CAZyme Information: I317_07797-t43_1-p1

Basic Information

• Species: Kwoniella heveanensis
• Lineage: Basidiomycota; Tremellomycetes; ; Cryptococcaceae; Kwoniella; Kwoniella heveanensis
• CAZyme ID: I317_07797-t43_1-p1
• CAZy Family: GT90
• CAZyme Description: UDP-glucose:glycoprotein glucosyltransferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1679		186140.12	4.6873

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_KheveanensisCBS569	8002	1296119	131	7871

• Gene Location: location=KV700324:12436-19825(+)

Full Sequence      

MRVRGRGTAATASVVAALLSALLPFVAGANPGRGTDTPPVSISLETAWDAPPLILEILET
VYDESPSSYFPLLHLLSSLPSTDSTSPEDLLSSTLDLISSYNLLPGPSSLSTFNLALSLH
TAVPKIQAEYSWYEDTFRDVVSGGQEVKLGVVGCQGWVEWRGKGFCDVEEFRKDMEMSIE
DGQHSDAQPISLPFDHVSYSSSTNPRSPQAILYYTPSSPTASALLNYLGQHASAYPEFSY
IIRYLPPPQLSEGQRESAKKTPLNGWGVELALKKTDYLVVDDRATTSSSSSSSNDGEAQK
VMMADGNKNVSSVFRDVLGHDPWSDLATPLTNTEVKDLGLKAATLIMSSDDPLEALNHLS
QDFPKYSAALARQVEVPDDIRAKGRTIWQRGKGEAGIWINGKAMPELNGYSLLKTLRSER
DLVLSLTSLGLTPKQAIDLISDPVIGEAQVEDAPGEGVLDASDRIDGGDVIVYWNDIEKD
KSLRSLTPRSSQYMRQLYPGQFHTIRRNTWNLVFVLDLAQVPSLEIISSAISPMIQRGLP
IRFGIVPMFEPGKNDISAQMAKVFHYVVKSFGRGSTRDFLSALVSVTPSSHAAPGTVSLD
SVKKAYDLLASESTKAALPFEELLSSELFDAHLEKTESYMNRLLATKEESPVGHLFVNGK
HTVMGPHWTAVVQSEMASQLAHLQSQLMLGDQPENLDTYFYDLPSTATRRNRLINPGQGD
SKLRVFNLVSLFEGDVTKRLTEDFVYPGKLGTPITTWVVGDLDTDEGRRVVEDALKHLQT
PKCSLRLGFVHVPSSDSTTPRPGYKLSTALYQLQAQSLLSTITPGQLLELLGDIQENNEH
LAKCGAPSDAELEEGLSADSIIEDYDAHKVEQIINEDGSKQTCFTVKFKLTDEQKERYYE
GKPLQAATLGSTSVQNVAAAAEFWKIGSAIAEQLQLRDGKPHLLLNGRLVGPITRQNFIL
EDFDALEVYEHRKRVKPVIDLLKTMYDDISVFDRPTLAHLISMTSSVVAAAYKSAEAEGI
FTITQSSRDRYYKKLDDGLMSFTIGNPETAILEVAAVVDPISEQAQRWSTLLQTLAEMEN
VAVWVYLEPTPMLEEVKLKRFYRSSLPAQLTFDVDGDVIAPGVTFAHLPSTPIFTLGLDA
PSSWIVSPRTSPYDLDNLVLSHVHEPVHISFQLKQLLIEGHAREGTNAPPRGLQFQLTKN
GLEVASDTQVMANLGYLQFKATPGVYDLSIRPGRGTAVYDLESVGNEGWDSKSVNETGTG
VILDSFEGVTILPRFSRKLGMERADVLQEEEQAKQPESFAGAVFSKSVKLSAMFTRSGQG
YQSDIHADINIFTVASGLLYERFASIMILSVMKHTKSSVKFWFIENFLSPTFIEFIPRLA
EEYGFQYEFVTYKWPHWLRAQTEKQRIIWAYKILFLDVLFPMDLDKVIFVDADQIVRTDM
KELVDVDLHGRVYGYPPMGNSREEMEGFRFWKSGYWKEALRGRPYHISALYVVDLKRFRQ
LATGDRLRGQYHALSADPNSLANLDQDLPNSMQDTIPIWTLEQDWLWCQTWCSDESLATA
KTIDLCQNPLTKEPKLVRARQIPEWDTYDQEISAFAARLSDRQGGAGAGALAVSVDDLAS
EAGASVAKSGDQEQNKDEIADKGDIEDDQGLGISSETQTRDEGLVEELLEESRRIDDEL

