CAZyme Information: I317_06629-t43_1-p1

Basic Information

• Species: Kwoniella heveanensis
• Lineage: Basidiomycota; Tremellomycetes; ; Cryptococcaceae; Kwoniella; Kwoniella heveanensis
• CAZyme ID: I317_06629-t43_1-p1
• CAZy Family: GT4
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
866		92637.31	5.3793

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_KheveanensisCBS569	8002	1296119	131	7871

• Gene Location: location=KV700254:27834-31152(-)

Full Sequence      

MAPNYPWARSQPSGSSSSSASGSAAGASTPTQRSIDSRPVVVSVSGRSANPANSPSVNGA
GAGAPGGNLNIDSPSTLRNRVASPRYSNTITQANVDVFQSESSSYPSPTESKTKEKEKGK
VKRKSFLGTGLGIGSGSGGGNGNGSEKAKEKKGWYELSSTSPSAKAAAQGLPVPRYDSRG
YPTREQSGHLAVDSSHAQQSPEATRSSYRYVGSSSYGHGYDEGNGNGYAHTYRHENGDGS
GGGTGSIYGQGIKPSRSIDSLSSEVSSTGAGVNEETSRFISSSSSSDPPTSYYLPSYHTH
SGSSSGGSTLPTPHSLYPPTLDYSLSAPSYSPYDNGGVSSNPNPLPSLYGDSRVSLNSMQ
SGESMTHFRQFDSLKSSGGGGQGAPGYGYSNFSISSKGGKRHLADMFSLPADPATWSHLG
PEEDDDFHDPDFKPKRKSRFGSSIFTLRGAANLGCLFAMLLVLVVLFAGYPILDNYLAKG
LKSHGAYNLGGINATGQIPVIDAFSIIDKDTPQSAYTWTSLETGEEWELVFSDEFNRDGR
TFFDGDDPYWQAVDLHYWQTNNLEWYDPRQVTTKDGNLVITLDKIKNHGLDYMGGMIQTW
NRFCFTGGYVEASVSLPGTSNVYGLWPAIWTLGNLGRAGYGGTLDGLWPYSYDVCDLGTL
PNQTLKGEPALTLTDGDPNYGGVLSYLPGQRLSRCTCENDATHPGPKLSNGTFRGRSAPE
IDMFEAAVDQDTLIGHVSQSAQWAPYNPHYTFINTSTKDYEIYDSSVTTINTYMGSVYQQ
ATSGLSTTDQKCYTGDTGCLSVYGFEYSPGVDGYITWVNDNKKAWTIRGPAMGPNEDAMV
GQRHVPDEPMYLIINLGISENFGAIE

Enzyme Prediction     

No EC number prediction in I317_06629-t43_1-p1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH16	533	865	2.4e-127	0.9386503067484663

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
397841	SKN1	0.0	421	866	8	430	Beta-glucan synthesis-associated protein (SKN1). This family consists of the beta-glucan synthesis-associated proteins KRE6 and SKN1. Beta1,6-Glucan is a key component of the yeast cell wall, interconnecting cell wall proteins, beta1,3-glucan, and chitin. It has been postulated that the synthesis of beta1,6-glucan begins in the endoplasmic reticulum with the formation of protein-bound primer structures and that these primer structures are extended in the Golgi complex by two putative glucosyltransferases that are functionally redundant, Kre6 and Skn1. This is followed by maturation steps at the cell surface and by coupling to other cell wall macromolecules.
185689	GH16_fungal_KRE6_glucanase	2.40e-135	529	866	1	276	Saccharomyces cerevisiae KRE6 and related glucanses, member of glycosyl hydrolase family 16. KRE6 is a Saccharomyces cerevisiae glucanase that participates in the synthesis of beta-1,6-glucan, a major structural component of the cell wall. It is a golgi membrane protein required for normal beta-1,6-glucan levels in the cell wall. KRE6 is closely realted to laminarinase, a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
225182	BglS	1.50e-37	505	861	28	318	Beta-glucanase, GH16 family [Carbohydrate transport and metabolism].
185693	GH16_laminarinase_like	5.63e-21	531	863	1	214	Laminarinase, member of the glycosyl hydrolase family 16. Laminarinase, also known as glucan endo-1,3-beta-D-glucosidase, is a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
185683	Glyco_hydrolase_16	5.22e-08	533	645	1	102	glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AUB26017.1|GH16_2	1.61e-215	400	865	343	805
AFR96134.2|GH16_2	1.61e-215	400	865	343	805
KGB75891.2|GH16_2	7.41e-214	394	865	338	806
ADV23033.1|GH16_2	8.07e-213	400	865	344	806
QRV97496.1|GH16_2	8.64e-206	396	866	110	582

PDB Hits     

I317_06629-t43_1-p1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|O13941|YEPB_SCHPO	6.62e-94	415	862	125	551	Uncharacterized beta-glucan synthesis-associated protein C23H3.11c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC23H3.11c PE=3 SV=1
sp|P87024|SKN1_CANAX	1.74e-90	389	866	222	668	Beta-glucan synthesis-associated protein SKN1 OS=Candida albicans OX=5476 GN=SKN1 PE=2 SV=1
sp|P32486|KRE6_YEAST	3.20e-89	422	866	217	641	Beta-glucan synthesis-associated protein KRE6 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=KRE6 PE=1 SV=2
sp|P33336|SKN1_YEAST	3.36e-84	419	866	253	693	Beta-glucan synthesis-associated protein SKN1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SKN1 PE=1 SV=1
sp|P87023|KRE6_CANAX	1.71e-82	390	866	208	671	Beta-glucan synthesis-associated protein KRE6 OS=Candida albicans OX=5476 GN=KRE6 PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000087	0.000002

TMHMM  Annotations     

Start	End
449	471