CAZyme Information: K441DRAFT_648823-t45_1-p1

Basic Information

• Species: Cenococcum geophilum
• Lineage: Ascomycota; Dothideomycetes; ; Gloniaceae; Cenococcum; Cenococcum geophilum
• CAZyme ID: K441DRAFT_648823-t45_1-p1
• CAZy Family: GH16
• CAZyme Description: glycoside hydrolase family 2 protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
927	KV748264|CGC2	103742.94	4.6049

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_Cgeophilum1.58	14745	794803	38	14707

• Gene Location: location=KV748264:193158-196404(+)

Full Sequence      

MARWNIQAFLTASTTSIACCQQKILDLSAIDWTVSNSNLSISVPGQLPSQAHLDLYAAHV
IGDPYYGLNDFNLRWIADSNWTYTSGPLIDLANSGSTWLLFNGLDTFTSIELCGKHVAST
NNQFRQYFFEVSSILNSCTVSPVLSINFGSAPQIANEIAAEPGQETWPTGVEGLFEFPNR
QFIRKEQSDFGWDWGPAFAPAGPWQPAYVIQLRAADIYVRNSLVDIYREGQLNNLPPDQT
KNWILNASLDCLGSLPSNAALNYRLLDKTNSTILSGPLANITSNNTTITGSVIIPDGAVD
LWWPNGMGSQTLYHLTIEVVDSSASTIASVNKRVGFRTIVLNEGVITQEQLDQGIAPGNN
WHFEINGHEFYAKGSNFIPPDAFWPTVTESRIRQLFDSVTAGNQNMLRVWASGAYSPDFM
YDLADEMGILLWSEFEFGDALYPVDPEFLENVREEVNYQVRRINHHPSLAYWAGGNELEN
LELSLVRAVAPDQLDRYTAEYEKLFLQTIVPVLFGNSRSISYSPSSTSNGYLALNFSWPI
PIVERYQNLTPGSLYGETDYYNYDSASAFDFGSYPVGRFANEFGYHSMPSLQSWQQAVAP
SDLHFNSSVIQLRNHHYPPGGLNTTNFSNTTKGMGEMTLAAQRWYPVPDKTDPIANFSAW
CHTTQIFQADFYSSQIQYYRRGSGMRERQLGSLYWQLEDIWQAPTWAGIEYDGRWKVLHY
VAKDIYQPVIISPFLDVTTHNFEVYVTSDLWSPATGSANFTWYDWSGNRLNISAPTFALV
TVGALNTTRVLQTNTYDVLSGYDPANVILVMDVSVKGQMPNSNTTQTFTHTNWFHTAPLS
QARLVDPGLSVSYSKETSKFTVKASKGIAAWVWLDYPAGAVLNFDSNTFWLLPGQEKEVG
FIVKNDTTGGKWVNGVTVRSLWDNMVP

Enzyme Prediction     

EC	3.2.1.25:4

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	37	762	2.7e-69	0.6223404255319149

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
225789	LacZ	8.24e-47	42	733	29	636	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
407625	Ig_mannosidase	1.07e-19	846	923	1	78	Ig-fold domain. This Ig-like fold domain is found in mannosidase enzymes.
407658	Mannosidase_ig	9.86e-10	741	817	2	75	Mannosidase Ig/CBM-like domain. This domain corresponds to domain 4 in the structure of Bacteroides thetaiotaomicron beta-mannosidase, BtMan2A. This domain has an Ig-like fold.
236673	ebgA	1.24e-08	216	477	213	449	cryptic beta-D-galactosidase subunit alpha; Reviewed
395572	Glyco_hydro_2	5.84e-05	238	337	13	106	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
SMR45057.1|GH2	0.0	27	927	23	928
SMY20222.1|GH2	0.0	23	927	22	931
SMQ46536.1|GH2	0.0	23	927	19	928
SMR42887.1|GH2	0.0	27	923	23	924
UJO11998.1|GH2	0.0	17	923	18	927

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4CVU_A	0.0	24	927	30	942	Structure of Fungal beta-mannosidase from Glycoside Hydrolase Family 2 of Trichoderma harzianum [Trichoderma harzianum],4UOJ_A Chain A, BETA-MANNOSIDASE GH2 [Trichoderma harzianum],4UOJ_B Chain B, BETA-MANNOSIDASE GH2 [Trichoderma harzianum]
6DDT_A	2.37e-113	32	923	18	866	mouse beta-mannosidase (MANBA) [Mus musculus],6DDU_A mouse beta-mannosidase bound to beta-D-mannose (MANBA) [Mus musculus]
2VJX_A	3.72e-82	32	749	15	672	Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]
2JE8_A	3.86e-82	32	749	17	674	Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JE8_B Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
7OP6_A	3.92e-82	32	749	17	674	Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP6_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_A Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q2UN00|MANBA_ASPOR	0.0	3	923	2	909	Beta-mannosidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=mndA PE=3 SV=1
sp|B8NP78|MANBA_ASPFN	0.0	3	923	2	909	Beta-mannosidase A OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=mndA PE=3 SV=1
sp|O74168|MANBA_ASPAC	0.0	22	923	24	932	Beta-mannosidase A OS=Aspergillus aculeatus OX=5053 GN=mndA PE=1 SV=1
sp|Q0CI48|MANBA_ASPTN	0.0	12	923	13	927	Beta-mannosidase A OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=mndA PE=3 SV=2
sp|Q5BCI8|MANBA_EMENI	0.0	25	923	24	923	Beta-mannosidase A OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=mndA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.985139	0.014880

TMHMM  Annotations     

There is no transmembrane helices in K441DRAFT_648823-t45_1-p1.