Enzyme Prediction     

EC	2.4.1.-:29

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT24	1329	1576	5.4e-128	0.9959677419354839

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
133054	GT8_HUGT1_C_like	0.0	1329	1576	1	248	The C-terminal domain of HUGT1-like is highly homologous to the GT 8 family. C-terminal domain of glycoprotein glucosyltransferase (UGT). UGT is a large glycoprotein whose C-terminus contains the catalytic activity. This catalytic C-terminal domain is highly homologous to Glycosyltransferase Family 8 (GT 8) and contains the DXD motif that coordinates donor sugar binding, characteristic for Family 8 glycosyltransferases. GT 8 proteins are retaining enzymes based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. The non-catalytic N-terminal portion of the human UTG1 (HUGT1) has been shown to monitor the protein folding status and activate its glucosyltransferase activity.
408203	Glyco_transf_24	0.0	1329	1595	1	268	Glucosyltransferase 24. This is the catalytic domain found in UDP-glucose:glycoprotein glucosyltransferase (UGGT). This domain belongs to glucosyltransferase 24 family (GT24) A-type domain. The GT domain displays the expected glycosyltransferase type A (GT-A) fold.
408201	Thioredoxin_14	1.39e-65	459	714	1	248	Thioredoxin-like domain. This is the third out of four TRXL(thioredoxin-like) domains found in UDP-glucose:glycoprotein glucosyltransferase (UGGT).
132996	Glyco_transf_8	1.11e-55	1329	1576	1	246	Members of glycosyltransferase family 8 (GT-8) are involved in lipopolysaccharide biosynthesis and glycogen synthesis. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. GT-8 comprises enzymes with a number of known activities: lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, glycogenin glucosyltransferase, and N-acetylglucosaminyltransferase. GT-8 enzymes contains a conserved DXD motif which is essential in the coordination of a catalytic divalent cation, most commonly Mn2+.
399437	UDP-g_GGTase	3.76e-52	1179	1288	1	109	UDP-glucose:Glycoprotein Glucosyltransferase. UDP-g_GGTase is an important, central component of the QC system in the ER for checking that glycoproteins are folded correctly. This QC prevents incorrectly folded glycoproteins from leaving the ER.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AAW41515.2|GT24	0.0	37	1637	20	1573
KGB74213.2|GT24	0.0	37	1621	20	1557
AUB22656.1|GT24	0.0	37	1621	20	1557
AFR93153.2|GT24	0.0	37	1621	20	1557
UOH81582.1|GT24	0.0	37	1621	20	1560

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5MU1_A	3.26e-268	32	1603	3	1455	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5MZO_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TRF_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
5NV4_A	1.78e-267	32	1603	3	1455	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TRT_A	3.50e-267	32	1603	3	1455	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TS8_A	1.28e-234	37	1538	1	1382	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS8_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TS2_A	6.77e-215	32	1603	3	1221	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_C Chain C, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_D Chain D, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q09140|UGGG_SCHPO	1.20e-198	39	1603	21	1435	UDP-glucose:glycoprotein glucosyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=gpt1 PE=1 SV=2
sp|Q09332|UGGG_DROME	8.72e-190	15	1603	5	1515	UDP-glucose:glycoprotein glucosyltransferase OS=Drosophila melanogaster OX=7227 GN=Uggt PE=1 SV=2
sp|Q6P5E4|UGGG1_MOUSE	6.29e-183	40	1592	47	1520	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Mus musculus OX=10090 GN=Uggt1 PE=1 SV=4
sp|Q9NYU2|UGGG1_HUMAN	4.86e-182	36	1598	43	1526	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Homo sapiens OX=9606 GN=UGGT1 PE=1 SV=3
sp|Q9JLA3|UGGG1_RAT	1.65e-181	40	1592	47	1520	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Rattus norvegicus OX=10116 GN=Uggt1 PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.004514	0.995429	CS pos: 28-29. Pr: 0.9582

TMHMM  Annotations     

Start	End
7	29