of mouse Golgi alpha-1,2-mannosidase IA and Man9GlcNAc2-PA complex [Mus musculus]
1NXC_A	6.11e-39	50	537	18	465	Structure of mouse Golgi alpha-1,2-mannosidase IA reveals the molecular basis for substrate specificity among Class I enzymes (family 47 glycosidases) [Mus musculus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P31723|MAN12_PENCI	7.55e-151	49	538	36	509	Mannosyl-oligosaccharide alpha-1,2-mannosidase OS=Penicillium citrinum OX=5077 GN=MSDC PE=1 SV=2
sp|A1CP08|MNS1B_ASPCL	1.72e-149	53	537	35	508	Probable mannosyl-oligosaccharide alpha-1,2-mannosidase 1B OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107) OX=344612 GN=mns1B PE=3 SV=1
sp|Q0D076|MNS1B_ASPTN	4.58e-146	16	537	3	507	Probable mannosyl-oligosaccharide alpha-1,2-mannosidase 1B OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=mns1B PE=3 SV=1
sp|Q12563|MNS1B_ASPPH	7.88e-143	51	539	40	512	Mannosyl-oligosaccharide alpha-1,2-mannosidase 1B OS=Aspergillus phoenicis OX=5063 GN=mns1B PE=2 SV=1
sp|Q2ULB2|MNS1B_ASPOR	1.43e-142	14	539	4	510	Mannosyl-oligosaccharide alpha-1,2-mannosidase 1B OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=mns1B PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000217	0.999753	CS pos: 17-18. Pr: 0.9793

TMHMM  Annotations     

There is no transmembrane helices in KAF5664632.1.